A5025354363- Animal Nutrition and Physiology
- Probiotics and Fermented Foods
- Gut microbiota and health
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Microtubule and mitosis dynamics
- Algal biology and biofuel production
- Fermentation and Sensory Analysis
- Trace Elements in Health
- Essential Oils and Antimicrobial Activity
- Meat and Animal Product Quality
- Cardiovascular Conditions and Treatments
- Coronary Artery Anomalies
- Yeasts and Rust Fungi Studies
- Genomics and Chromatin Dynamics
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Pharmaceutical and Antibiotic Environmental Impacts
- Cardiovascular Disease and Adiposity
- Angiogenesis and VEGF in Cancer
- Impact of Light on Environment and Health
- Animal Behavior and Welfare Studies
- Bacteriophages and microbial interactions
University of Virginia
2011-2022
Freie Universität Berlin
2009-2017
Federal Institute for Risk Assessment
2012-2015
Research Institute for Farm Animal Biology (FBN)
2006-2013
University of Rostock
2011-2013
Zimmer Biomet (Germany)
2012
Leibniz Association
2011
Landesforschungsanstalt für Landwirtschaft und Fischerei Mecklenburg-Vorpommern
2009
Warsaw University of Life Sciences
2004
Background Animal faeces comprise a community of many different microorganisms including bacteria and viruses. Only scarce information is available about the diversity viruses present in pigs. Here we describe protocol, which was optimized for purification total fraction viral particles from pig faeces. The genomes purified DNA RNA were simultaneously amplified by PCR subjected to deep sequencing followed bioinformatic analyses. efficiency method monitored using process control consisting...
New treatment strategies for inflammatory bowel disease are needed and parasitic nematode infections or application of helminth components improve clinical experimental gut inflammation. We genetically modified the probiotic bacterium Escherichia coli Nissle 1917 to secrete powerful immunomodulator cystatin in gut. This was tested a murine colitis model on post-weaning intestinal inflammation pigs, an outbred with gastrointestinal system similar humans. Application transgenic significantly...
The spindle- and kinetochore-associated (Ska) complex is essential for normal anaphase onset in mitosis. C-terminal domain (CTD) of Ska1 binds microtubules was proposed to facilitate kinetochore movement on depolymerizing spindle microtubules. Here, we show that Ska recruits protein phosphatase 1 (PP1) kinetochores. This recruitment requires the CTD, which PP1 vitro human HeLa cells. lacking its CTD fused a PP1-binding peptide or directly rescues mitotic defects caused by depletion. fusion...
Abstract Glioblastoma is a deadly cancer, with no effective therapies. Better understanding and identification of selective targets are urgently needed. We found that advillin (AVIL) overexpressed in all the glioblastomas we tested including glioblastoma stem/initiating cells, but hardly detectable non-neoplastic astrocytes, neural stem cells or normal brain. Glioma patients increased AVIL expression have worse prognosis. Silencing nearly eradicated culture, dramatically inhibited vivo...
Our study aimed to provide a comprehensive characterization of changes in porcine intestinal Lactobacillus populations around the time weaning based on 16S rRNA gene amplification and denaturing gradient gel electrophoresis (DGGE). DNA was extracted from ileal contents piglets at (28 days age) after 1, 2, 5 11 days. PCR amplicons (V2-V3 fragments genes) were separated using DGGE. Predominant bands excised sequenced reamplification. A band corresponding salivarius present 1 2 post-weaning...
It is generally accepted that the intestinal microbiota plays an important role in sustaining health and productivity of animals. Chlorella vulgaris, a naturally occurring green microalga, believed to influence performance health, including bird reproduction egg quality. The nutritive value open or indoor cultured C. vulgaris depends upon technological process used treat algal mass. In present paper, it presented discussed how 2 differentially processed powders (spray-dried: SD-CV;...
Highlights•The Ndc80 complex orients the Ska along microtubule protofilaments•The unstructured tail recruits to kinetochore•Structural view of a putative metazoan kinetochore-microtubule attachment•Clusters Ndc80, which are seen on microtubules in vitro, may function vivoSummaryYeast use ring-shaped Dam1 slide down depolymerizing move chromosomes, but current models suggest that other eukaryotes do not have sliding ring. We visualized and complexes by electron microscopic tomography identify...
Zinc (Zn) supplementation has been shown to reduce the incidence of diarrhea and protect animals from intestinal diseases, but mechanisms this protective effect against virus infection in vivo have not yet elucidated. Transmissible gastroenteritis (TGEV) causes piglets with an age-dependent decrease severity. We used 60 weaned that were divided into three groups evaluate different Zn levels added a conventional diet (50 mg Zn/kg diet, Znlow, control group). The other received supplemented...
The effects of inulin and alginate on intestinal microbial ecophysiology were investigated in piglets fed a diet (C) with 0.1% (C+A) or 1.5% (C+I) from weaning at day 28. experiment was performed an experimental farm (EF) commercial (CF). Digesta collected the ileum, caecum colon four each group days 29, 30, 33 39. metabolite concentrations changed age. Colonic caecal metabolites affected by prebiotic treatment. Changes microbiota composition assessed cultivation denaturing gradient gel...
This study aimed to characterize the intestinal yeasts in weaning piglets and establish their possible relationships with main bacterial groups. German Landrace were weaned (WP, n=32) at 28 days of age or kept dams until day 39 without creep feed (UP, n=32). The experiment was performed an experimental a commercial farm (CF). Faeces collected from piglets, sows pen floors on 28, 33 for isolation DNA cultivation enumeration yeasts, enterobacteria, enterococci lactobacilli. Fragments D1 domain...
Salmonellae are among the most widespread sources of foodborne infections and Salmonella Typhimurium, in particular, is correlated with human disease caused by consumption contaminated pork. Intraepithelial lymphocytes (IEL) have early contact intestinal antigens play an important role detection pathogenic bacteria. The objective this study was to determine whether a presumed probiotic Enterococcus faecium strain could improve histomorphological immune system-related parameters gut function...
Salmonella enterica serovar Typhimurium DT 104 is the major pathogen for salmonellosis outbreaks in Europe. We tested if probiotic bacterium Enterococcus faecium NCIMB 10415 can prevent or alleviate salmonellosis. Therefore, piglets of German Landrace breed that were treated with E. (n = 16) as a feed additive and untreated controls challenged S. 10 days after weaning. The presence salmonellae feces selected organs, well immune response, investigated. Piglets gained less weight than control...
The study was conducted to determine yeasts present in the gastrointestinal tract (GIT) of piglets kept under experimental farm (EF) and commercial (CF) conditions. Ninety five German Landrace full- half-sibling were sacrificed at 39 days age. Sixty eight weaned 28th day life, when they offered one diet ad libitum. Twenty seven remained unweaned by their dams. None received any creep feed before weaning. Digesta samples collected from 1/3 distal small intestine (SI), caecum proximal colon....
This study investigated the impact of zinc oxide (ZnO) on Campylobacter coli by in vivo and vitro assays.By growth inhibition assays, a high susceptibility Camp. against ZnO could be observed. At concentrations ≥ 2.6 mmol l(-1) ZnO, decline cell numbers occurred. Quantitative real-time PCR assays demonstrated an up-regulation main oxidative stress gene (katA) response to treatment. The expression level katA was increased fivefold after An experiment carried out pigs elucidate as feed...