A5069152585- MRI in cancer diagnosis
- Pediatric Hepatobiliary Diseases and Treatments
- Advanced MRI Techniques and Applications
- Urologic and reproductive health conditions
- Gallbladder and Bile Duct Disorders
- Urological Disorders and Treatments
- Bladder and Urothelial Cancer Treatments
- Urinary and Genital Oncology Studies
- Renal Diseases and Glomerulopathies
- Fetal and Pediatric Neurological Disorders
- Diagnosis and treatment of tuberculosis
- Enzyme Production and Characterization
- Diverticular Disease and Complications
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Breast Lesions and Carcinomas
- Abdominal Trauma and Injuries
- Head and Neck Cancer Studies
- Infectious Disease Case Reports and Treatments
- Colorectal and Anal Carcinomas
- Infectious Diseases and Tuberculosis
- IgG4-Related and Inflammatory Diseases
- Soft tissue tumor case studies
- Hemoglobinopathies and Related Disorders
Japanese Red Cross Medical Center
1998-2024
Nihon University
1998
Hoshi University
1983-1997
Ebara (Japan)
1996
Women Medical College
1992
Tokyo Women's Medical University
1990
Three alkaline ribonucleases [EC 3.1.4.22] were purified 4,500- to 7,850-fold from bovine parotid gland by repeated CM-Sephadex C-25 chromatography and Sephadex G-50 gel filtration, with a total recovery of about 33%. They designated as RNase BP1, BP2, BP3, based on their order elution column. The molecular weights these enzymes estimated electrophoresis be 18,500, 18,000, 14,000, respectively. These are very similar A in that they inhibited heparin, show preferential hydrolysis C5'-O-P...
The presumed etiology and prevalence of pancreatic abnormalities in patients with ulcerative colitis (UC) have been controversial. We conducted a controlled (cross-sectional) study UC compared non-UC controls to determine the duct if these are specific using magnetic resonance cholangiopancreatography.Magnetic cholangiopancreatography was performed on 79 unselected 45 controls, without history pancreatitis attack, between February 2000 May 2003.Among UC, 16.4% (95% confidence interval,...
Sixty-one CT scans in 20 patients who had undergone head and neck reconstructive surgery using a pectoralis major myocutaneous (PM-MC) flap were retrospectively evaluated to assess the usefulness of follow-up these patients. The normal findings with PM-MC flaps are described. Of 13 cases tumor recurrence, examination correctly detected recurrent masses all cases, including 3 which there was no clinical sign recurrence. Postoperative mimicking recurrence included deformed breast tissue,...
In order to study the reaction mechanism of RNase Rh from Rhizopus niveus, rates cleavage four 2',3'-cyclic nucleotides by mutant enzymes Rh, H46F, H109F, E105Q, and K108L were measured. H46F is virtually inactive towards cyclic nucleotides, but H109F hydrolyzed these substrates at 0.7-4.5% native Rh. The other mutants 15-20% enzyme. Relative enzymatic activities in hydrolysis (2nd step) much higher than transphosphorylation (the 1st step). presence a 13-fold excess uridine, catalyzed AMP...
A base non-specific and acid RNase was isolated from cellular slime mold (Dictyostelium discoideum) cells in a homogeneous state (about 2.4 kDa) by SDS-polyacrylamide gel electrophoresis. The (RNase Ddl) has pH optimum of 5.0. amino sequence Ddl determined combination protein chemistry, search Data base, Dicty cDB further analysis cDNA the same bank. consists 198 residues, about 13.3, 0.9, 1.2, 3.3, 1.0 residues mannose, xylose, glucose, GlcNAc, GalNAc, respectively. two characteristic...
In order to establish the role of Tyr57 and Trp49 in enzymatic reaction RNase Rh, several mutant enzymes at were prepared by protein engineering their properties investigated. Among four (W49F, W49Y, W49A, W49I), W49F showed 16% activity native enzyme, but others (W49Y, W49I) greatly decreased activity. The data that is very important for enzyme 8 57th position, Y57F Y57W similar toward RNA wild-type (Y57G, Y57A, Y57V, Y57M, Y57K) are more active less XpGs. reason apparent increase discussed...
We previously investigated the role of Lys108 residue ribonuclease (RNase) Rh from Rhizopus niveus, and suggested that probably acts to stabilize pentacovalent intermediate, an Arg could replace Lys108. In RNase Le2 Lentinus edodes, a homologous enzyme Rh, is replaced by Thr. this paper, enzymatic properties K108T mutant its analogous enzyme, K108S, were determine effect Thr analog, Ser at 108th position on activity. The these enzymes compared with those other (K108M, K108A, K108L). results...
Purpose: The kidney iron deposition can cause damage and renal insufficiency in paroxysmal nocturnal hemoglobinuria (PNH) patients. Assessment of the is essential for early diagnosis PNH purpose this study was to evaluate R2* (T2* reciprocals) values patients using iterative decomposition water fat with echo asymmetry least-squares estimation (IDEAL-IQ).
Journal Article Enzymatic Properties of Mutant Forms RNase Rh from Rhizopus niveus as to Asp51 Get access Kazuko Ohgi, Ohgi Department Microbiology, Hoshi College Pharmacy, Ebara 2-4-41, Shinagawa-ku, Tokyo 142 Search for other works by this author on: Oxford Academic PubMed Google Scholar Mitsuaki Takeuchi, Takeuchi Masanori Iwama, Iwama Masachika Irie 2 2To whom correspondence should be addressed. The Biochemistry, Volume 119, Issue 3, March 1996, Pages 548–552,...
Plasmacytoid urothelial carcinomas of the bladder are rare, aggressive variants with a poor prognosis. Few reports have described correlation histopathological features multiparametric magnetic resonance imaging findings in local staging plasmacytoid carcinoma. An 82-year-old woman hematuria was referred to our hospital. Magnetic showed diffuse wall thickening, different signal intensities 2 layers-inner and outer. This case suggests that presence thickening varying layers could aid A...