A5110726787- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Viral Infections and Immunology Research
- interferon and immune responses
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Microbial infections and disease research
- Genetic diversity and population structure
- Viral Infections and Vectors
- Digital and Cyber Forensics
- Mosquito-borne diseases and control
- Viral Infectious Diseases and Gene Expression in Insects
- Immunodeficiency and Autoimmune Disorders
- Hepatitis B Virus Studies
- Acute Kidney Injury Research
- Virology and Viral Diseases
- Vector-borne infectious diseases
- Bacteriophages and microbial interactions
- Plant Reproductive Biology
Sichuan Agricultural University
2014-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Weifang People's Hospital
2019-2023
National Animal Husbandry Service
2022
First Affiliated Hospital of Xinjiang Medical University
2020
Xinjiang Medical University
2020
Agriculture and Agri-Food Canada
2020
Jiangxi Normal University
2016
Henan Agricultural University
2015
Weinan Normal University
2012
Common oat (Avena sativa) is an important cereal crop serving as a valuable source of forage and human food. Although reference genomes many crops have been generated, such work in has lagged behind, primarily owing to its large, repeat-rich polyploid genome. Here, using Oxford Nanopore ultralong sequencing Hi-C technologies, we generated reference-quality genome assembly hulless common oat, comprising 21 pseudomolecules with total length 10.76 Gb contig N50 75.27 Mb. We also produced...
Abstract Malnutrition and acute kidney injury (AKI) are common complications in hospitalised patients, both increase mortality; however, the relationship between them is unknown. This a retrospective propensity score matching study enrolling 46 549 inpatients, aimed to investigate association Nutritional Risk Screening 2002 (NRS-2002) AKI assess ability of NRS-2002 predicting prognosis. In total, 37 190 (80 %) 9359 (20 patients had scores <3 ≥3, respectively. Patients with ≥3 longer...
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that poses a serious threat to the global pig industry. Sichuan Province one of largest breeding provinces in China. There lack reports on continuous surveillance systematic analysis prevalent strains PRRSV recent years. To fill this gap, total 539 samples were collected from 13 regions during 2012–2020. The detection result showed positive rate was 52.32% (282/539). ORF5s Nsp2 obtained further analyzed,...
Pseudorabies virus can cause inflammation in the central nervous system and neurological symptoms. To further investigate protective mechanism of PRV XJ delgE/gI/TK system, an intracranial PRV-infection mice model was developed. The results demonstrated that immunization with successfully prevented death caused by PRV-intracranial infection. Subsequently, brains were collected for transcriptome metabolome analysis. GO KEGG enrichment analysis indicated differentially expressed genes...
Porcine deltacoronavirus (PDCoV) is a new pathogenic porcine intestinal coronavirus, which has appeared in many countries since 2012. PDCoV disease caused acute diarrhea, vomiting, dehydration and death piglets, resulted significant economic loss to the pig industry. However, there no commercially available vaccine for PDCoV. In this study, we constructed recombinant pseudorabies virus (rPRVXJ-delgE/gI/TK-S) expressing spike (S) protein evaluated its safety immunogenicity mice.The strain...
ABSTRACT Aspergillus fumigatus is the most common species that causes invasive aspergillosis. In order to identify A. , partial ribosomal DNA (rDNA) from two six strains of five different was sequenced. By comparing sequence data GenBank, we designed specific primer pairs targeting rDNA internal transcribed spacer (ITS) regions . A nested PCR method for identification other -related established by using primers. To evaluate specificities and sensitivities those primers, 24 isolates variants,...
Porcine reproductive and respiratory syndrome (PRRS) pseudorabies (PR) are highly infectious swine diseases cause significant financial loss in China. The system the main target systems. Previous studies showed that existing PR virus (PRV) PRRS (PRRSV) commercial vaccines could not provide complete protection against PRV variant strains NADC30-like PRRSV In this study, strain XJ CHSCDJY-2019 used as parent for constructing a recombinant (rPRV)-NC56 with gE/gI/TK gene deletion co-expressing...
Based on a variant strain, we constructed gE/gI/TK-deleted pseudorabies virus (PRV). A total of 18 female mice were randomized to vaccination group receive PRV XJ delgE/gI/TK, vehicle Dulbecco's modified Eagle's medium, and mock confirm the protection delgE/gI/TK central nervous system in mice. Subsequently, groups infected with XJ. The showed more severe neurological symptoms higher viral loads than those group. exudation Evans blue expression tight junction protein no difference all...
Acute kidney disease (AKD) defines patients with acute injury (AKI) or subacute loss of function lasting for >7 days. Little is known about the prognosis AKD in hospitalized patients. The aim this study was to investigate risk factors and compare different types acute/subacute renal impairment among Chinese inpatients. Complete data were available 71 041 a range 5-63 months. AKI diagnosed based on Disease Quality Initiative criteria 2017. Of inpatients, 16 098 (22.7%) developed AKD; 5895...
Pseudorabies is a disease that seriously endangers the pig industry in China. Recently, we successfully isolated pseudorabies virus from brain tissue of piglets at farm Sichuan, China, and named it FJ62 strain. In order to understand molecular biological characteristics strain, primers were designed for glycoproteins gB, gC, gD gE, which amplified by polymerase chain reaction (PCR) sequenced. After comparing sequence with GenBank 22 reference strains establishing genetic evolutionary tree,...
Seneca Valley virus (SVV), a non-enveloped positive single-stranded can cause vesicular disease in swine. However, the mechanisms by which SVV activates an innate immune response remain unknown. Mitofusin-2 (MFN2), mitochondria-shaping protein regulating mitochondrial fusion and fission, plays crucial role responses. But, roles of Mfn2 infection have not been elucidated. Here, we show that inhibited expression NLRP3 inflammasome, activating RIG-I/IRF7 signaling pathway to increase IFN-λ3...
Two uncorrelated nucleotide sequences, chloroplast intergenic spacer psbA–trnH and acetyl CoA carboxylase gene (Acc1), were used to perform phylogenetic analyses in 75 accessions of the genus Avena, representing 13 diploids, seven tetraploid, four hexaploids by maximum parsimony Bayesian inference. Phylogenic based on confirmed that A genome diploid might be maternal donor species Avena. haplotypes Acc1 region obtained from AB tetraploids, indicating an allopolyploid origin for tetraploid...
Cold stress is one of the major restraints for rice production. tolerance controlled by complex genetic factor. In this study, a backcross inbred lines (BILs) population derived from an inter-specific cross (Oryza sativa L.×O. rufipogon Griff.) was used linkage map construction and quantitative trait locus (QTL) mapping. A consisting 153 markers constructed, spanning 1 596.8 cM with average distance 11.32 between adjacent markers. Phenotypic evaluation parents BILs under (6±1)°C cold...
Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) is a serious viral disease of swine. At present, there are vaccines for the control PRRSV infection, but effect not satisfactory. The recombination attenuated causes significant difficulties with prevention PRRSV. Type III interferons (IFNs), also called IFN-λs, were newly identified showed potent antiviral activity within mucosal surface immune organs. Results Therefore, primary porcine alveolar macrophages...
Background: In this study, we aim to investigate the efficiency of artesunate (AS) on Echinococcus granulosus protoscoleces and metacestodes. Methods: For in vitro assay, eosin dye exclusion test transmission electron microscope (TEM) were utilized evaluate effects AS against (PSCs) from . addition, mortality, ultrastructure change, reactive oxygen species (ROS) content DNA damage measured order explore anti-echinococcosis mechanism AS. vivo CE-infected mice divided into model group,...
Abstract Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating viral diseases in global pig industry, including China. Recently, we successfully isolated a porcine virus (PRRSV) from lung tissue peripheral blood piglets at farm Dujiangyan Sichuan, China, named it DJY‐19 strain. The full‐length genome sequence shared 86.8%–94.1% nucleotide similarity with NADC30‐like NADC30 PRRSV strains. We compared open reading frame (ORF) 5 gene 34 strains...
Abstract Background Hepatitis E virus (HEV) is an important zoonotic pathogen, Genotypes 3 and 4 are the main genotype. Due to lack of mature effective culture cell lines, researches on genotype IV swine HEV (SHEV‐4) infection pathogenic mechanism have been carried out in pigs, gerbils non‐human primate models. Objectives The aim this study was establish a rat model by intra‐peritoneal with SHEV‐4, which provided new research idea scientific basis for further revealing preventing infection....
A novel porcine reproductive and respiratory syndrome virus (PRRSV) strain with 393 nucleotide deletions in the nonstructural protein 2 (Nsp2) region was examined this study. Results will help improve our understanding of epidemiology genetic diversity North American-type PRRSV China.
Crown rust, caused by Puccinia coronata f. sp. avenae Eriks. (Pca), is among the most important oat diseases resulting in significant yield losses many growing regions. A gene-for-gene interaction well established this pathosystem and has been exploited breeders to control crown rust. Pc39 a seedling rust resistance gene that widely deployed North American breeding. DNA markers are desired accurately predict specific Pc genes present breeding germplasm. The objectives of study were as...
The complexity of the pathogenic mechanism underlying host immune response to Actinobacillus pleuropneumonia (App) makes use preventive measures difficult, and a more global view host-pathogen interactions new insights into this process are urgently needed reveal mechanisms App infection. Here, we infected specific pathogen-free Mus musculus with serotype 7 by intranasal inoculation construct an acute hemorrhagic pneumonia infection model isolated lungs for analysis dual RNA-seq.Four cDNA...
Senecavirus A (SVA) is a new type of virus related to swine vesicular disease, which results in enormous economic losses worldwide. At present, the host transcriptional responses SVA infection, host-SVA interactions, and mechanism innate immune modulation are not well understood. This study explores gene expression profiles PK-15 cells at 0, 6, 12, 18, 24, 36 h post-infection by RNA sequencing. Our analysis identified 61, 510, 1,584, 2,460, 2,359 differentially expressed genes (DEGs)...