A5090688930- Advanced biosensing and bioanalysis techniques
- Genomic variations and chromosomal abnormalities
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Lung Cancer Treatments and Mutations
- DNA and Nucleic Acid Chemistry
- HER2/EGFR in Cancer Research
- Cervical Cancer and HPV Research
- Prenatal Screening and Diagnostics
- Cancer Research and Treatments
- Esophageal Cancer Research and Treatment
- Lung Cancer Research Studies
- Photodynamic Therapy Research Studies
- Gastric Cancer Management and Outcomes
- Cell Image Analysis Techniques
- Cancer-related Molecular Pathways
- Gastrointestinal Tumor Research and Treatment
- Advanced Biosensing Techniques and Applications
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Chromosomal and Genetic Variations
- Colorectal Cancer Treatments and Studies
- Photosynthetic Processes and Mechanisms
- Cutaneous Melanoma Detection and Management
- PI3K/AKT/mTOR signaling in cancer
Ventana Research Corporation (United States)
2011-2025
Roche (United States)
2016-2025
Abbott (United States)
2006-2017
Montana Molecular (United States)
2008-2014
Abbott Fund
2003-2011
Abbott (Sweden)
2008
Kanazawa University
2007
AstraZeneca (United Kingdom)
2007
Abbott (United Kingdom)
2002-2007
University of Chicago
2006
Abstract Purpose: The purpose of this study was to test the hypothesis that circulating tumor cells (CTCs) are present in patients many years after mastectomy without evidence disease and these CTCs shed from persisting with breast cancer dormancy. Experimental Design: We searched for 36 dormancy candidate 26 age-matched controls using stringent criteria cytomorphology, immunophenotype, aneusomy. Results: Thirteen candidates, 7 22 clinical disease, had CTCs, usually on more than one...
Amplification and overexpression of the HER-2 oncogene in breast cancer is felt to be stable over course disease concordant between primary tumor metastases. Therefore, patients with -negative tumors rarely will receive anti-Her-2 antibody (trastuzumab, Herceptin) therapy. A very sensitive blood test was used capture circulating cells (CTCs) evaluate their gene status by fluorescence situ hybridization. The corresponding CTCs 31 showed 97% agreement, no false positives. In 10 -positive...
Although the clinical and pathologic diagnosis of some melanomas is clear-cut, there are many histopathologic simulators melanoma that pose problems. Over-diagnosis can lead to inappropriate therapy psychologic burdens, whereas under-diagnosis inadequate treatment a deadly cancer. We used existing data on DNA copy number alterations in assemble panels fluorescence situ hybridization (FISH) probes suitable for analysis paraffin-embedded tissue. Using FISH from training set 301 tumors, we...
The present preimplantation diagnosis test is able to screen for the most common aneuploidies from single blastomeres in about five hours with a 15 per cent misdiagnosis. This means that risk of spontaneous abortion and trisomic offspring women advanced maternal age could be reduced same level as younger whom prenatal usually not necessary. Better probes more fluorochromes improve success rate test. Copyright © 1998 John Wiley & Sons, Ltd.
Kinetic and thermodynamic constants associated with DNA hybridization were determined in solution using fluorescence measurements complementary fluorophore-labeled oligomers. One oligomer was labeled a 5'-terminal fluorescein, the other 3'-terminal rhodamine. The juxtaposition of two labels double-stranded complexes results strong quenching fluorescein emission, thereby providing means for distinguishing single-stranded from DNA. Since based on fluorescence, denaturation association could be...
Overexpression of urokinase plasminogen activator system or HER-2 (erbB-2) in breast cancer is associated with a poor prognosis. overexpression caused by gene amplification. The anti-HER-2 antibody trastuzumab significantly improves clinical outcome for HER2-positive cancer. Drugs that target the uPA are early trials. aims this study were to determine whether receptor (uPAR) amplification occurs and analysis individual tumor cells (TCs) blood tissue can add information conventional...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and characterization of DNA oligomers duplexes containing covalently attached molecular labels: comparison biotin, fluorescein, pyrene labels by thermodynamic optical spectroscopic measurementsJoshua Telser, Kenneth A. Cruickshank, Larry E. Morrison, Thomas L. NetzelCite this: J. Am. Chem. Soc. 1989, 111, 18, 6966–6976Publication Date (Print):August 1, 1989Publication History Published online1 May 2002Published inissue 1 August...
Fluorescence in-situ hybridization (FISH) for application in preimplantation genetic diagnosis (PGD) of aneuploidy has been used successfully, but stringent scoring criteria to score FISH signals have not developed. In the present study a protocol simultaneously enumerate chromosomes X, Y, 13, 16, 18, and 21 was evaluate two different criteria. The consider signal size, shape, vicinity other nuclear diameter. For this purpose, 74 embryos (412 blastomeres) donated research had most or all...
Relating specific genetic alterations to prognosis may help improve prognostication in melanoma, identify key oncogenic drivers cancer, and assist developing targeted therapies. Characteristic melanoma include chromosomal copy number aberrations. We evaluated 97 melanomas (55 metastasizing 42 nonmetastasizing) after a minimum 5-year follow-up case-control study using fluorescence situ hybridization, targeting commonly altered loci melanoma. Eight probes arranged two panels were used, 11...
Brightfield microscopy is the preferred method of pathologists for diagnosing solid tumors, utilizing common staining techniques such as hematoxylin and eosin immunohistochemistry (IHC). However, our understanding complex tumor microenvironment grows, there increasing demand multiplexed biomarker detection. Currently, IHC assays are almost exclusively based on immunofluorescence because brightfield limited by broad spectral absorption chromogens a reliance conventional 3-channel color...
Background/Objectives: Performing hematoxylin and eosin (H&E) staining immunohistochemistry (IHC) on the same specimen slide provides advantages that include conservation ability to combine H&E context with biomarker expression at individual cell level. We previously used invisible deposited chromogens dual-camera imaging, including monochrome color cameras, implement simultaneous IHC. Using this approach, conventional could be simultaneously viewed in a computer monitor alongside...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDNA duplexes covalently labeled at two sites: synthesis and characterization by steady-state time-resolved optical spectroscopiesJoshua Telser, Kenneth A. Cruickshank, Larry E. Morrison, Thomas L. Netzel, Chi Kin ChanCite this: J. Am. Chem. Soc. 1989, 111, 18, 7226–7232Publication Date (Print):August 1, 1989Publication History Published online1 May 2002Published inissue 1 August...
Conventional histological stains, such as hematoxylin plus eosin (H&E), and immunohistochemistry (IHC) are mainstays of histology that provide complementary diagnostic information. H&E IHC currently require separate slides, because the stains would otherwise obscure one another. This consumes small specimen, limiting total amount testing. Additionally, performing on different slides does not permit comparison staining at single cell level, since same cells present each slide, alignment...
Preimplantation genetic diagnosis (PGD) of numerical chromosome abnormalities significantly reduces spontaneous abortions and may increase pregnancy rates in women advanced maternal age undergoing vitro fertilization. However, the technique has an error rate around 10% trisomy 21 conceptions have occurred after PGD. To further reduce risk transferring embryos to patient, we designed a protocol that analyzes twice by targeting two different loci. This was applied 388 from 60 cycles PGD...