The P2Y₁₄ receptor was initially identified as a G protein-coupled activated by UDP-glucose and other nucleotide sugars. We have developed several cell lines that stably express the human receptor, allowing facile examination of its coupling to native G_i family proteins their associated downstream signaling pathways (<i>J Pharmacol Exp Ther</i><b>330:</b>162–168, 2009). In current study, we examined receptor-dependent inhibition cyclic AMP accumulation in embryonic...

Mycobacterium tuberculosis PhoP is essential for virulence and intracellular growth of the tubercle bacilli. Genetic evidence suggests that regulates complex lipid biosynthesis, absence some these molecules in a phoP mutant partly accounts its attenuated macrophages and/or mice. To investigate mechanism regulation, here we demonstrate essentiality phosphorylation regulation biosynthesis. We show phosphorylated activates transcription pks2 msl3, gene(s) encoding polyketide β-ketoacyl...

Uridine-5′-diphosphoglucose (UDPG) activates the P2Y14 receptor, a neuroimmune system GPCR. receptor tolerates glucose substitution with small alkyl or aryl groups its truncation to uridine 5′-diphosphate (UDP), full agonist at human expressed in HEK-293 cells. 2-Thiouracil derivatives displayed selectivity for activation of vs P2Y6 such as 2-thio-UDP 4 (EC50 = 1.92 nM P2Y14, 224-fold P2Y6) and β-propyloxy ester 18. EC50 values β-methyl UDP 2-thio analogue were 2730 56 nM, respectively....

Abstract Organisms use circadian clocks to generate 24‐h rhythms in gene expression. However, the clock can interact with other pathways shorter period oscillations. It remains unclear how these different frequencies are generated. Here, we examine this problem by studying coupling of alternative sigma factor sigC cyanobacterium Synechococcus elongatus . Using single‐cell microscopy, find that psbAI , a key photosynthesis regulated both and clock, is activated two peaks expression every...

Summary The co‐ordinated regulation of heat shock proteins is critically important for the stress response M . tuberculosis , failure which results in enhanced immune recognition tubercle bacilli with reduced survival during chronic infections. In this study, we show that PhoP regulates transcription α‐crystallin 2 ( acr2 ), expression increases more than any other gene heat‐shock or following macrophage infection. We also by attributable to direct regulator‐promoter interactions at specific...

This study aims to evaluate the effect of core strengthening exercises on inspiratory muscle strength and functional capacity in patients with CKD undergoing Haemodialysis. The included 10 hemodialysis according eligibility criteria. All performed for 6 weeks (2 times/week). outcome measures are assessed before after intervention using capacity. We found significant improvements (p=0.150) (p=0.004) exercises. At end result, we concluded that exercise program improves

Abstract The insulin‐linked polymorphic region (ILPR) is a VNTR located upstream of the insulin ( INS ) gene consisting repeat 5′‐ACAGGGGTGTGGGG (repeat a) and several less abundant sequence repeats (b–n). Here, we have investigated structural polymorphism G‐quadruplexes formed from most common sequences (a–c) their effect on protein binding. We first established that ILPR “b” “c” can form quadruplex structures. Insulin has previously been shown to bind G‐quadruplex by dimer “a”. Our...

The P2Y14 receptor is a G protein-coupled activated by uridine-5′-diphosphoglucose and other nucleotide sugars that modulates immune function. Covalent conjugation of agonists to PAMAM (polyamidoamine) dendrimers enhanced pharmacological activity. Uridine-5′-diphosphoglucuronic acid (UDPGA) its ethylenediamine adduct were suitable functionalized congeners for coupling several generations (G2.5−6) (both terminal carboxy amino). Prosthetic groups, including biotin avidin complexation,...

The CD44 receptor is common among many cancer types where overexpression synonymous with poor prognosis in prostate, glioma, and breast cancer. More notably has been shown a number of different stem cells (CSC) which are present solid tumors drive growth, recurrence, resistance to conventional therapies. Triple negative CSCs correlate worse early relapse due higher drug increased tumor heterogeneity thus prime targets for anticancer therapy. To specifically target overexpressing receptors,...

Abstract Background Quantum dots (QDs) are crystalline nanoparticles that compatible with biological systems to provide a chemically and photochemically stable fluorescent label. New ligand probes reporter groups needed for detection characterization of G protein-coupled receptors (GPCRs). Results Synthetic strategies coupling the A 2A adenosine receptor (AR) agonist CGS21680 (2-[4-(2-carboxyethyl)phenylethylamino]-5'- N -ethylcarboxamidoadenosine) functionalized QDs were explored....

The relation between hypertension and hyperuricemia has been established by epidemiological studies. Calcium channel blockers are one of the first-line drugs for newly diagnosed patients with essential hypertension. Cilnidipine is a new calcium blocker acting blocking both L- N-type channels. aim this study was to compare effectiveness amlodipine cilnidipine in their effects on heart rate serum uric acid levels.Out 100 enrolled patients, 92 completed study. They were randomly assigned (N =...

Mycobacterium tuberculosis PhoP, a master regulator involved in complex lipid biosynthesis and expression of unknown virulence determinants, is composed an N-terminal receiver domain C-terminal effector domain. The two experimentally characterized PhoP orthologs, from Escherichia coli Salmonella enterica, display vastly different regulatory capabilities. Here, we demonstrate that the 20-residue-long arm unique to M. plays essential role expanded capabilities this important regulator....

Abstract In this work, the core carbon nanoparticles (BM CNs) and polyethylene glycol 400 (PEG )-capped BM CNs were synthesised from Butea monosperma flower extract via facile direct heating method. They investigated for their intriguing properties in vitro antioxidation activity comparison with Carica papaya seed (CPS) DPPH assay. The characterised by DLS, XRD, HR-TEM, TGA, FT-IR, UV–vis spectral analysis quantum yield measurement. PEGylated demonstrated decent yield, favourable size,...

Many epidemiological studies have established the relationship between hypertension and dyslipidemia. Calcium channel blockers (CCBs) are one of first-line drugs for newly diagnosed patients with essential hypertension. Cilnidipine as a newer CCB acting by blocking both L- N-type calcium channels possesses additional beneficial effects apart from lowering blood pressure (BP). The aim this study was to evaluate effectiveness cilnidipine in borderline dyslipidemia its on lipid profile.Out 45...

Head and neck cancer (HNC), in current days, is one of the fatal diseases. Chemotherapy main treatments for cancer. has an adverse impact on respiratory parameters patients. In these patients, expiratory force-generating capacity impaired, which usually helps patients cough effectively airway clearance. Thus, as chemotherapy, clearance affected HNC This study aimed to evaluate chemotherapy muscle strength stage 1 head We conducted observational including 15 with department oncology a...

Cellular processes are often dynamic and oscillatory, yet their coordination is essential for the proper functioning of a cell. How distinct oscillatory can couple to one another in single cell still an open question. In this study, we use cyanobacterial circadian clock as model system explore coupling pulsatile gene circuits. The generates 24-h oscillation downstream targets order time across day/night cycle. part, timing mediated by clock’s modulation activity alternative sigma factors,...

Upper abdominal surgery (UAS) alters postoperative pulmonary function, as observed by impairment of lung volumes such total capacity, vital capacity. Impaired clearance sputum results in a vicious cycle colonization and infection bronchi with pathogenic organisms, dilation further production. Ten patients undergone UAS were assigned randomly two groups equally; their ages ranged from 23 to 60 years. The group A received Buteyko breathing. data collected before after the same treatment period...