A5056908319- Receptor Mechanisms and Signaling
- Dementia and Cognitive Impairment Research
- Adenosine and Purinergic Signaling
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Parkinson's Disease Mechanisms and Treatments
- Ginkgo biloba and Cashew Applications
- Computational Drug Discovery Methods
- Ion channel regulation and function
- Pharmacological Receptor Mechanisms and Effects
- Neurological Disease Mechanisms and Treatments
- Neurotransmitter Receptor Influence on Behavior
- MicroRNA in disease regulation
- S100 Proteins and Annexins
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological disorders and treatments
- Nicotinic Acetylcholine Receptors Study
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neural Engineering
- Nuclear Receptors and Signaling
- Photoreceptor and optogenetics research
- Medication Adherence and Compliance
- Apelin-related biomedical research
- Neuropeptides and Animal Physiology
Universitat de Barcelona
2013-2025
Institut d'Investigació Biomédica de Bellvitge
2013-2025
Universitat Internacional de Catalunya
2021-2024
Fundació ACE
2022-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2014-2024
Instituto de Salud Carlos III
2024
Bellvitge University Hospital
2023
Karolinska Institutet
2020-2021
Departament de Salut
2021
University of Coimbra
2019
Abstract BACKGROUND We aimed to evaluate the precision of Alzheimer's disease (AD) and neurodegeneration biomarker measurements from venous dried plasma spots (DPS v enous ) for diagnosis monitoring neurodegenerative diseases in remote settings. METHODS In a discovery ( n = 154) validation cohort 115), glial fibrillary acidic protein (GFAP); neurofilament light (NfL); amyloid beta (Aβ) 40, Aβ42; phosphorylated tau (p‐tau181 p‐tau217) were measured paired DPS ethylenediaminetetraacetic acid...
Blood-cell-free circulating micro-RNAs (miRNAs) have been proposed as potential accessible biomarkers for neurodegenerative diseases such Parkinson's disease (PD). Here we analyzed the serum levels of 377 miRNAs in a discovery set 10 idiopathic (IPD) patients, PD patients carriers LRRK2 G2019S mutation (LRRK2 PD), and controls by using real-time quantitative PCR-based TaqMan MicroRNA arrays. We detected candidate differentially expressed miRNAs, which were further tested first validation...
Abstract G‐protein‐coupled receptors ( GPCR s) are the largest family of integral membrane with key roles in regulating signaling pathways targeted by therapeutics, but difficult to study using existing proteomics technologies due their complex biochemical features. To obtain a global view ‐mediated and identify novel components pathways, we used modified yeast two‐hybrid MYTH ) approach identified interacting partners for 48 selected full‐length human ligand‐unoccupied s native environment....
Abstract Although beta-amyloid (Aβ) and phosphorylated tau remain the preferred targets for disease-modifying treatments (DMT) against Alzheimer’s disease (AD), part of pathophysiological mechanisms cognitive impairment are related to neuroinflammation oxidative stress. In mild (MCI), a prodromal stage AD other neurodegenerative conditions, joint appearance inflammation, stress, metabolic alterations common pathways neurotoxicity neurodegeneration. The standardized extract Ginkgo biloba EGb...
Alzheimer's disease (AD) is considered a continuum, progressing from preclinical to mild cognitive impairment (MCI) as an early stage, before reaching clinically apparent dementia. Although it difficult assess the potential impairments in performance of patients with MCI due AD, this condition should be diagnosed possible so that, by means interventions, can maintain their quality life longer. Healthcare systems support primary care physicians effort identify AD and refer memory clinics...
GPR37 is an orphan G protein-coupled receptor mostly enriched in brain areas such as the cerebellum, striatum, and hippocampus. Identified a substrate of parkin, has been suggested to play role Parkinson's disease. Distributed throughout brain, function GPR37, however, remains unknown. We now provide first mapping within hippocampus, where widely expressed localized at level extrasynaptic plasma membrane dendritic spines, shafts, axon terminals. per se does not appear learning memory, since...
Abstract Objective α-Synuclein has been studied as a potential biomarker for Parkinson’s disease (PD) with no concluding results. Accordingly, there is an urgent need to find out reliable specific biomarkers PD. GPR37 orphan G protein-coupled receptor that toxically accumulates in autosomal recessive juvenile parkinsonism. Here, we investigated whether upregulated sporadic PD, and thus suitable Methods protein density mRNA expression postmortem substantia nigra (SN) from PD patients were...
Abstract G protein-coupled receptor 37 (GPR37) is an orphan associated to Parkinson’s disease (PD) neuropathology. Here, we identified GPR37 as inhibitor of adenosine A 2A (A R) cell surface expression and function in vivo . In addition, showed that R do oligomerize the striatum. Thus, a close proximity at postsynaptic level striatal synapses was observed by double-labelling post-embedding immunogold detection. Indeed, direct receptor-receptor interaction further substantiated ligation situ...
Parkinson's disease (PD) is a neurodegenerative disorder of unknown etiology. The main treatment PD consists medication with dopamine-based drugs, which palliate the symptoms but may produce adverse effects after chronic administration. Accordingly, there need to develop novel neuroprotective therapies. Several studies suggest that omega-3 polyunsaturated fatty acids (n-3 PUFA) might provide protection against brain damage. Here, we studied several experimental models PD, using striatal...
Introduction Remote monitoring technologies (RMTs) can measure cognitive and functional decline objectively at-home, offer opportunities to passively continuously, possibly improving sensitivity reducing participant burden in clinical trials. However, there is skepticism that age or impairment may render participants unable unwilling comply with complex RMT protocols. We therefore assessed the feasibility usability of a protocol all syndromic stages Alzheimer's disease healthy control...
Abstract Background FACEmemory® is the first computerized, self-administered verbal episodic memory test with voice recognition. It can be conducted under minimal supervision and contains an automatic scoring system to avoid administrator errors. Moreover, it suitable for discriminating between cognitively healthy amnestic mild cognitive impairment (MCI) individuals, associated Alzheimer’s disease (AD) cerebrospinal fluid (CSF) biomarkers. This study aimed determine whether FACEmemory...
Abstract GPR 37, also known as parkin‐associated endothelin‐like receptor (Pael‐R), is an orphan G protein‐coupled ( GPCR ) that aggregates intracellularly in a juvenile form of Parkinson's disease. However, little about the structure or function this receptor. Here, order to better understand functioning receptor, we focused on 37 C‐terminal tail, particular cystein‐enriched region. Thus, aimed reveal role these residues plasma membrane expression and function, whether presence...
Group I metabotropic glutamate (mGlu) receptors regulate hippocampal CA1 pyramidal neuron excitability via Ca 2+ wave-dependent activation of small-conductance -activated K + (SK) channels. Here, we show that mGlu 5 and SK2 channels coassemble in heterologous coexpression systems rat brain. Further, cotransfected cells or primary neurons, receptor stimulation activated apamin-sensitive SK2-mediated currents. In addition, promoted plasma membrane targeting both proteins correlated with...
The SK2 subtype of small-conductance, Ca2+-activated K+ (SK) channels regulate the spike firing frequency and modulation Ca2+ transients in Purkinje cells. To understand molecular basis by which mediate these functions, we analysed exact location densities along neuronal surface mouse cerebellar cells using highly sensitive SDS-digested freeze-fracture replica labelling (SDS-FRL) combined with quantitative analyses. Immunogold particles for were observed on post- pre-synaptic compartments...
Serotonin (5-HT) is a hormone and neurotransmitter that modulates neural activity as well wide range of other physiological processes including cardiovascular function, bowel motility, platelet aggregation. 5-HT synthesis catalyzed by tryptophan hydroxylase (TPH) which exists two distinct isoforms; TPH1 TPH2, are responsible for peripheral central 5-HT, respectively. Due to the implication in number pathologies, depression, anxiety, autism, sexual dysfunction, irritable syndrome,...
GPR37 is an orphan G protein-coupled receptor (GPCR) implicated in several neurological diseases and important physiological pathways the brain. We previously reported that its long N-terminal ectodomain undergoes constitutive metalloprotease-mediated cleavage shedding, which have been rarely described for class A GPCRs. Here, we demonstrate protease cleaves at Glu167↓Gln168 a disintegrin metalloprotease 10 (ADAM10). This was achieved by employing selective inhibition, RNAi-mediated...
Tardive dyskinesia (TD) is a serious motor side effect that may appear after long-term treatment with neuroleptics and mostly mediated by dopamine D2 receptors (D2Rs). Striatal D2R functioning be finely regulated either adenosine A2A receptor (A2AR) or angiotensin type 1 (AT1R) through putative heteromers. Here, we examined whether A2AR AT1R oligomerize in the striatum to synergistically modulate dopaminergic transmission. First, using bioluminescence resonance energy transfer, demonstrated...