A5016832578- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Diabetes Treatment and Management
- Neuropeptides and Animal Physiology
- bioluminescence and chemiluminescence research
- Neuroendocrine regulation and behavior
- Pancreatic function and diabetes
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Diabetes Management and Research
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Biochemical Analysis and Sensing Techniques
- Protein Kinase Regulation and GTPase Signaling
- Cholinesterase and Neurodegenerative Diseases
- Adipose Tissue and Metabolism
- Lymphatic System and Diseases
- Chemical Synthesis and Analysis
- 3D Printing in Biomedical Research
- Hemoglobin structure and function
- Glaucoma and retinal disorders
- Erythrocyte Function and Pathophysiology
- Diabetes Management and Education
- Modular Robots and Swarm Intelligence
- Computational Drug Discovery Methods
Kyorin University
2020-2025
Inserm
2016-2025
Université Paris Cité
2016-2025
Institut Cochin
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Délégation Paris 5
2017-2025
Sorbonne Paris Cité
2017-2025
Imperial College London
2021
University of Birmingham
2021
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2021
The seven-spanning calcium-sensing receptor (CaSR) activates multiple G proteins including Gq and Gi, thereby a variety of second messengers inhibits parathyroid hormone (PTH) secretion. However, the exact signaling mechanisms underlying functional activity CaSR are not yet fully understood. heterozygous inactivation or its inhibition by antibody blocking results in either familial hypocalciuric hypercalcemia acquired (AHH), respectively. Here, we report identification unique autoantibody an...
Abstract G‐protein‐coupled receptors ( GPCR s) are the largest family of integral membrane with key roles in regulating signaling pathways targeted by therapeutics, but difficult to study using existing proteomics technologies due their complex biochemical features. To obtain a global view ‐mediated and identify novel components pathways, we used modified yeast two‐hybrid MYTH ) approach identified interacting partners for 48 selected full‐length human ligand‐unoccupied s native environment....
Receptors for the peptide hormones glucagon-like peptide-1 (GLP-1R), glucose-dependent insulinotropic polypeptide (GIPR), and glucagon (GCGR) are important regulators of insulin secretion energy metabolism. GLP-1R agonists have been successfully deployed treatment type 2 diabetes, but it has suggested that their efficacy is limited by target receptor desensitization downregulation due to recruitment β-arrestins. Indeed, recently described with reduced β-arrestin-2 delivered promising results...
Intraocular pressure (IOP), a key factor in glaucoma development, is regulated by aqueous humor (AH) dynamics, with inflow from the ciliary body and outflow through trabecular meshwork (TM). IOP has circadian rhythm entrained sympathetic norepinephrine (NE) superior cervical ganglion. Herein, we investigated its underlying regulatory mechanisms TM. Through comprehensive gene expression analysis of human TM cells mouse eyes, identified 18 genes upregulated NE stimulation, including small...
β-Arrestins are critical regulators of G protein-coupled receptors (GPCRs) that desensitize protein signaling, promote receptor internalization, and initiate signaling on their own. Recent structural findings indicate β-arrestins adopt different conformations upon interaction with agonist-activated GPCRs. Here, we established a β-arrestin-2 conformational bioluminescence resonance energy transfer (BRET) sensor composed the bright Nanoluc BRET donor red-shifted CyOFP1 acceptor. The monitors...
Soft robotics, a research field wherein robots are fabricated from compliant materials, has sparked widespread interest because of its potential applications in variety scenarios. In soft robots, luminescence is an important functionality for communication and information transmission, it typically achieved through electroluminescence, which relies on synthetic substances activated by external electric sources, such as batteries. This paper focuses the use luciferase, biologically derived...
Understanding the function of orphan G protein-coupled receptors (GPCRs), whose cognate ligand is unknown, major importance as GPCRs are privileged drug targets for many diseases. Recent phylogenetic studies classified three receptors, GPR61, GPR62 and GPR135 among melatonin receptor subfamily, but their capacity to bind biochemical functions not well characterized yet. We show here that do [3H]-melatonin nor 2-[125I]iodomelatonin respond in several signaling assays. In contrast, extensive...
The glucagon-like peptide-1 receptor (GLP-1R) is a class B G protein–coupled and mainstay therapeutic target for the treatment of type 2 diabetes obesity. Recent reports have highlighted how biased agonism at GLP-1R affects sustained glucose-stimulated insulin secretion through avoidance desensitization downregulation. A number agonists (GLP-1RAs) feature fatty acid moiety to prolong their pharmacokinetics via increased albumin binding, but potential these chemical changes influence function...
Aggregates of the tau protein are a well-known hallmark several neurodegenerative diseases, collectively referred to as tauopathies, including frontal temporal dementia and Alzheimer's disease (AD). Monitoring transformation process from physiological monomers into pathological oligomers or aggregates in high-throughput, quantitative manner cellular context is still major challenge field. Identifying molecules able interfere with those processes high therapeutic interest. Here, we developed...
Intracellular accumulation of tau protein is a hallmark Alzheimer’s Disease and Progressive Supranuclear Palsy, as well other neurodegenerative disorders collectively known tauopathies. Despite our increasing understanding the mechanisms leading to initiation progression pathology, field still lacks appropriate disease models facilitate drug discovery. Here, we established novel modulatable seeding-based neuronal model full-length 4R using humanized mouse cortical neurons seeds from P301S...
Abstract The glucagon-like peptide-1 receptor (GLP-1R) is a class B G protein-coupled and mainstay therapeutic target for the treatment of type 2 diabetes obesity. Recent reports have highlighted how biased agonism at GLP-1R affects sustained glucose-stimulated insulin secretion through avoidance desensitisation downregulation. A number agonists (GLP-1RAs) feature fatty acid moiety to promote albumin binding in order prolong their pharmacokinetics, but potential these ligand changes...
The contrast medium was observed as a thin line indicating active bleeding in the cellular component and stayed at boundary between plasma components liquefied hematomas. We could also reproduce same phenomenon an experimental study using human blood. characteristic dynamic motion of demonstrated hematoma, which we have called "signal flare" phenomenon, is significant sign bleeding.
Abstract Receptors for the peptide hormones glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP) and glucagon (GCG) are important regulators of insulin secretion energy metabolism. Recently described GLP-1 receptor agonists showing signal bias in favour cyclic AMP over β-arrestin-2 recruitment have delivered promising results preclinical studies. Here we first sought to establish role β-arrestins control intracellular signalling trafficking responses at closely...