A5064570527- Phytochemicals and Antioxidant Activities
- Metabolomics and Mass Spectrometry Studies
- Tea Polyphenols and Effects
- Analytical Chemistry and Chromatography
- Fermentation and Sensory Analysis
- Antioxidant Activity and Oxidative Stress
- Essential Oils and Antimicrobial Activity
- Mass Spectrometry Techniques and Applications
- Phytochemistry and Biological Activities
- Fatty Acid Research and Health
- Analytical chemistry methods development
- Advanced Chemical Sensor Technologies
- Diet and metabolism studies
- Edible Oils Quality and Analysis
- Biochemical Analysis and Sensing Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Plant Stress Responses and Tolerance
- Hops Chemistry and Applications
- Eicosanoids and Hypertension Pharmacology
- Food Chemistry and Fat Analysis
- Liver Disease Diagnosis and Treatment
- Nuts composition and effects
- Plant biochemistry and biosynthesis
- Natural product bioactivities and synthesis
- Sirtuins and Resveratrol in Medicine
Universitat de Barcelona
2014-2023
Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable
2019-2021
Instituto de Salud Carlos III
2019-2021
FC Barcelona
2015
Instituto de Gestión de la Innovación y del Conocimiento
2009-2012
Instituto de Fermentaciones Industriales
2009
Scientific Services
2006
University of Vienna
2005
Clínica Diagonal
1997
AbstractResearch has shown that fruits and vegetables containing high levels of polyphenolics (flavonoids) display total antioxidant activity. Our laboratory found various fruit vegetable extracts, particularly blueberry (BB), were effective in reversing age-related deficits neuronal signaling behavioral parameters following 8 weeks feeding, possibly due to their polyphenolic content. However, it was unclear if these phytonutrients able directly access the brain from dietary BB...
Liquid chromatography coupled with ionspray mass spectrometry in the tandem mode (LC/MS/MS) negative ion detection was used for identification of a variety phenolic compounds cocoa sample. Gradient elution water and acetonitrile, both containing 0.1% HCOOH, used. Standard solutions 31 compounds, including benzoic cinnamic acids flavonoid were studied using MS/MS product scans. At low collisional activation, deprotonated molecule [M - H](-) observed all studied. For acids, losses CO(2) or...
Abstract Pseudomonas aeruginosa 47T2, grown in submerged culture with waste frying oil as a carbon source, produced mixture of rhamnolipids surface activity. Up to 11 rhamnolipid homologs (Rha‐Rha‐C 8 ‐C 10 ; Rha‐C /Rha‐C ;Rha‐Rha‐C 12:1 Rha‐Rha‐C 12 /Rha‐Rha‐C 14:1 ) were isolated from cultures P. 47T2 and identified by HPLC‐MS analysis. This article deals the production, isolation, chemical characterization RL . The physicochemical biological properties new product also studied. Its...
Liquid chromatography (LC) diode array detection (DAD) coupled to negative electrospray ionization (ESI) tandem mass spectrometry (MS/MS) was used for the rapid and sensitive identification of water-soluble phenolic compounds in fennel waste. The plant material first extracted then chromatographed on Sephadex LH-20 afford seven fractions, each them being subjected LC-MS analysis. Identification carried out by interpretation UV, MS, MS/MS spectra. Forty-two substances were identified, 27...
A simple and reproducible method for qualitative quantitative analysis of phenolic compounds in virgin olive oils by solid-phase extraction (SPE), high performance liquid chromatography with diode array detector (HPLC-DAD), HPLC-mass spectrometry (MS) tandem mode was developed. The polar fraction obtained from samples three different oils. Detection quantification were performed at 280, 240, 320 nm. For identification purposes, HPLC-MS/MS equipped turbo ion spray source the negative-ion...
Abstract Tomato ( Lycopersicon esculentum Mill.) is the second most important fruit crop worldwide. Tomatoes are a key component in Mediterranean diet, which strongly associated with reduced risk of chronic degenerative diseases. In this work, we use combination mass spectrometry (MS) techniques negative ion detection, liquid chromatography/electrospray ionization linear trap quadrupole‐Orbitrap‐mass (LC/ESI‐LTQ‐Orbitrap‐MS) and tandem (LC/ESI‐MS/MS) on triple quadrupole, for identification...
Cocoa-phytochemicals have been related to the health-benefits of cocoa consumption. Metabolomics has proposed as a powerful tool characterize both intake and effects on metabolism dietary components. Human urine metabolome modifications after single were explored in randomized, crossed, controlled trial. After overnight fasting, 10 subjects consumed randomly either dose powder with milk or water, without cocoa. Urine samples collected before ingestion at 0−6, 6−12, 12−24-h test-meals Samples...
The exposome, defined as the cumulative measure of external exposures and associated biological responses throughout lifespan, has emerged in recent years a cornerstone biomedical sciences. Metabolomics stands out here one most powerful tools for investigating interplay between genetic background, exogenous, endogenous factors within human health. However, to address complexity novel methods are needed characterize metabolome. In this work, we have optimized validated multianalyte...
The occurrence of phenolic compounds in apple residues resulting from the juice industry was investigated to provide an alternative use for this raw material. For identification these compounds, liquid chromatography coupled ionspray mass spectrometry tandem mode (LC/MS/MS) with negative ion detection used. were first extracted and then chromatographed on Sephadex LH-20 yield 13 fractions. Positive based their retention times spectra full scan (MS), different MS/MS modes (product scan,...
Presented in this paper is a new method for the identification of tartaric acid as wine marker archaeological residues from Egyptian vessels using liquid chromatography with mass spectrometry tandem mode (LC/MS/MS). Owing to special characteristics these samples, such dryness and small quantity available analysis, it was necessary have very sensitive highly specific analytical detect at trace levels residues. Furthermore, an alkaline fusion carried out identify syringic derived malvidin red...
In this paper, a sensitive, precise, and selective analytical method has been developed for the identification quantification of resveratrol metabolites in human low-density lipoprotein (LDL) after moderate consumption red wine, using high-performance liquid chromatography electrospray tandem mass spectrometry (LC−ESI-MS/MS). From different extraction procedures tested, solid-phase was selected to minimize matrix effects reaching highest sensitivity. Standard calibration curves prepared LDL...
Tomatoes (Solanum lycopersicum L.), the second most important vegetable crop worldwide, are a key component in so-called "Mediterranean diet", which is strongly associated with reduced risk of chronic degenerative diseases. In this work, we evaluate differences total and individual polyphenol content hydrophilic antioxidant capacity seven varieties tomato cultivated Vegas Bajas del Guadiana, Badajoz (Spain), were collected from two consecutive harvests (2008-2009). Hydrophilic was evaluated...
Through an HPLC-Q-TOF-MS-driven nontargeted metabolomics approach, we aimed to discriminate changes in the urinary metabolome of subjects with metabolic syndrome (MetS), following 12 weeks mixed nuts consumption (30 g/day), compared sex- and age-matched individuals given a control diet. The corresponding nut-enriched diet clearly clustered distinct group, multivariate data analysis discriminated relevant mass features this separation. Metabolites discriminating ions (MS features) were then...
The present study aims to compare the phenolic profiles of organic and conventional tomatoes bought in market. For quantification identification individual polyphenols, liquid chromatography coupled mass spectrometry tandem mode (LC-MS/MS) was carried out. Confirmation compounds previously identified on triple-quadrupole accomplished by injection high-resolution system (QToF-MS). In this way, 34 were tomato fruits. Recoveries targeted polyphenols exceed 78% for tomatoes, respectively. method...
Scope Tomato contains a variety of phenolics associated with health‐promoting properties. However, the effects processing and addition oil during tomato sauce preparation on microbial metabolism in small intestine are still unclear. Methods results An open, controlled, randomized, crossover feeding trial 40 healthy volunteers was carried out to analyze metabolites plasma urine after consumption sauces, enriched refined olive (ROOE) without (oil‐free: OF). Ten 93 were quantified. Processing...
Abstract Background: Nutritional biomarkers are alternatives to traditional dietary assessment tools. We sought develop a method for nutritional analysis of resveratrol, phenolic compound with purported health-promoting properties, and determine all resveratrol metabolites. Methods: obtained LDL urine samples from 11 healthy male volunteers who had consumed 250 mL Merlot red wine. measured its metabolites 96-well solid-phase extraction plates coupled HPLC-tandem mass spectrometry. Hexestrol...