Fat mass is a predictor of BMD; however, the mechanisms involved remain uncertain. Two adipokines, leptin and adiponectin, were examined as potential mediators this relation in 80 perimenopausal women. Adiponectin did not exert any effect on BMD, whereas exerted negative one, with insulin acting confounder to relation.Fat an important determinant bone density, but mechanism Leptin circulating peptides adipocyte origin, are contributors relation. We investigated role adiponectin mediating fat...

Purpose: To describe the pharmacokinetics of intravitreal bevacizumab (Avastin ® ) in rabbits. Methods: The right eye 20 rabbits was injected intravitreally with 1.25 mg/0.05 mL bevacizumab. Both eyes four each time were enucleated at days 1, 3, 8, 15, and 29. Bevacizumab concentrations measured serum, aqueous humor, vitreous. Results: Maximum vitreous (406.25 µg/mL) humor (5.83 day 1. Serum concentration peaked 8 (0.413 declined to 0.032 µg/mL 4 weeks. Half-life values vitreous, serum 6.61,...

The most widely used test for the diagnosis of SARS-CoV-2 infection is a PCR test. has very high sensitivity and able to detect low amounts RNA. However, many individuals receiving positive result in context PCR-based surveillance might be infected with SARS-CoV-2, but they are not contagious at time question arises regards if cost effective, portable rapid antigen tests (RATs) have better performance than identification infectious individuals. In this direction, we examined diagnostic RATs...

The prevalence of peripheral neuropathy in patients with Sjögren syndrome remains unclear owing to conflicting results the published series, numbers ranging from 2% over 60% patients. Whether is a feature systemic or glandular disease whether it related circulating antineuronal antibody also uncertain.The authors reviewed records primary (pSS), fulfilling Revised European-American Classification Criteria, seen their department 1992 2009. previously recorded neuropathic features were...

Summary Coxsackie virus RNA has recently been detected in biopsy specimens of minor salivary glands from patients with primary Sjögren's Syndrome (pSS). A peptide derived 2B protein (pepCoxs) presents 87% sequence homology the 222–229 region major linear B-cell epitope Ro60 kD autoantigen (pep216–232). Synthetic peptides corresponding to pep216–232: 216KALSVETEKLLKYLEAV232 and pepCoxs: 31MVTSTITEKL LKNLVKI47, were prepared. Sera 42 pSS 43 systemic lupus erythematosus (SLE) as well sera 27...

Congenital heart block (CHB), a manifestation of neonatal lupus, is associated with maternal anti-Ro/SSA and anti-La/SSB autoantibodies recurs in ∼18% subsequent pregnancies. This study was undertaken to investigate the effect idiotype:antiidiotype (Id:anti-Id) antibody ratio ability intravenous immunoglobulin (IVIG) administered during pregnancies prevent CHB.We studied 16 anti-La/SSB-positive pregnant women from Preventive IVIG Therapy for Heart Block who had previously given birth child...

Circulating autoantibodies to endogenous erythropoietin (anti-Epo) are detected in human immunodeficiency virus type 1 (HIV-1)-infected patients and represent a risk factor for anemia. The aim of this study was map the B-cell epitopes on Epo molecule.Serum samples from HIV-1-positive healthy individuals were tested against overlapping peptides covering entire sequence Epo.Serum anti-Epo-positive exhibited significant binding spanning following sequences: amino acids 1-20 (Ep1), 54-72 (Ep5),...

SUMMARY The B cell epitope mapping of La/SSB was performed using 20 mer synthetic peptides overlapping by eight amino acids covering the whole sequence protein. IgG, purified from sera five patients with systemic lupus erythematosus (SLE) and four primary Sjögren's syndrome (pSS) were tested against peptides. Peptides highly reactive IgG those spanning regions 145–164, 289–308, 301–320 349–368 La Determination minimum required length antigenic determinants disclosed following...

Calreticulin is a molecular chaperone to newly synthesized polypeptides. Previous studies suggested that calreticulin probably protein member of the Ro/La RNP complex. The aims this study were (a) investigate whether linear B cell epitopes complex are bound and (b) if peptide-calreticulin recognized specifically by anti-Ro autoantibodies. was isolated from either human or pig spleen using multi-step purification method found interact preferentially with biotinylated peptides derived sequence...

The association of celiac disease (CD) with HLA-DQ2 and HLA-DQ8 is indicative preferential mucosal T cell recognition gluten fragments bound to either DQ allele. We have recently identified two gluten-derived, HLA-DQ8-restricted stimulatory peptides, one each from gliadin glutenin, recognized by specific clones derived the small intestine CD patients. now performed molecular modeling examined fine specificity these peptides in complex HLA-DQ8. There only binding register for both glutamine...

Autoantigen La/SSB is molecular target of humoral autoimmunity in patients with primary Sjogren's Syndrome (pSS) and systemic lupus erythematosus (SLE). In this study, we investigated the existence possible influence anti-idiotypic response to anti-La/SSB antibodies. Synthetic peptide analogs (pep) major antigenic determinants (289–308aa 349–364aa) were prepared. Based on "molecular recognition" theory, complementary peptides (cpep), derived by anti-parallel readings noncoding strand DNA...

Summary Immunologically mediated thrombocytopenia is a frequent clinical manifestation in patients with systemic lupus erythematosus (SLE). Autoantibodies targeting platelet membrane glucoproteins have central role peripheral destruction. against thrombopoietin are also present about one-third of patients, but their pathogenetic obscure. Thirty-eight serum samples from SLE were tested for anti-platelet antibodies, anti-thrombopoietin antibodies and levels circulating thrombopoietin. Bone...