A5048306885- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Cavitation Phenomena
- Ultrasound Imaging and Elastography
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Photodynamic Therapy Research Studies
- Advanced Optical Sensing Technologies
- Fluid Dynamics and Mixing
- Cardiovascular Health and Disease Prevention
- Abdominal vascular conditions and treatments
- Pluripotent Stem Cells Research
- Vascular anomalies and interventions
- Ocular and Laser Science Research
- Optical Systems and Laser Technology
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Viral gastroenteritis research and epidemiology
- Image Processing Techniques and Applications
- Membrane-based Ion Separation Techniques
- Lattice Boltzmann Simulation Studies
- Microfluidic and Bio-sensing Technologies
- Thermal Regulation in Medicine
- Cardiovascular and Diving-Related Complications
Concordia University
2020-2025
University of Pittsburgh Medical Center
2015-2024
University of Toronto
2012-2018
Sunnybrook Health Science Centre
2010-2018
Sunnybrook Research Institute
2012-2018
University of Pittsburgh
2015-2017
Sunnybrook Hospital
2012-2014
Health Sciences Centre
2011-2012
Significance Gas-filled microbubbles physically oscillate in an ultrasound field and have been shown to potentiate the delivery of therapeutic payloads. The biophysical mechanisms by which vibrating stimulate macromolecule uptake across cell membrane, however, remain unknown. With a coupled microscopy system capable bright-field imaging up 25 million frames per second, we show correlations between microsecond-scale bubble oscillations second-to-minute–scale diffusion, uniquely highlighting...
Porphyrin-phospholipid conjugates were used to create photonic microbubbles (MBs) having a porphyrin shell ("porshe"), and their acoustic photoacoustic properties investigated. The inclusion of porphyrin-lipid in the MB increased yield, improved serum stability, generated narrow volumetric size distribution with peak 2.7 ± 0.2 μm. Using an model, we calculated porshe stiffness be 3-5 times greater than that commercial lipid MBs. Porshe MBs found intrinsically suitable for both ultrasound...
Ultrasound and photoacoustic imaging are highly complementary modalities since both use ultrasonic detection for operation. Increasingly, ultrasound have been integrated in terms of hardware instrumentation. To generate a broadly accessible dual-modality contrast agent, we generated microbubbles (a standard agent) solution methylene blue dye). This MB 2 was formed effectively optimized as solution. As microbubble concentration increased (with constant), signal attenuated the When held no...
The development of microbubble contrast agents has broadened the scope medical ultrasound imaging. Along with dedicated imaging techniques, these provide enhanced echoes from blood pool and have enabled diagnostic to assess quantify microvascular flow. Contrast-enhanced is currently used worldwide clinical indications in cardiology radiology, it continues evolve develop through innovative technological advancements. In this review article, we present an overview basic physics bubble-specific...
With the widespread interest and uptake of super-resolution ultrasound (SRUS) through localization tracking microbubbles, also known as microscopy (ULM), many algorithms have been developed. ULM can image centimeters into tissue in-vivo track microvascular flow non-invasively with sub-diffraction resolution. In a significant community effort, we organized challenge, Ultrasound Localization TRacking Algorithms for Super-Resolution (ULTRA-SR). The aims this paper are threefold: to describe...
Phospholipid encapsulated ultrasound contrast agents have proven to be a powerful addition in diagnostic imaging and show emerging applications targeted therapy due their resonant nonlinear scattering. Microbubble response is affected by intrinsic (e.g. bubble size, encapsulation physics) extrinsic boundaries) factors. One of the major factors at play affecting microbubble vibration dynamics initial phospholipid packing lipid encapsulation. Here, we examine how affects subharmonic either...
The recent and exciting success of anti-inflammatory therapies for ischemic heart disease (e.g. atherosclerosis) is hindered by the lack site-specific targeted therapeutic deposition. Microbubble-mediated focused ultrasound, which uses circulating, lipid-encapsulated intravascular microbubbles to locally enhance endothelial permeability, offers an approach. Atherosclerotic plaques preferentially develop in regions with disturbed blood flow, microbubble-endothelial cell membrane interactions...
There is a growing interest in microbubble based ultrasound contrast imaging applications the 5-15 MHz range. In this study, individual microbubbles were insonified at low pressures (≤ 25 kPa) using an "acoustic spectroscopy" approach which entailed transmitting sequence of tone bursts with center frequencies ranging from 4 to 13.5 MHz. The fundamental (transmit) frequency radial excursion amplitude was calculated scattered signals produce resonance curve for each bubble. For diameters...
Medical ultrasound is one of the most widely used imaging modalities worldwide. Microbubbles, typically ~1–8 μm in diameter, are contrast agents confined to vasculature due their size. Microbubbles have broadened scope medical ultrasound, permitting real-time microvasculature for blood flow assessment, molecular imaging, and even non-invasive site-specific therapy. Recently, there has been increasing interest developing submicron, “nanoscale” extend utility ultrasound. In this review, we...
Ultrasound-driven microbubbles, typically between 1 and 8 µm in diameter, are resonant scatterers that employed as diagnostic contrast agents emerging potentiators of targeted therapies. Microbubbles administered populations whereby their radial dynamics – key to effectiveness - greatly affected by intrinsic (e.g. bubble size) extrinsic boundaries) factors. In this work, we aim understand how two neighbouring microbubbles influence each other. We developed a finite element model system...
Over the past two decades, there has been tremendous and exciting progress toward extending use of medical ultrasound beyond a traditional imaging tool. Ultrasound contrast agents, typically used for improved visualization blood flow, have explored as novel non-viral gene delivery vectors cardiovascular therapy. Given this adaptation to contrast-enhancing presents an image-guided site-specific technique with potential multi-gene repeatable protocols—overcoming some limitations alternative...
Cellular immunotherapy remains hindered in the context of solid tumors due to immunosuppressive microenvironment, which key endothelial cell adhesion molecules (CAM) are suppressed. Microbubble‐mediated focused ultrasound is being explored for targeted and can exert local shear stress upon neighboring cells. However, fluid microbubble‐induced modulation immunobiology not well understood. Herein, influence both types on human vein (HUVEC) brain (HBEC‐5i) CAM expression secretion over 90...
The effect of boundary proximity on ultrasound contrast agent microbubble emissions can play an important role in the context both targeted imaging and microvascular perfusion. In this study, individual microbubbles (n = 104) were insonicated as a function distance from either polystyrene membrane (OpticellTM) or compliant agarose up to offset distances 1000 µm by use optical trapping setup. An 'acoustic spectroscopy' approach was employed, which entailed transmitting sequence tone bursts...