A5102719837- Aquaculture disease management and microbiota
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Invertebrate Immune Response Mechanisms
- interferon and immune responses
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- RNA regulation and disease
- Escherichia coli research studies
- Viral Infections and Vectors
- Antimicrobial Peptides and Activities
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Proteoglycans and glycosaminoglycans research
- Amphibian and Reptile Biology
- Autism Spectrum Disorder Research
- Carbohydrate Chemistry and Synthesis
- Cellular transport and secretion
- Immune responses and vaccinations
- Infectious Diseases and Mycology
- Actinomycetales infections and treatment
- Respiratory viral infections research
Northeast Agricultural University
2016-2022
Peking University
2021
Guangxi University
2021
Nantong University
2021
University of Nebraska Medical Center
2020
Porcine epidemic diarrhea caused by porcine virus (PEDV) has led to serious economic losses the swine industry worldwide. In this study, an oral recombinant Lactobacillus casei vaccine against PEDV infection targeting intestinal microfold (M) cells and dendritic (DCs) for delivering core neutralizing epitope (COE) of spike protein was developed with M cell-targeting peptide (Col) (DCpep). The immunogenicity orally administered strains evaluated. After immunization, significantly higher...
Porcine epidemic diarrhea virus (PEDV), an enteric coronavirus, is the causative agent of porcine (PED) that damages intestinal epithelial cells and results in severe dehydration neonatal suckling pigs with up to 100% mortality. The oral vaccine route reported as a promising approach for inducing protective immunity against PEDV invasion. Furthermore, dendritic (DCs), professional antigen-presenting cells, link humoral cellular immune responses homeostasis environment. In this study, order...
The aims of this study were to develop an effective oral vaccine against enterotoxigenic Escherichia coli (ETEC) infection and design new more versatile mucosal adjuvants.Genetically engineered Lactobacillus casei strains expressing F4 (K88) fimbrial adhesin FaeG (rLpPG-2-FaeG) either co-expressing heat-labile enterotoxin A (LTA) subunit with amino acid mutation associated reduced virulence (LTAK63) a B (LTB) E. (rLpPG-2-LTAK63-co-LTB) or fused-expressing LTAK63 LTB (rLpPG-2-LTAK63-fu-LTB)...
The aims of this study were to develop an effective M cell‐targeting oral vaccine, involving Lactobacillus casei deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with peptide Co1 as adjuvant, against PEDV infection. Genetically engineered L. 393 (L393) strains expressing COE only (pPG‐COE/L393) or fused‐expressing and (pPG‐COE‐Co1/L393) constructed, immunogenicity upon administration vaccine was evaluated. results showed that higher...
Type II transmembrane serine proteases (TTSPs) facilitate the spread and replication of viruses such as influenza human coronaviruses, although it remains unclear whether TTSPs play a role in progression animal coronavirus infections, that by porcine epidemic diarrhea virus (PEDV). In this study, including TMPRSS2, HAT, DESC1, MSPL were tested for their ability to PEDV Vero cells. Our results showed TMPRSS2 played significant roles stages cell–cell fusion virus–cell fusion, whereas HAT DESC1...
Porcine epidemic diarrhea virus (PEDV), which is the causative agent of porcine in China and other countries, responsible for serious economic losses pork industry. Inactivated PEDV vaccine plays a key role controlling prevalence PEDV. However, consistently low viral titers are obtained during propagation vitro; this represents challenge to molecular analyses development. In study, we successfully isolated isolate (strain NJ) from clinical samples collected recent outbreak piglets China,...
IHNV is a virus that infects salmonids and causes serious economic damage to the salmonid farming industry. There no specific treatment for disease caused by this pathogen main preventive measure vaccination, but only possible small groups of individuals. Therefore, it important investigate new oral vaccines prevent IHNV. In study, CK6 chemokine protein rainbow trout truncated G were used construct secretory expression recombinant L.casei vaccine trout. The results showed levels IgM IgT...
To develop an effective oral vaccine against the very virulent infectious bursal disease virus (vvIBDV), we generated two recombinant Lactobacillus plantarum strains (pPG612‐VP2/LP and pPG612‐T7g10‐VP2/LP, which carried T7g10 translational enhancer) that displayed VP2 protein on surface, compared humoral cellular immune responses vvIBDV in chickens. We genetically engineered L. pPG612‐VP2/LP pPG612‐T7g10‐VP2/LP constitutively expressing of vvIBDV. found enhancer efficiently upregulates...
Abstract Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic (IPNV) are important pathogens in rainbow trout farming worldwide. Their co‐infection is also common, which causes great economic loss juvenile salmon species. Development of a universal vaccine providing broadly cross‐protective immunity will be importance. In this study, we generated two recombinant (r) (rIHNV‐N438A‐ΔNV‐EGFP rIHNV‐N438A‐ΔNV‐VP2) replacing the NV gene backbone rIHNV at single point mutation...
Avian colibacillosis is responsible for economic losses to poultry producers worldwide. To combat this, we aimed develop an effective oral vaccine chicken against O78 avian pathogenic Escherichia coli (APEC) infection through a Lactobacillus delivery system.Eight strains isolated from the intestines of broiler chickens were evaluated based on their in vitro adherence ability assess potential as vector. Fimbrial subunit A (FimA) and outer-membrane protein C (OmpC) APEC with without fusion...
Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine diarrhea, causing substantial economic losses to swine industry worldwide. However, development PEDV vaccine hampered by its low propagation titer in vitro, due difficulty adapting cells and complex culture conditions, even presence trypsin. Furthermore, frequent variation, recombination, evolution resulted reemergence vaccination failure. In this study, we established Vero/TMPRSS2 Vero/MSPL cell lines, constitutively...
Salmonid alphaviruses (SAV) severely infect farmed salmonids and rainbow trout. Owing to the recent increase in fish import trade, several SAV unreported countries, such as China, may face serious threat of this infection. Thus, it is necessary develop efficient detection methods for prevention diagnosis In study, we selected a conserved segment E1 protein target immunogen preparation monoclonal antibodies (mAbs) specific SAV. A sensitive indirect immunofluorescence (IF) method was developed...