A5100336118- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Probiotics and Fermented Foods
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Immunotherapy and Immune Responses
- Transgenic Plants and Applications
- Aquaculture disease management and microbiota
- Antimicrobial Peptides and Activities
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- Herpesvirus Infections and Treatments
- Milk Quality and Mastitis in Dairy Cows
- Escherichia coli research studies
- interferon and immune responses
- Infant Nutrition and Health
- Bacteriophages and microbial interactions
- Food Safety and Hygiene
- Microencapsulation and Drying Processes
- Monoclonal and Polyclonal Antibodies Research
- ATP Synthase and ATPases Research
- Cholesterol and Lipid Metabolism
- vaccines and immunoinformatics approaches
Northeast Agricultural University
2010-2025
Hebei Normal University of Science and Technology
2024
Guangdong Academy of Agricultural Sciences
2022
Xizang Minzu University
2021
Peking Union Medical College Hospital
2021
Peking University
2021
Sun Yat-sen University
2021
Sichuan University
2021
Shanghai Jiao Tong University
2021
Central South University
2021
Abstract Background Porcine epidemic diarrhea (PED) is an infectious disease of the digestive tract caused by porcine virus (PEDV), characterized vomiting, severe diarrhea, and high mortality rates in piglets. In recent years, distribution this China has remarkably increased, its pathogenicity also increased. PEDV been identified as main cause viral This study aimed to understand genetic evolution diversity provide a theoretical basis for development new vaccines prevention treatment PED....
Porcine epidemic diarrhea caused by porcine virus (PEDV) has led to serious economic losses the swine industry worldwide. In this study, an oral recombinant Lactobacillus casei vaccine against PEDV infection targeting intestinal microfold (M) cells and dendritic (DCs) for delivering core neutralizing epitope (COE) of spike protein was developed with M cell-targeting peptide (Col) (DCpep). The immunogenicity orally administered strains evaluated. After immunization, significantly higher...
MicroRNAs (miRNAs) play an important role in the regulation of immune responses. Previous studies have indicated that dysregulating miRNAs leads to immunosuppression porcine reproductive and respiratory syndrome virus (PRRSV). However, it is not clear how PRRSV regulates expression host miRNA, which may lead escape or promote replication virus. The present work suggests upregulated miR-373 through elevating specificity protein 1 (Sp1) MARC-145 cells. Furthermore, this demonstrated promoted...
Porcine epidemic diarrhea virus (PEDV), an enteric coronavirus, is the causative agent of porcine (PED) that damages intestinal epithelial cells and results in severe dehydration neonatal suckling pigs with up to 100% mortality. The oral vaccine route reported as a promising approach for inducing protective immunity against PEDV invasion. Furthermore, dendritic (DCs), professional antigen-presenting cells, link humoral cellular immune responses homeostasis environment. In this study, order...
Abstract Porcine epidemic diarrhea (PED) caused by porcine virus (PEDV), is an acute and highly infectious disease, resulting in substantial economic losses the pig industry. Given that PEDV primarily infects mucosal surfaces of intestinal tract, it crucial to improve immunity prevent viral invasion. Lactic acid bacteria (LAB) oral vaccines offer unique advantages potential applications combatting diseases, making them ideal approach for controlling PED outbreaks. However, traditional LAB...
The primary aim of this study was to evaluate the efficacy phage against mastitis induced by drug-resistant S. aureus in a mouse model. In study, five phages—4086-1, 4086-2, 4086-3, 4086-4, and 4086-6—were isolated from milk samples secreted cows. Transmission electron microscopy showed that all phages had icosahedral heads short non-contractile tails, which are typical characteristics family Podoviridae. All these were species-specific aureus. one-step growth curve latency period (10–20...
An oral probiotic vaccine, pYG-KHV-ORF81/LR CIQ249, encapsulated by chitosan-alginate capsules as an delivery system was developed for koi carp against herpesvirus (KHV) infection. This vaccine can be protected from various digestive environments and maintain effectively high viability, showing a good tolerance to environments.
<title>Abstract</title> Local mucosal immune responses play a crucial role in protecting surfaces from infections, with the mucus layer serving as significant component of barrier that prevents direct contact foreign materials epithelial cells. Research has focused on using Lactic acid bacteria (LAB) potential carriers for oral vaccines due to their ability colonize intestine and stimulate high levels antibodies against expressed antigens. However, mechanism interaction between LAB vector...
Porcine transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea (PEDV) are the causative agents of highly fatal acute in pigs, resulting enormous losses pig industry worldwide. To develop an effective bivalent oral vaccine against TGEV PEDV infection, D antigenic site spike (S) protein major antigen (core neutralizing epitope-COE) S were used as immunogens, enhanced green fluorescent (eGFP) gene was a reporter to construct genetically engineered Lactobacillus casei...
Transmissible gastroenteritis virus (TGEV) infection causes acute enteritis in swine of all ages, and especially suckling piglets. Small intestinal inflammation is considered a central event the pathogenesis TGEV infections, nuclear factor-kappa B (NF-κB) key transcription factor inflammatory response. However, it unclear whether NF-κB crucial for inducing during infection. Our results show that was activated testicular (ST) cells epithelial cell lines J2 (IPEC-J2) infected with TGEV, which...
Dendritic cells (DCs) present an ideal target for delivering immunogenic cargo due to their potent antigen-presenting capabilities. This targeting approach holds promise in vaccine development by enhancing the efficiency of antigen recognition and capture DCs. To identify a high-affinity peptide binding rabbit DCs, monocyte-derived DCs (raMoDCs) were isolated cultured, novel peptide, HS (HSLRHDYGYPGH), was identified using phage-displayed library. Alongside HS, two other DC-targeting...
Type II transmembrane serine proteases (TTSPs) facilitate the spread and replication of viruses such as influenza human coronaviruses, although it remains unclear whether TTSPs play a role in progression animal coronavirus infections, that by porcine epidemic diarrhea virus (PEDV). In this study, including TMPRSS2, HAT, DESC1, MSPL were tested for their ability to PEDV Vero cells. Our results showed TMPRSS2 played significant roles stages cell–cell fusion virus–cell fusion, whereas HAT DESC1...
Porcine epidemic diarrhea (PED) induced by porcine virus (PEDV) is an intestinal infectious disease in pigs that causes serious economic losses to the pig industry. To develop effective oral vaccine against PEDV infection, we used a swine-origin Lactobacillus johnsonii (L. johnsonii) as antigen delivery carrier. A recombinant strain pPG-T7g10-COE/L. johnsonii-COE) expressing COE protein (a neutralizing epitope of viral spike protein) was generated. The immunomodulatory effect on dendritic...
Dendritic cells (DCs), as antigen-presenting cells, can initiate adaptive immune responses efficiently. Although the DC-targeting strategy has attracted more attention, relevant studies on chicken are rare. Here, specific bone marrow DC-binding peptides were selected using a phage display peptide library and confirmed through ELISA, flow cytometry, fluorescence microscopy, laser confocal microscopy. The candidate SPHLHTSSPWER, named SP, was fused to infectious bursal disease virus (IBDV)...
The number of co-infections with multiple porcine diarrhea viruses has increased in recent years. Inducing mucosal immunity through oral immunization is an effective approach for controlling these pathogens. To generate a multi-pathogen vaccine against viral co-infection, we employed the Lactobacillus vector platform, which was previously used to potent candidate vaccines various diseases. Two strategies were test protective efficiency recombinant viruses. First, mixture separately...
Porcine epidemic diarrhea (PED), characterized by diarrhea, vomiting, and dehydration, is an acute enteric infectious disease of pigs. The caused porcine virus (PEDV), which infects the intestinal mucosal surface. Therefore, immunization through oral route effective method immunization. Lactic acid bacteria, are resistant bile-salt improve immunity, ideal carriers for vaccines. S1 glycoprotein PEDV mediates binding with cell receptors induces neutralizing antibodies against virus. we...
Porcine reproductive and respiratory syndrome virus nonstructural protein 1 (nsp1) could be auto-cleaved into nsp1α nsp1β, both of which had the papain-like cysteine protease activities. Previous studies have shown that porcine nsp1 was an interferon (IFN) antagonist. However, mechanism by inhibited IFN-β production unclear. Here, we used site-directed mutagenesis inactivated activities to explore whether were required for disrupt production. The results showed mutations activity made lose...
Bacterial ghosts (BGs) are empty bacterial cell envelopes generated by releasing the cellular contents. In this study, a phage infecting Lactobacillus casei ATCC 393 (L. 393) was isolated and designated Lcb. We aimed at using L. as an antigen delivery system to express phage-derived holin for development of BGs.A gene fragment encoding Lcb (hocb) amplified polymerase chain reaction (PCR). used construct recombinant strain pPG-2-hocb/L. 393. Then recombinants were induced hocb. The...
Lactobacillus casei (L. casei) is well known for its probiotic property in human and animals. Lactoferricin (Lfcin) polypeptide can effectively modulate host immune responses have antimicrobial activity vivo vitro. In order to develop a food-grade L. system constitutively expressing bovine Lfcin, this study constructed thymidine auxotrophy (ΔthyA) recombinant casei.Based on the thymidylate synthase gene (thyA) insert site, LFEC(Lfcin expression cassette)was inserted into genome through...