A5008858680- Adenosine and Purinergic Signaling
- Pulmonary Hypertension Research and Treatments
- Gastroesophageal reflux and treatments
- IL-33, ST2, and ILC Pathways
- Polyamine Metabolism and Applications
- RNA modifications and cancer
- Adolescent and Pediatric Healthcare
- Immune Cell Function and Interaction
- Eosinophilic Esophagitis
- melanin and skin pigmentation
- Cancer-related gene regulation
- Extracellular vesicles in disease
- Enzyme-mediated dye degradation
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Biochemical and Molecular Research
- Nitrogen and Sulfur Effects on Brassica
- Plant tissue culture and regeneration
- Helicobacter pylori-related gastroenterology studies
- Cell Adhesion Molecules Research
- Water Quality and Pollution Assessment
- Protease and Inhibitor Mechanisms
- S100 Proteins and Annexins
- Antimicrobial Peptides and Activities
Institut Universitaire de Cardiologie et de Pneumologie de Québec
2022-2024
Université Laval
2013-2024
Montreal Heart Institute
2023
Centre hospitalier universitaire de Québec
2022
Centre hospitalier de l'Université Laval
2017
Extracellular vesicles (EVs) are involved in cell-to-cell communication and modulation of numerous physiological pathological processes. EVs found large quantities milk contain several inflammation- immunity-modulating proteins microRNAs, through which they exert beneficial effects inflammatory disease models. Here, we investigated the two EV subsets, concentrated from commercial cow's milk, on a murine model colitis induced with dextran sodium sulfate (DSS). P35K EVs, isolated by...
The ubiquitous polyamine spermidine is essential for cell survival and proliferation. One important function of to serve as a substrate hypusination, posttranslational modification process that occurs exclusively on eukaryotic translation factor 5A (eIF5A) ensures efficient various gene products. Pulmonary arterial hypertension (PAH) life-threatening disease characterized by progressive obliteration the small pulmonary arteries (PAs) caused excessive proliferation PA smooth muscle cells...
Objective Nucleotides are danger signals that activate inflammatory responses via binding P2 receptors. The nucleoside triphosphate diphosphohydrolase-8 (NTPDase8) is an ectonucleotidase hydrolyses receptor ligands. We investigated the role of NTPDase8 in intestinal inflammation. Design generated NTPDase8-deficient ( Entpd8 –/– ) mice to define dextran sodium sulfate (DSS) colitis model. To assess inflammation, colons were collected and analysed by histopathology, reverse...
Background and Purpose Ectonucleotidases control extracellular nucleotide levels consequently, their (patho)physiological responses. Among these enzymes, nucleoside triphosphate diphosphohydrolase‐1 ( NTPD ase1), −2, −3 −8 are the major ectonucleotidases responsible for hydrolysis at cell surface under physiological conditions, NTPDa se1 is predominantly located of vascular endothelial cells leukocytes. Efficacious inhibitors ase1 required to modulate responses induced by nucleotides in a...
Purinergic signaling plays a major role in T cell activation leading to IL-2 production and proliferation. However, it is unclear whether purinergic contributes the differentiation of effector cells. In this study, we found that receptor P2X4 was associated with human Th17 cells but not Th1 Inhibition specific antagonist 5-BDBD small interfering RNA inhibited development IL-17 by stimulated via CD3/CD28 pathway. Our results showed required for expression retinoic acid-related orphan C, which...
The ectonucleotidase nucleoside triphosphate diphosphohydrolase-8 (NTPDase8) is the last member of E-NTPDase family to be discovered and characterized. It a transmembrane protein which regulates concentration agonists P1 P2 receptors at cell surface. functions enzyme are still not known partly due lack specific tools such as antibodies. In this work, guinea pig polyclonal antibodies against mouse NTPDase8 monoclonal human have been generated For production several techniques tried. Several...
In this study we investigated the role of extracellular nucleotides in chemokine (KC, MIP-2, MCP-1 & CXCL10) expression and secretion by murine primary intestinal epithelial cells (IEC) with a focus on P2Y6 receptors. QRT-PCR experiments showed that was dominant nucleotide receptor expressed mouse IEC. addition, ligand UDP induced CXCL10. For other studies, took advantage mice deficient (P2ry6-/-). Similar levels P2Y1, P2Y2, P2X2, P2X4 A2A were detected P2ry6-/- WT Agonists TLR3 (poly(I:C)),...
Pulmonary arterial hypertension (PAH) is characterized by the narrowing of pulmonary arteries (PAs), causing right ventricular (RV) failure and premature death. artery smooth muscle cells (PASMCs) from PAH patients exhibit increased proliferation resistance to apoptosis in response extracellular matrix remodeling. Integrins are known be involved all these processes. Our hypothesis that integrin signaling promotes PASMCs resistance, leading PA remodeling, RV maladaptive hypertrophy fibrosis,...
An indiscriminate use of antibiotics in humans and animals has led to the widespread selection antibiotic-resistance, thus constricting antibiotics. A possible solution counter this problem could be develop alternatives that can boost host immunity, reducing quantity frequency antibiotic use. In work, for first time, citric acid laccase were used as extracellular inducers melanin production yeast cells human cell lines. It is proposed formulation together further promote...
MicroRNA (miR)-155-5p increases in innate and adaptive immune cells response to IL-13 is associated with the severity of asthma. However, little known about its role airway structural cells. Bronchial epithelial (BECs) isolated from healthy donors patients severe asthma were stimulated IL-13. miR-155-5p expression release measured by real-time (RT)-PCR BECs their derived exosomes. Modulation was performed using transfection inhibitor mimic. receptor α1 (IL-13Rα1), IL-13Rα2, MUC5AC, IL-8,...
Pulmonary arterial hypertension (PAH) is defined by increased pulmonary artery (PA) pressure and vascular remodeling, leading to progressive hypertrophy (compensation) failure (decompensation) of the right ventricle (RV). The histone acetyltransferases P300/CBP have been identified as central players in various cellular processes (proliferation/apoptosis hypertrophy/fibrosis). Given their role controlling gene transcription programs, we hypothesized that contributes maladaptive remodeling...
Pulmonary Arterial Hypertension (PAH) is characterized by progressive pulmonary arteries (PAs) obstruction leading to heart failure and death. PA smooth muscle cells (PASMCs) from PAH patients display a "cancer-like" phenotype contributing remodeling. Eukaryotic translation initiation factor 5A (eIF5A) provides cancer with competitive advantage increasing of mRNAs oncogenic proprieties. eIF5A the only protein containing unique spermidine-derived amino acid hypusine required for its function....
We have generated polyclonal and monoclonal antibodies by genetic immunization over the last two decades. In this paper, we present our most successful methodology acquired these years animals in which obtained highest rates of success. The technique presented is convenient, easy, affordable, generates against mammalian proteins their native form. This protocol requires neither expensive equipment, such as a gene gun, nor sophisticated techniques conjugation gold microspheres,...
<b>Introduction:</b> Asthma is a chronic disease characterized by prevailing type 2 inflammation and associated with airway remodeling. Exosomes emerged as regulators of cell-cell paracrine communication that transport DNA, lipids miRNA. Both exosomes miRNAs were recently shown to participate in asthma physiopathology. In asthmatics, have been promote inflammation, however, their role remodeling, modulation cargo under cytokine stimulation remain unclear asthma. <b>Objectives:</b> To...
Pulmonary arterial Hypertension (PAH) is characterized by vasoconstriction and remodeling of pulmonary arteries (PAs), causing right ventricular (RV) failure, premature death. Extensive proliferation PA smooth muscle cells (PASMCs) perhaps the most prominent feature PAH accounting for histopathological changes seen in this disease. With recognition, direct targeting vascular anti-proliferative approaches provides a promising strategy that holds potential to reverse process. To discover novel...
Pulmonary arterial hypertension (PAH) is a progressive occlusive vascular disease (OVD) in which pulmonary artery (PA) smooth muscle cells (PASMC) are characterized by excessive proliferation and resistance to apoptosis, like cancer cells. These features shared with neointimal hyperplasia coronary (CAD). ATP citrate lyase (ACLY), has recently emerged as key player sustain cellular survival favoring Warburg effect, chromatin acetylation, lipid synthesis. However, its role OVD remains unknown....
Introduction: Pulmonary Arterial Hypertension (PAH) is characterized by progressive distal pulmonary arteries (PAs) obstruction leading to heart failure and death. PA smooth muscle cells (PASMCs) from PAH patients display a “cancer-like” phenotype that contributes remodeling. Appreciation of the pivotal role translational control in hyperproliferating diseases steadily increasing. In this regard, eukaryotic translation initiation factor 5A (eIF5A) was shown provide cancer with competitive...
Introduction: Pulmonary arterial hypertension (PAH) is a lethal vasculopathy characterized by sustained vasoconstriction and remodeling of pulmonary arteries. Excessive proliferation reduced apoptosis artery smooth muscle cells (PASMCs) contribute to remodeling, with limited efficacy current pharmacotherapies. Increased DNA methylation (DNAm) has been implicated in pro-proliferative phenotypes disease progression cancer cardiovascular diseases. Hypothesis: We hypothesized that increased DNAm...