A5002661347- Neuroinflammation and Neurodegeneration Mechanisms
- Adenosine and Purinergic Signaling
- Immune Response and Inflammation
- Bone Tissue Engineering Materials
- Receptor Mechanisms and Signaling
- Mesenchymal stem cell research
- Cytokine Signaling Pathways and Interactions
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mast cells and histamine
- MicroRNA in disease regulation
- Gastroesophageal reflux and treatments
- Cancer-related molecular mechanisms research
- S100 Proteins and Annexins
- Immune Cell Function and Interaction
- Bone Metabolism and Diseases
- Heat shock proteins research
- Organ Transplantation Techniques and Outcomes
- Adolescent and Pediatric Healthcare
- Orthopaedic implants and arthroplasty
- interferon and immune responses
- Bone fractures and treatments
Université Laval
2009-2021
Centre hospitalier universitaire de Québec
2014-2015
Centre hospitalier de l'Université Laval
2014
Wilfrid Laurier University
2013
The University of Texas MD Anderson Cancer Center
2013
Institut Pasteur
2013
Inserm
2006-2008
Biomatériaux et Bioingénierie
2008
Muséum national d'Histoire naturelle
1976
Laboratoire d’Ethologie Expérimentale et Comparée
1976
Abstract Background Granulocytes generally exert protective roles in the central nervous system (CNS), but recent studies suggest that they can be detrimental experimental autoimmune encephalomyelitis (EAE), most common model of multiple sclerosis. While cytokines and adhesion molecules involved granulocyte to brain vasculature have started elucidated, required chemokines remain undetermined. Methods CXCR2 ligand expression was examined CNS mice suffering from EAE or exposed bacterial toxins...
Microbial agents can aggravate inflammatory diseases, such as multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). An example is pertussis toxin (PTX), a bacterial virulence factor commonly used an adjuvant to promote EAE, but whose mechanism of action unclear. We have reported that PTX triggers IL-6-mediated signaling cascade increases the number leukocytes patrol vasculature by crawling on luminal surface. In present study, we examined this...
Dysregulation of miRNAs has been observed in many neurodegenerative diseases, including multiple sclerosis. Morquette et al. show that overexpression miR-223-3p prevents accumulation axonal damage a rodent model sclerosis, part through regulation glutamate receptor signalling. Manipulation miRNA levels may have therapeutic potential.
Neutrophils contribute to demyelinating autoimmune diseases, yet their phenotype and functions have been elusive date. Here, we demonstrate that ICAM1 surface expression distinguishes extra- from intravascular neutrophils in the mouse CNS during experimental encephalomyelitis (EAE). Transcriptomic analysis of these 2 subpopulations indicated neutrophils, once extravasated, acquire macrophage-like properties, including potential for immunostimulation MHC class II-mediated antigen...
Objective Nucleotides are danger signals that activate inflammatory responses via binding P2 receptors. The nucleoside triphosphate diphosphohydrolase-8 (NTPDase8) is an ectonucleotidase hydrolyses receptor ligands. We investigated the role of NTPDase8 in intestinal inflammation. Design generated NTPDase8-deficient ( Entpd8 –/– ) mice to define dextran sodium sulfate (DSS) colitis model. To assess inflammation, colons were collected and analysed by histopathology, reverse...
Experimental autoimmune encephalomyelitis (EAE) is a model of inflammatory demyelinating diseases mediated by different types leukocytes. How these cells communicate with each other to orchestrate attacks not fully understood, especially in the case neutrophils, whose importance EAE newly established. The present study aimed determine expression pattern and role components IL-36 signaling pathway (IL-36α, IL-36β, IL-36γ, IL-36R) EAE.EAE was induced either active immunization myelin peptide,...
Abstract Oncostatin M (OSM) is a pleiotropic cytokine of the IL‐6 family and displays both pro‐inflammatory anti‐inflammatory activities. We studied impact OSM on gene activation profile human synovial cells, which play central role in progression inflammatory responses joints. In cells stimulated with lipopolysaccharide recombinant granulocyte‐macrophage colony‐stimulating factor, native secreted by granulocytes reduced expression secretion IL‐1β CXCL8, but increased that CCL2. This cell...
Proteinase-activated receptor-4 (PAR(4)) is a G-protein-coupled receptor activated by serine proteinases released during tissue repair and inflammation. We have previously shown that PAR(4) activation sensitises articular primary afferents leading to joint pain. This study examined whether mast cells contribute this PAR(4)-induced sensitisation consequent heightened pain behaviour. The expression of on synovial was confirmed with immunofluorescent staining rat knee sections....
Abstract Tissue engineering of autologous bone combined with osteoprogenitor cells is a suitable strategy for filling large defects. The aim this study was to evaluate the osteogenicity xenogenic graft cultured allogenic marrow stromal (BMSC) in mouse critical size craniotomy. Bovine trabecular grafts were made free or debris and delipidated. BMSC harvested from C57BL/6‐Tg(ACTbEGFP)1Osb/J mice (GFP + cells) 14 days on control osteogenic medium. Engineered implanted calvarial defect C57BL/6...
G protein–coupled receptor 84 (GPR84) is a 7-transmembrane protein expressed on myeloid cells that can bind to medium-chain free fatty acids in vitro. Here, we report the discovery of 2-bp frameshift deletion second exon Gpr84 gene several classical mouse inbred strains. This generates premature stop codon predicted result truncated lacking transmembrane domains 4-7. We sequenced 2 from 58 strains representing different groups family tree and found 14 are homozygous for deletion. Some these...
Adenosine, prostaglandin E2, or increased intracellular cyclic AMP concentration each elicit potent anti-inflammatory events in human neutrophils by inhibiting functions such as phagocytosis, superoxide production, adhesion and cytokine release. However, the endogenous molecular pathways mediating these actions are poorly understood. In present study, we examined their impact on gene expression profile of stimulated neutrophils. Purified blood from healthy donors were with a cocktail...
Abstract Multiple sclerosis (MS) is an autoimmune disease characterized by demyelination and neurodegeneration in the brain, spinal cord optic nerve. Neuronal degeneration death underlie progressive forms of MS cognitive dysfunction. damage triggered numerous harmful factors brain that engage diverse signalling cascades neurons thus therapeutic approaches to protect will need focus on agents can target broad biological processes. To spectrum signaling events mediate we have focused...
Histamine is best known for its role in allergies, but it could also be involved autoimmune diseases such as multiple sclerosis. However, studies using experimental encephalomyelitis (EAE), the most widely used animal model sclerosis, have reported conflicting observations and suggest implication of a nonclassical source histamine. In this study, we demonstrate that neutrophils are main producers histamine spinal cord EAE mice. To assess by taking into account different cellular sources,...