An appropriate cell microenvironment is key to tissue engineering and regenerative medicine. Revealing the factors that influence a fundamental research topic in fields of biology, biomaterials, engineering, The consists not only its surrounding cells soluble factors, but also extracellular matrix (ECM) or nearby external biomaterials regeneration. This review focuses on six aspects biomaterial–related microenvironments: ① chemical composition materials, ② material dimensions architecture, ③...

Abstract Since the pioneering work of Messersmith’s group discovering that polydopamine (PDA) can serve to adhere many types materials, PDA coating has, as a biomimetic approach, been widely used enhance cell adhesion by surface modification bind biologically active substances bioinert substrate. Nevertheless, it is unclear whether or not itself beneficial for cells. Herein, we report decreases viability cells under normal culture and observation conditions. Such an inhibition effect was...

The ability of cells to sense and respond mechanical signals from their surrounding microenvironments is one the key issues in tissue engineering regeneration, yet a fundamental study with both cell observation stimulus challenging should be based upon an appropriate microdevice. Herein we designed fabricated two-layer microfluidic chip enable simultaneous live cyclic stretching elastic polymer, polydimethylsiloxane (PDMS), modified surface for enhanced adhesion. Human mesenchymal stem...

The enrichment of co-reactants is one the keys to improving sensitivity electrochemiluminescence (ECL) detection. This work developed a novel hydrophobic localized strategy utilizing inner cavity β-cyclodextrin (β-CD). Pt nanoparticles (Pt NPs) were grown in situ on coordination sites for metal ions β-CD prepare β-CD-Pt nanocomposite, which could not only enrich co-reactant 3-(dibutylamino) propylamine (TDBA) highly efficiently through its but also immobilize TDBA via Pt–N bond. Meanwhile,...

Abstract While the microfluidic chips for cell stretching and real-time observations have so far been composed of three layers, present work reports a two-layer one, which is, on surface, not available due to ‘inherent’ difficulty unstable focusing cells in microscopic observation under operation, etc. Herein, this was overcome large extent, case appropriate device parameters, were determined based upon finite element analysis orthogonal experimental design. The novel chip fabricated...

While most studies of mechanical stimulation cells are focused on two-dimensional (2D) and three-dimensional (3D) systems, it is rare to study the effects cyclic stretching under a quasi-3D microenvironment as linkage between 2D 3D. Herein, we report new method prepare an elastic membrane with topographic microstructures integrate into microfluidic chip. The fabrication difficulty lay not only in preparation but also alignment bonding patterned other layers. To resolve problem, designed...

We developed a signal-on electrochemiluminescence (ECL) aptasensor by using SI-ATRP to facilitate high-density immobilization of luminophores and manganese dioxide–graphene (MnO2–GO) composite indirect deactivate the excited state Ru(dcbpy)32+ for ultrasensitive detection carcinoembryonic antigen (CEA). In this approach, served as an efficient quencher deactivating Ru(dcbpy)32+. Surface initiated atom transfer radical polymerization (SI-ATRP) was applied functionalize multiwalled carbon...

An electrochemiluminescence (ECL) immunoassay protocol was developed based on mimic-intramolecular interaction for sensitive detection of prostate specific antigen (PSA). It constructed by integrating the ECL luminophore (tris(4,4′-dicarboxylicacid-2,2′-bipyridyl)-ruthenium(II)dichloride (Ru(dcbpy)32+)) and coreactant (histidine) into supersandwich DNA structure. This strategy more effective in amplifying signal shortening electronic transmission distance, improving luminous stability...

To improve the toughness of PLA, Poly(lactic acid) (PLA)/organically modified montmorillonite (OMMT) nanocomposites were prepared via a masterbatching method. Melt index test indicated that had better processability compared with pure PLA. When loading MMT was 3 wt%, showed maximum tensile strength (63.81MPa), and its elongation at break increased by 2.6 times The thermal properties crystallization behaviors PLA studied Differential Scanning Calorimeter (DSC). With OMMT loaded, crystallinity...

The microcellular foaming PP/GF composites was made under the condition of twice-open mold by using chemical injection method, according to glass fiber reinforced mechanism, effect rule GF on mechanics properties studied in foam PP composites. results showed that tensile and impact strength Micro-foam one-step method were higher than micro-foam two-step when content is 30%, can reach 55.72MPa 7.5kJ/m 2 respectively，one-step get toughness

In this work, we present methods for the fabrication of lateral porous silicon membranes with size and charge selectivity, their monolithic integration into planar microfluidics as opposed to classical perpendicular pores that are used in hybrid 3D fluidic systems. Our approach is based on use locally patterned electrodes guide pore formation horizontally within membrane combination doped substrates spatially localize channel depth. Membranes tunable porosity were successfully fabricated by...

In this work, we demonstrate the transducing ability of lateral porous silicon membranes (LPSi) using optical interferometry. To aim, carry out Fourier-transform infrared spectroscopy measurements a microscope equipped with an appropriate objective in order to overcome difficulty obtain interference signal from LPSi small dimensions. Reflectance spectra are recorded while filling membrane various solvents and their analysis provide estimations effective thicknesses resulting index refraction...

Polylactic acid as a biodegradable polymer shows relative high rigidity, but it is brittle and has poor heat resistance, which greatly limits its application. The goal of this experiment to prepare polylactic acid/organic modified montmorillonite nanocomposite by melting, enhance the material properties improving interfacial compatibility. First PLLA-g-MAH prepared through reactive extrusion under initiator dicumyl peroxide, then PLLA /PLLA-g-MAH/OMMT melt extrusion. Grafted rate was...

In this work, we present a novel fabrication process for the creation of lateral porous silicon membranes with tunable pore size and porosity their integration into planar microfluidics. Since formation by anodization is highly dependent on dopant type concentration, use ion implantation to manipulate local concentration in order control membrane properties. The dead-end filtration capability our integrated within microchannels demonstrated adequately retaining 300 nm diameter beads, while...

Long glass fiber (LGF)‐reinforced polypropylene (PP) composites were prepared using self‐designed impregnation device. Effects of time on mechanical properties, crystallization, dynamic and morphology PP/LGF investigated. The experiment results demonstrate that the excellent tensile strength, Notched Izod impact strength was 152.9 MPa, 31.2 KJ/m 2 , respectively, stiffness higher, when 7.03 s. interfacial adhesion between PP indicates possess outstanding properties. scarcely influenced...

The tensile model of foams is built using UG software and the deformation processes microcellular polypropylene with different cell sizes are simulated finite element method (FEM). effect size on mechanical property evaluated based microstructure foams. cells small uniform in a state plane stress, which improved effectively Whereas large nonuniform strain, leads to low property. simulation results coincide well experimental results.

Poly(L-lactic acid ) (PLLA) /oganic montmorillonite (OMMT) composites were prepared by means of melt-mixing using a twin-screw extruder. The mechanical properties and melt flow rate the investigated, their crystallization structure thermal characterized X-Ray Diffraction (XRD), Differential Scanning Calorimeter (DSC), thermogravimetric analysis (TG). It was shown that tensile strength, elongation at break bending strength PLLA/OMMT firstly increased then reduced along with increase content...