A5090947144- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Empathy and Medical Education
- Urinary Bladder and Prostate Research
- Anatomy and Medical Technology
- Innovations in Medical Education
- RNA regulation and disease
- Cerebrospinal fluid and hydrocephalus
- Anesthesia and Pain Management
- Fetal and Pediatric Neurological Disorders
- Multiple Sclerosis Research Studies
- interferon and immune responses
- Urological Disorders and Treatments
- Optical Imaging and Spectroscopy Techniques
- Telemedicine and Telehealth Implementation
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Organ Donation and Transplantation
- Case Reports on Hematomas
- Sexual function and dysfunction studies
- NF-κB Signaling Pathways
- Myofascial pain diagnosis and treatment
- Blood properties and coagulation
Montreal Neurological Institute and Hospital
2021-2025
McGill University
2021-2025
Western University
2017-2021
Microglia are tissue resident macrophages with a wide range of critically important functions in central nervous system development and homeostasis.In this study, we aimed to characterize the transcriptional landscape ex vivo human microglia across different developmental ages using cells derived from pre-natal, pediatric, adolescent, adult brain samples. We further confirmed our observations ELISA RNAscope.We showed that pre-natal have distinct regulatory signature relative their post-natal...
Abstract Ependymal cells form a specialized brain–cerebrospinal fluid (CSF) interface and regulate local CSF microcirculation. It is becoming increasingly recognized that ependymal assume reactive state in response to aging disease, including conditions involving hypoxia, hydrocephalus, neurodegeneration, neuroinflammation. Yet what transcriptional signatures govern these states whether this reactivity shares any similarities with classical descriptions of glial (i.e., astrocytes) remain...
Abstract It is now widely recognized that the cerebrospinal fluid (CSF)-adjacent brain surfaces – namely subpial cortical region and ependyma-adjacent periventricular are uniquely susceptible to a distinct, diffuse form of pathology in multiple sclerosis. So-called surface-in gradients predict future disease relapses independent classical white matter lesions thought occur as result cytotoxic factors CSF. Given underlying mechanisms driving appear be they represent novel treatment target....
Primary ciliary dyskinesia (PCD) is a genetic disorder causing motile dysfunction primarily affecting the respiratory and reproductive systems. However, impact of PCD on central nervous system remains poorly understood. Rodent models exhibit marked hydrocephalus leading to early animal mortality, however, most humans with do not develop for unknown reasons. We hypothesized that patients sub-clinical ventriculomegaly related ependymal dysfunction. demonstrated highly specific expression...
Highlights•RGCs demonstrate similar gene expression changes during EAE and aging•RGCs exhibit DNA damage, chromatin mark changes, nuclear lamina dystrophy in EAE•Patients with MS damage senescence-associated neurons•AAV2-OSK limits RGC death preserves visual acuity EAESummaryIn multiple sclerosis (MS), inflammation of the central nervous system results demyelination, neuroaxonal injury, cell death. However, molecular signals responsible for injury neurons are not fully characterized. Here,...
Mature oligodendrocytes form myelin sheaths that are crucial for the insulation of axons and efficient signal transmission in central nervous system. Recent evidence has challenged classical view functionally static mature oligodendrocyte revealed a gamut dynamic functions such as ability to modulate neuronal circuitry provide metabolic support axons. Despite recognition potential heterogeneity function, comprehensive summary diversity is lacking. We delve into early 20 th -century studies...
Abstract Primary ciliary dyskinesia (PCD) is a genetic disorder causing motile dysfunction primarily affecting the respiratory and reproductive systems. However, impact of PCD on central nervous system, through cilia in multiciliated ependymal cells, remains poorly understood. We hypothesized that patients with exhibit sub-clinical ventriculomegaly due to dysfunction, which may influence neuropsychiatric diagnoses. demonstrated highly specific expression levels known PCD-related genes human...
In multiple sclerosis (MS), the invasion of central nervous system by peripheral immune cells is followed activation resident microglia and astrocytes. This cascade events results in demyelination, which triggers neuronal damage death. The molecular signals neurons responsible for this are not yet fully characterized. MS, retinal ganglion cell (RGCs) (CNS) undergo axonal injury phenomenon mirrored experimental autoimmune encephalomyelitis (EAE) mouse model MS. To understand landscape, we...
Injury to the nerves of aortic- and superior hypogastric plexuses during retroperitoneal surgery often results in significant post-operative complications, including retrograde ejaculation and/or loss seminal emission males. Although previous characterizations these have done well provide a basis for understanding typical anatomy, additional research into common variations could further optimize nerve-sparing techniques surgery. To achieve this, present study aimed document prevalence...
Abstract Objective Multiple sclerosis is an inflammatory demyelinating disorder associated with blood-brain-barrier breakdown, where myelin repair reduced and ultimately fails. Our aim was to investigate the effect of a systemically circulating molecule fibrinogen, which abnormally present in central nervous system as result blood-brain barrier breakdown on human oligodendrocyte lineage cells. Methods In situ immunofluorescence performed using anti-fibrinogen anti-SOX10 antibodies. Mature...
Ependymal cells make up the epithelial monolayer that lines brain ventricles and spinal cord central canal are filled with cerebrospinal fluid. The ependyma has several functions, including regulating solute exchange between fluid parenchyma, controlling microcirculation of via coordinated ciliary beating, acting as a partial barrier. Dysregulation these functions can lead to waste clearance impairment, accumulation, hydrocephalus, more. A role for ependymal in variety neurological disorders...
Ejaculatory dysfunction is a significant cause of infertility in men that have incurred spinal cord injury or iatrogenic lesions to the sympathetic nerves retroperitoneum. For such patients, electroejaculation – whereby voltage applied transrectally under general anesthesia highly-effective procedure obtain ejaculate. At present, however, there remains uncertainty as physiological mechanism by which prompts seminal emission males with neurogenic anejaculation. Thus, present study, we aimed...
Anatomy education continues to evolve in health professional programs as curricula shift competency-based models and contact hours decrease. These changes may significantly alter the learning environment for students. Importantly, have been shown impact student strategies well-being. It follows, then, that an investigation of students' perceptions is key understand modern anatomy curriculum alterations. The current pilot study evaluated modifying examination format on physical therapy...
Wilder Penfield is known for his contributions to the structure-function relationship of brain and surgical treatment focal epilepsy. Less well are study glial cells investigation their role in human neuropathology. learned gold silver methods staining neurons, cells, projections from Charles Sherrington Pío del Río-Hortega. He colleague William Cone established a laboratory neuropathology using metallic stains, initially at Presbyterian Hospital New York City 1925, then Montreal...
<title>Abstract</title> Autopsy-derived brain tissue analysis is vital for exploring the complex landscape of neurobiology in health and disease but processing conditions during <italic>post-mortem</italic> handling can lead to significant technical artifacts affecting data interpretation. Here, we define transcriptomic signatures from healthy adult human that was snap frozen under 1 hour (Mean: 31min, Min: 10min, Max 55min) extraction (hereafter referred as ~ 0 hours) compared it autopsy...
Abstract The human meninges are a dynamic tri-layered brain border that plays key role in development, CSF homeostasis, immune regulation, and higher-level function. have also been implicated central nervous system (CNS) pathologies such as infection, autoimmunity, trauma. To understand how the meningeal microenvironment is altered under pathological conditions it necessary to complete understanding of its normotypic cellular architecture date, there no atlas adult meninges. By surgically...
For decades, ultrastructural evaluation of epithelial cells in diverse organ systems has demonstrated the existence two subtypes identified by stark differences cytoplasmic electron density — so-called light and dark cells. Choroid plexus (CP) are key regulators CSF homeostasis one many specialized linings that exhibit this bimodal phenotype. Despite longstanding acknowledgement, it been difficult to assess potential significance adult human CP due a lack characterization beyond microscopy...