A5079717296- Neuroinflammation and Neurodegeneration Mechanisms
- Multiple Sclerosis Research Studies
- Barrier Structure and Function Studies
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Axon Guidance and Neuronal Signaling
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Apelin-related biomedical research
- Cardiovascular Health and Disease Prevention
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Blood properties and coagulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Acute Ischemic Stroke Management
- Delphi Technique in Research
- S100 Proteins and Annexins
- Cytokine Signaling Pathways and Interactions
- Psoriasis: Treatment and Pathogenesis
- Hedgehog Signaling Pathway Studies
- Immune cells in cancer
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Inflammasome and immune disorders
- Atherosclerosis and Cardiovascular Diseases
- Immune Cell Function and Interaction
- Pleural and Pulmonary Diseases
Centre Hospitalier de l’Université de Montréal
2014-2024
Université de Montréal
2014-2024
McGill University
2022
Hasselt University
2020
Blood–brain barrier function is driven by the influence of astrocyte-secreted factors. During neuroinflammatory responses blood–brain compromised resulting in central nervous system damage and exacerbated pathology. Here, we identified endothelial netrin 1 induction as a vascular response to astrocyte-derived sonic hedgehog that promotes autocrine properties during homeostasis increases with inflammation. Netrin supports integrity upregulating junctional protein expression, while knockout...
Significance Multiple sclerosis (MS) is an inflammatory disorder characterized by multifocal lesions in the central nervous system. These are caused infiltrating leukocytes that take advantage and/or actively participate disruption of blood–brain barrier (BBB). In this study, specific role adhesion molecule ALCAM (activated leukocyte cell molecule) present on BBB endothelial cells was assessed. We demonstrated knockout mice develop a more severe experimental autoimmune encephalomyelitis,...
Activated leukocyte cell adhesion molecule mediates B lymphocyte migration across central nervous system barriers.
DICAM contributes to the pathogenesis of autoimmune neuroinflammation by promoting migration T H 17 lymphocytes across blood-brain barrier.
Multiple sclerosis (MS) is a neuroinflammatory and neurodegenerative disease characterized by infiltration of immune cells in multifocal areas the CNS. The specific molecular processes allowing autoreactive to enter CNS compartment through blood-brain barrier remain elusive.Using endothelial cell (EC) enrichment single-cell RNA sequencing, we implicated experimental autoimmune encephalomyelitis, an animal model MS. Validations on human MS brain sections most differentially expressed genes...
The trafficking of autoreactive leucocytes across the blood-brain barrier endothelium is a hallmark multiple sclerosis pathogenesis. Although represents one main CNS borders to interact with infiltrating leucocytes, its exact contribution neuroinflammation remains understudied. Here, we show that Mcam identifies inflammatory brain endothelial cells pro-migratory transcriptomic signature during experimental autoimmune encephalomyelitis. In addition, MCAM was preferentially upregulated on in...
To investigate the involvement of interleukin (IL)-26 in neuroinflammatory processes multiple sclerosis (MS), particular blood-brain barrier (BBB) integrity.Expression IL-26 was measured serum, CSF, vitro differentiated T helper (TH) cell subsets, and postmortem brain tissue patients with MS controls by ELISA, quantitative PCR, immunohistochemistry. Primary human mouse BBB endothelial cells (ECs) were treated assessed for integrity. RNA sequencing performed on IL-26-treated ECs. Myelin...
The coordinated function of beta cells within the pancreatic islet is required for normal regulation insulin secretion and partly controlled by specialized "leader" highly connected "hub" beta-cell subpopulations. Whether these subpopulations are functionally stable in vivo remains unclear. Here, we establish an approach to monitor Ca 2+ dynamics individual over time, after engraftment into anterior eye chamber, where continuous blood perfusion near innervation pertain. Under normoglycemic...
In multiple sclerosis (MS), peripheral immune cells use various cell trafficking molecules to infiltrate the CNS where they cause damage.The objective of this study was investigate involvement coxsackie and adenovirus receptor-like membrane protein (CLMP) in migration into patients with MS.Expression CLMP measured primary cultures human brain endothelial (HBECs) meningeal (HMECs), postmortem samples, blood mononuclear (PBMCs) from MS controls by RNA sequencing, quantitative PCR,...
Abstract Objective Multiple sclerosis is an inflammatory demyelinating disorder associated with blood-brain-barrier breakdown, where myelin repair reduced and ultimately fails. Our aim was to investigate the effect of a systemically circulating molecule fibrinogen, which abnormally present in central nervous system as result blood-brain barrier breakdown on human oligodendrocyte lineage cells. Methods In situ immunofluorescence performed using anti-fibrinogen anti-SOX10 antibodies. Mature...
TCR1640 mice, which have a T cell receptor (TCR) directed against MOG92–106, spontaneously develop experimental autoimmune encephalomyelitis. Female mice mostly relapsing-remitting (RR) course and higher incidence of disease, while males most frequently suffer from progressive reflecting the unresolved clinical sex discrepancies seen in multiple sclerosis. Herein, we performed adoptive transfers male female immune cells into WT animals to investigate if disease is dependent on donor or...
<title>Abstract</title> Oxidized phosphatidylcholines (OxPC) are neurotoxic byproducts of oxidative stress elevated in the central nervous system (CNS) during progressive multiple sclerosis (P-MS). How OxPC contribute to pathophysiology P-MS is unclear. Here, we report that deposition CNS mice induces a chronic compartmentalized lesion with pathological features similar active lesions found P-MS. Using this new model, while microglia protected from neurodegeneration, they were also replaced...
Multiple sclerosis (MS) is a neuroinflammatory and neurodegenerative disease characterized by infiltration of immune cells in multifocal areas the central nervous system (CNS). The specific molecular processes allowing autoreactive to enter CNS compartment through blood-brain barrier (BBB) remain elusive. Using endothelial (ECs) enrichment single-cell RNA sequencing (scRNA-Seq), we implicated experimental auto-immune encephalomyelitis, an animal model MS. We found upregulation genes...