A5064653137- RNA and protein synthesis mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Diet and metabolism studies
- DNA and Nucleic Acid Chemistry
- Hormonal Regulation and Hypertension
- Diet, Metabolism, and Disease
- Genomics and Chromatin Dynamics
- Aquaculture Nutrition and Growth
- Animal Genetics and Reproduction
- Reproductive biology and impacts on aquatic species
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Estrogen and related hormone effects
- Enzyme Structure and Function
- Phytochemicals and Antioxidant Activities
- Stress Responses and Cortisol
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Protein Kinase Regulation and GTPase Signaling
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Steroid Chemistry and Biochemistry
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Diabetes and associated disorders
- Nuclear Physics and Applications
- Thermoregulation and physiological responses
Università di Camerino
2002-2022
The presence of pituitary adenylate cyclase-activating peptide (PACAP) 38-immuno-like material (PACAP 38-IL) in the brain and ovary crested newt, Triturus carnifex, its action on ovarian steroidogenesis prostaglandin synthesis were evaluated. HPLC extract peaks that eluted like PACAP 38 considered 38-like material. concentrations 38-IL extracts measured by RIA. T. carnifex was incubated with 38, 38-IL, inhibitors cyclooxygenase (COX), cyclase (AC) phospholipase C (PLC) for 30 60 min....
The pentapeptide pyroGlu‐Ala‐Glu‐Ser‐Asn has been synthetized and phosphorylated in vitro at level of serine by protein kinase NII isolated from calf thymus chromatin. It is noteworthy that the shows a remarkable affinity for this peptide. [ 32 P]peptide able to bind several DNAs presence Mg 2+ (λ phage, thymus, pBR540 plasmid). This binding appears not specific with regard type DNA its base sequence. These data support hypothesis acidic domains nuclear nonhistone proteins could directly cations
The World Health Organization has identified antimicrobial resistance as a substantial threat to human health. Because of the emergence pathogenic bacteria resistant multiple antibiotics worldwide, there is need identify mode action and unravel basic mechanisms responsible for drug resistance. Antibiotic producers’ microorganisms can protect themselves from toxic effect using different strategies; one most common involves modification antibiotic’s target site. In this work, we report...
Diets rich in foods containing antioxidants, such as fruits and vegetables, could help to prevent cardiovascular diseases, inflammatory neurodegenerative disorders, cancer slow the aging process. The aim of this work was look at seed sprouts a member Brassica genus, Savoy cabbage (Brassica oleracea L. var. sabauda) determine antioxidant activity aqueous hydroalcoholic extracts made from powdered form its sprouts. vitro tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH)...
Our aim was to study the role of angiotensin-converting enzyme (ACE) and angiotensin II (ANG II) on ovarian steroidogenesis prostaglandin production amphibian. Hormonal effects ACE, ACE inhibitors, synthetic bullfrog I I), [Val5]ANG were compared frog ovaries postreproductive prereproductive periods. Very high activity found in ovary water (Rana esculenta) with other tissues, this inhibited by typical captopril lisinopril. Frog tissue periods incubated vitro presence (2.5 mU/ml), (0.1 mM),...
The occurrence of angiotensin converting enzyme (EC 3.4.15.1; ACE) was demonstrated for the first time in serum newt ( Triturus carnifex ) and frog Rana esculenta ). enzymatic activity evidenced following hydrolysis N‐[3‐(2‐furyl) acryloyl]L‐phenylalanyl glycyl glycine (FAPGG), a synthetic substrate ACE. liberated acryloyl]L‐phenylalanine (FAP) from FAPGG. properties amphibian enzymes were compared with those swine. FAPGG hydrolysing activities inhibited by typical ACE inhibitors, captopril...
Abstract Vitellogenin (Vtg) has proven to be a sensitive and simple biomarker in determining sex, sexual maturity, xenoestrogenic effects fish. Thus, our investigation been focused on identification, partial characterization, quantification of grey mullet ( Mugil cephalus ) Vtg through the use variety biochemical immunological analytical techniques. Mullet is considered both promising aquaculture candidate an important species for improving sediment quality polyculture systems. In first part...
The aim of the present research was to study role angiotensin-converting enzyme (ACE) and ANG II in amphibian (Rana esculenta) testicular steroidogenesis prostaglandin production. Hormonal effects ACE, ACE inhibitors, synthetic bullfrog I, [Val(5)]ANG were determined frog testis prereproductive period. Production 17beta-estradiol, progesterone, androgens, PGE(2) PGF(2alpha) by incubating testes with (2.5 mU/ml), captopril (0.1 mM), lisinopril (1 microM), I microM). analysis data showed an...
This research was undertaken to determine the glycoconjugates secreted by glandular epithelium of seminal vesicles in entire and castrated horses using lectin histochemistry combination with sialidase digestion deglycosylation pre-treatments. The following lectins were used: Con-A, UEA-I, LTA, WGA, GSA-IB4, SBA, PNA, ECA DBA. In stallion, cells expressed sugar residues: α-Fuc, internal GlcNAc, terminal β- α-GalNAc, β-D-Gal-(1–4)-GlcNAc α-Gal included O-linked oligosaccharides;...
This study was conducted to determine the presence of angiotensin converting enzyme in commercial sera used cell culture medium. The aim research bring proteinases (angiotensin enzyme) users' knowledge and give some data for solving problems about development peptides as useful drugs. enzymes, purified from foetal bovine, adult equine, human sera, showed molecular weights 170 kDa. Captopril lisinopril inhibited activities at nanomolar concentrations. enzymes were able hydrolyze, with...
Abstract The Authors describe the purification of swine serum ACE to molecular homogeneity with high recovery activity (40 %) in a few stepsl. procedure consists affinity chromatography, using commercial activated resin (epoxy‐activated sepharose 6B) and two steps anion exchange chromatography (Resource Q) performed at different pH (pH 9.0 6.0). Furthermore, specific sensitive method for accurate quantitation biological fluids was developed, based on hydrolysis synthetic FAPGG...