Adipocytes arise from distinct progenitor populations during developmental and adult stages but little is known about how progenitors differ progenitors. Here, we investigate the role of platelet-derived growth factor receptor alpha (PDGFRα) in divergent regulation two different adipose cells (APCs). Using vivo lineage tracking deletion mouse models, found that PDGFRα+ are adipogenic differentiated into mature adipocytes, Pdgfra disrupted white tissue (WAT) formation. Interestingly, do not...

Dietary fatty acids play important roles in the regulation of fat accumulation or metabolic phenotype adipocytes, either as brown beige fat. However, a systematic comparison effects diets with different composition 18-C on browning/beiging has not been done. In this study, we compared dietary fats, rich specific 18-carbon acids, thermogenesis and lipid metabolism. Male C57BL/6 mice were fed control diet containing 5.6% kcal from lard 4.4% soybean oil (CON) high-fat (HFD) 25% 20% shea butter...

Abstract The study was conducted to determine the effects of high levels phytase on growth performance, nutrient digestibility, phytate breakdown, and expression mucosal tight junction transporter genes in weanling pigs. A total 128 barrows were penned groups four used a randomized completely block design assigned treatments for 28-d study. two-phase feeding implemented (phase 1: day 1 14; phase 2: 15 28). diets differed dietary calcium (Ca) phosphorus (P) (positive control [PC]: 8.1 7.1...

The health benefits of dietary fiber are widely recognized, but its impact on muscle remains unclear. Therefore, this study aimed to elucidate the relationship between intake and strength through a cross-sectional analysis data from Korea National Health Examination Survey (KNHANES). Data single 24-h recall handgrip tests 10,883 younger adults aged 19 64 years 3,961 older ≥ 65 were analyzed. Low was defined as < 28 kg for men 18 women. Multivariable linear logistic regression analyses...

Perilla frutescens var. acuta (Lamiaceae) is widely used not only as an oil or a spice, but also traditional medicine to treat colds, coughs, fever, and indigestion. As ongoing effort, luteolin-7-O-diglucuronide (1), apigenin-7-O-diglucuronide (2), rosmarinic acid (3) isolated from P. were investigated for their anti-adipogenic thermogenic activities in 3T3-L1 cells. Compound 1 exhibited strong inhibition against adipocyte differentiation by suppressing the expression of Pparg Cebpa over...

In this study, we investigated effects of pine nut oil (PNO) on high-fat-diet (HFD)-induced obesity and metabolic dysfunction in skeletal muscle brown adipose tissue (BAT). Male C57BL/6 mice were fed a HFD with 15% energy from lard 30% either soybean (SBO-HFD) or PNO (PNO-HFD) for 12 weeks. The PNO-HFD resulted less weight gain intramuscular lipid accumulation than the SBO-HFD was accompanied by upregulation transcripts proteins related to oxidative metabolism phosphorylation AMP-activated...

Rickettsia monacensis, a spotted fever group rickettsia, was isolated from Ixodes nipponensis ticks collected live-captured small mammals in South Jeolla province, Korea 2006. Homogenates of tick tissues were inoculated into L929 and Vero cell monolayers using shell vial assays. After several passages, Giemsa staining revealed rickettsia-like organisms the cells, but not cells. Sequencing analysis that ompA-small part (25-614 bp region), ompA-large (2849-4455 nearly full-length ompB (58-4889...

Korean pine nut oil (PNO) has been reported to have favorable effects on lipid metabolism and appetite control.We investigated whether PNO consumption could influence weight gain, the PNO-induced effect would result in an improvement of immune function high-fat diet (HFD)-induced obese mice.C57BL/6 mice were fed control diets with 10% energy fat from either or soybean (SBO), HFDs 45% SBO 35% lard, 20% 25% 30% 15% lard for 12 weeks.The proliferative responses splenocytes upon stimulation...

Korean pine nut oil (PNO) has been reported to influence weight gain and lipid metabolism. We examined whether PNO replacement in a high-fat diet (HFD) can ameliorate HFD-induced hepatic steatosis. Five-week-old male C57BL mice were fed control diets containing 10% of the energy from fat or soybean (SBO) (PC, SC) HFDs with 45% fat, SBO 35% lard (PHFD, SHFD), for 12 weeks. Body amount white adipose tissue lower PHFD (10% 18% lower, respectively) compared SHFD. Hepatic triacylglycerol (TG)...

Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim this study examine the effects PNO on both intestinal lipid metabolism control or HFD.Five-week-old C57BL/6 were diets containing 10% energy fat from either Soybean Oil (SBO) PNO, HFD 15% lard 30% SBO for 12 weeks. Expression genes related fatty acid (FA) uptake channeling (Cd36, Fatp4, Acsl5, Acbp), chylomicron synthesis (Mtp, ApoB48, ApoA4), (Lrp1,...

Abstract Long‐chain fatty acids (LCFA) are known to activate brown and beige adipocytes. However, very little is about the effects of number position double bonds in LCFA with same length on fat‐specific gene expression. To determine specificity regulation these genes different adipocyte models, fully differentiated 10T1/2, 3T3‐L1, murine, or porcine primary adipocytes (obtained from subcutaneous fat pad C57BL/6 mice Landrace × Yorkshire Duroc crossbred piglets) were treated 50 μM following...

Neurophysin (Np) is generally found in close association with vasopressin and oxytocin the hypothalamo-neurohypophyseal complex. Dog neurophysin I II have been isolated from fresh frozen posterior pituitaries. The proteins were characterized on basis of disc electrophoresis, immunological properties, amino acid composition partial sequence determination. terminal dog Np Ala-Ala-Leu-Asp-Leu-Asp-Val-Arg-Gln-Cys-Leu-Pro-Cys-Gly-Pro-Gly-Gly-Gln-Gly-while that Np-II Ala-Met-Ser-Asp-Leu-Glu-Leu....

This study was done to characterize distribution of Rickettsia spp. in ticks the northwestern and southwestern provinces Republic Korea. A total 2,814 were collected between May September 2009. After pooling, 284 tick DNA samples screened for a gene Rickettsia-specific 17-kDa protein using nested PCR (nPCR), produced 88 nPCR positive samples. Of these positives, 75% contained 190-kDa outer membrane (ompA), 50% 120-kDa (ompB), 64.7% D (sca4). The products ompA, ompB, sca4 genes revealed close...

Perilla frutense var. acuta (Lamiaceae) has been used to treat indigestion, asthma, and allergies in traditional medicine. In this study, luteolin 7-O-diglucuronide (1), apigenin (2), rosmarinic acid (3) were isolated from the leaves of P. frutescens acuta. Various approaches performed investigate anti-inflammatory activity compounds (1-3) their working mechanisms. silico docking simulation demonstrated that 1-3 would work as a PPAR-&amp;alpha;/&amp;delta;/&amp;gamma; agonist, vitro...

Abstract Fatty acids are involved in the regulation of inflammatory responses obesity. However, despite being largest dietary fatty‐acid class, effects 18‐carbon fatty with different degrees saturation on inflammatory, metabolic, and thermogenic markers have not been well studied. Therefore, objective this study was to test if diets profiles differentially regulate metabolic genes. Male C57BL/6 mice were fed one four diets: a control diet (CON) containing 5.6% kcal fat from lard 4.4% soybean...

A total of 9,281 larval chigger mites were collected from small mammals captured at Hwaseong-gun, Gyeonggi-do (Province) (2,754 30 mammals), Asan city, Chungcheongnam-do (3,358 48 and Jangseong-gun, Jeollanam-do (3,169 for 62 mammals) April-November 2009 in the Republic Korea (= Korea) identified to species. Leptotrombidium pallidum was predominant species Hwaseong (95.8%) (61.2%), while scutellare Jangseong (80.1%). Overall, mite indices decreased April (27.3) June (4.9), then increased...

The purpose of this study was to explore the laboratory biosafety status Public Health Centers (PHCs) in Korea during Oct.7∼26, 2012. We surveyed environment management, especially for recognition level workers organizations. questionnaires given out 98 who are working PHCs research knowledge biosafety, related laws and regulations. highest Research Institute & Environment (RIPHE) followed by quarantine station, health center assessed as last. It turned that educational program RIPHE...