A5052364266- Advanced biosensing and bioanalysis techniques
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Neural and Behavioral Psychology Studies
- Antibiotics Pharmacokinetics and Efficacy
- Monoclonal and Polyclonal Antibodies Research
- Neurotransmitter Receptor Influence on Behavior
- Drug Transport and Resistance Mechanisms
- Asthma and respiratory diseases
- Memory and Neural Mechanisms
- Biosensors and Analytical Detection
- Tuberculosis Research and Epidemiology
- CO2 Reduction Techniques and Catalysts
- Receptor Mechanisms and Signaling
- Anodic Oxide Films and Nanostructures
- Catalytic Cross-Coupling Reactions
- Bacterial Genetics and Biotechnology
- Carbon dioxide utilization in catalysis
- Acute Lymphoblastic Leukemia research
- Control Systems and Identification
- SARS-CoV-2 detection and testing
- Metabolomics and Mass Spectrometry Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Pregnancy and Medication Impact
University of California, Santa Barbara
2019-2025
University of California, Los Angeles
2017-2018
Amgen (United States)
2008
The electrochemical aptamer-based (E-AB) sensing platform appears to be a convenient (rapid, single-step, and calibration-free) modular approach measure concentrations of specific molecules (irrespective their chemical reactivity) directly in blood even situ the living body. Given these attributes, may thus provide significant opportunities render therapeutic drug monitoring (the clinical practice which dosing is adjusted response plasma measurements) as frequent measurement sugar has become...
Electrochemical aptamer-based sensors enable real-time molecular measurements in the living body. The spatial resolution of these and ability to perform targeted locations, however, is limited by length width device's working electrode. Historically, achieving good signal noise complex, noisy vivo environment has required electrode lengths 3–6 mm. To sensor miniaturization, here we have enhanced signaling current obtained for a given macroscopic dimensions increasing its surface area....
Current knowledge of the disposition kinetics endogenous metabolites is founded almost entirely on poorly time-resolved experiments in which samples are removed from body for later, benchtop analysis. Here, contrast, we describe real-time, seconds-resolved measurements plasma phenylalanine collected situ via electrochemical aptamer-based (EAB) sensors, a platform technology that independent reactivity its targets and thus generalizable to many. Specifically, using indwelling EAB have...
Recent years have seen the development of a number biosensor architectures that rely on target binding-induced changes in rate electron transfer from an electrode-bound receptor. Most often, interrogation these sensors has relied voltammetric methods, such as square-wave voltammetry, which limit their time resolution to few seconds. Here, we describe use impedance-based approach, termed electrochemical phase interrogation, means collecting high measurements with this class. Specifically,...
Abstract Electrochemical aptamer-based (EAB) sensors support the real-time, high frequency measurement of pharmaceuticals and metabolites in-situ in living body, rendering them a potentially powerful technology for both research clinical applications. Here we explore quantification using EAB sensors, examining impact media selection temperature on performance. Using freshly-collected, undiluted whole blood at body as our calibration conditions, demonstrate accuracy better than ± 10% test bed...
Dose-limiting toxicity and significant patient-to-patient pharmacokinetic variability often render it difficult to achieve the safe effective dosing of drugs. This is further compounded by slow, cumbersome nature analytical methods used monitor patient-specific pharmacokinetics, which inevitably rely on blood draws followed post-facto laboratory analysis. Motivated pressing need for improved "therapeutic drug monitoring", we are developing electrochemical aptamer-based (EAB) sensors, a...
Knowledge of drug concentrations in the brains behaving subjects remains constrained on a number dimensions, including poor temporal resolution and lack real-time data. Here, however, we demonstrate ability electrochemical aptamer-based sensors to support seconds-resolved, measurements freely moving rats. Specifically, using such sensors, achieve <4 μM limits detection 10-s measurement procaine rats, permitting determination pharmacokinetics concentration-behavior relations with high...
EAB sensors stored at −20 °C for least six months perform comparably to freshly fabricated sensors.
Electrochemical aptamer-based (EAB) sensors are capable of measuring the concentrations specific molecules in vivo, real time, and with a few-second time resolution. For their signal transduction mechanism, these utilize binding-induced conformational change target-recognizing, redox-reporter-modified aptamer to alter rate electron transfer between reporter supporting electrode. While variety voltammetric techniques have been used monitor this kinetics, they suffer from various drawbacks,...
Electrochemical aptamer-based (EAB) sensors represent the first molecular measurement technology that is both (1) independent of chemical reactivity target, and thus generalizable to many targets (2) able function in an accurate, drift-corrected manner situ living body. Signaling EAB generated when electrode-bound aptamer binds its target ligand, altering rate electron transfer from attached redox reporter producing easily detectable change peak current sensor interrogated using square wave...
Background and Purpose Pharmacokinetics have traditionally been assessed using concentration measurements with relatively low temporal resolution, such as from blood draws, leading to pharmacokinetic profiles estimated sparse data, often averaged across subjects. Recent advances in vivo sensors, however, now enable the collection of hundreds observations over a few hours individual Previous analyses data for antibiotic tobramycin identified significant (several‐fold), hours‐scale changes...
The anterior cingulate cortex (ACC) is known to be involved in effortful choice, yet its role cost-benefit evaluation of qualitatively different rewards (more/less preferred), beyond magnitude differences (larger/smaller), poorly understood. Selecting between options a decision type commonly faced by humans. Here, we assessed the ACC on task that has primarily been used probe striatal function motivation. Rats were trained stable performance progressive ratio schedule for sucrose pellets and...
Electrochemical aptamer-based sensors support the high-frequency, real-time monitoring of molecules-of-interest in vivo. Achieving this requires methods for correcting sensor drift seen during vivo placements. While correction ensures EAB measurements remain accurate, as progresses it reduces signal-to-noise ratio and precision. Here, we show that enzymatic cleavage sensor's target-recognizing DNA aptamer is a major source signal loss. To demonstrate this, deployed tobramycin-detecting...
Abstract Electrochemical aptamer‐based sensors support the high‐frequency, real‐time monitoring of molecules‐of‐interest in vivo. Achieving this requires methods for correcting sensor drift seen during vivo placements. While correction ensures EAB measurements remain accurate, as progresses it reduces signal‐to‐noise ratio and precision. Here, we show that enzymatic cleavage sensor's target‐recognizing DNA aptamer is a major source signal loss. To demonstrate this, deployed...
Background and Purpose The ability to measure specific molecules at multiple sites within the body simultaneously, with a time resolution of seconds, could greatly advance our understanding drug transport elimination. Experimental Approach As proof‐of‐principle demonstration, here we describe use electrochemical aptamer‐based (EAB) sensors antibiotic vancomycin from plasma (measured in jugular vein) cerebrospinal fluid lateral ventricle) live rats temporal few seconds. Key Results In first...
Electrochemical aptamer-based sensors are the first molecular monitoring technology that support real-time measurements in living body. Electrode placement plays a key role performance of these when they deployed for intravenous measurements.
Metal-Organic FrameworksIn their Research Article (e202319177), Lei Li, Qiang Xu et al. report a metal-organic framework-derived Zr/Ti bimetallic oxide solid solution anchored with Au nanoparticles through crystal engineering and derivation, achieving photocatalytic CO 2 reduction to syngas.
The ability to measure drug, metabolite and biomarker levels in the living body, real time, at high frequency would revolutionize our understand diagnose disease personalize guide its treatment. To this end, Plaxco group has been developing electrochemical aptamer-based (EAB) sensors, a platform technology able molecules body independently of chemical or enzymatic reactivity targets. This enabled development sensors capable measuring both drugs (e.g., antibiotics, chemotherapeutics,...
Abstract Therapeutic drug monitoring (TDM) is a clinical practice involving quantification of plasma concentrations to adjust treatment efficacy and minimize toxicity in patient care. TDM relevant for drugs with narrow therapeutic index, defined as the concentration range over which molecule therapeutically effective without causing significant adverse effects. For index drugs, indirect predictors patient’s metabolism, such age, body mass, or genotype, are insufficient ensure dosing,...
We show that using fast Fourier transform electrochemical impedance spectroscopy (FFT-EIS) to interrogate aptamer-based biosensors leads improved (here better than 2 s) time resolution and calibration-free operation, even when such sensors are deployed in vivo. Because this approach uses the heterogeneous electron transfer rate constant as a means of monitoring target concentration, rather absolute current, it is independent both sensor-to-sensor fabrication variation drift arising due...