A5088506587- Tendon Structure and Treatment
- Tissue Engineering and Regenerative Medicine
- Muscle Physiology and Disorders
- Wound Healing and Treatments
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Pluripotent Stem Cells Research
- Adenosine and Purinergic Signaling
- Laser Applications in Dentistry and Medicine
- Electrospun Nanofibers in Biomedical Applications
- Collagen: Extraction and Characterization
- RNA Interference and Gene Delivery
- Shoulder Injury and Treatment
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Echinoderm biology and ecology
- Silk-based biomaterials and applications
- Bone Tissue Engineering Materials
- Animal Genetics and Reproduction
- Medical and Biological Ozone Research
- Ocular Surface and Contact Lens
- Veterinary Equine Medical Research
- Orthopedic Surgery and Rehabilitation
- Burn Injury Management and Outcomes
- Nanopore and Nanochannel Transport Studies
University of Bari Aldo Moro
2020-2023
University of Padua
2010-2020
Boticário Group Foundation
2009
In-Q-Tel
2007
Collagen-based skin-like scaffolds (CBSS) are promising alternatives to skin grafts repair wounds and injuries. In this work, we propose that the common marine invertebrate sea urchin represents a eco-friendly source of native collagen develop innovative CBSS for injury treatment. Sea food waste after gonad removal was here used extract fibrillar glycosaminoglycan (GAG)-rich produce bilayer (2D + 3D) CBSS. Microstructure, mechanical stability, permeability water proteins, ability exclude...
Mammalian adult stem cells show, in vitro, extensive differentiative ability and may represent a versatile tool for tissue regenerative purposes, even after long-term storage. Multipotent isolated from horse blood have been shown to possess the capacity differentiate into diverse mesenchymal lineages although their full characterization is still at an early stage. The aim of this study was examine effects cryopreservation on stemness characteristics equine peripheral (ePB-MSC). Each sample...
Collagen has become a key-molecule in cell culture studies and the tissue engineering field. Industrially, principal sources of collagen are calf skin bones which, however, could be associated to risks serious disease transmission. In fact, derived from alternative riskless is required, marine organisms among safest recently exploited ones. Sea urchins possess circular area soft surrounding mouth, peristomial membrane (PM), mainly composed by mammalian-like collagen. The PM edible sea urchin...
Skin wound healing includes a system of biological processes, collectively restoring the integrity skin after injury. Healing by second intention refers to repair large and deep wounds where tissue edges cannot be approximated substantial scarring is often observed. The objective this study was evaluate effects mesenchymal stem cells (MSCs) in using surgical model sheep. MSCs are known contribute inflammatory, proliferative, remodeling phases regeneration process rodent models, but data...
Abstract Tendon injuries, degenerative tendinopathies, and overuse tendinitis are common in races horses. Novel therapies aim to restore tendon functionality by means of cell‐based therapy, growth factor delivery, tissue engineering approaches. This study examined the use autologous mesenchymal stromal cells derived from peripheral blood (PB‐MSCs), platelet‐rich plasma (PRP) a combination both for ameliorating experimental lesions on deep digital flexor tendons (DDFT) Bergamasca sheep. In...
The major goal of regenerative medicine is to determine experimental techniques that take maximal advantage reparative processes occur naturally in the animal body. Injection mesenchymal stem cells into core a damaged tendon represents such an approach. Decellularization native tendons as potential targets and seeding protocols are currently under investigation. aim our study was manufacture recellularized biocompatible scaffold from cadaveric tissue for use total or partial injuries....
Platelet-rich plasma (PRP) is known to play a crucial role in skin wound healing, both Human and Veterinary Medicine. Remarkably, until now, no studies have reported PRP treatment subacute full-thickness wounds of the dog. The aim this study was evaluate effects two consecutive applications autologous PRP, with second application after 15 days, 6 dogs showing large wounds. percentage contraction, re-epithelialization healing all treated patients indicated that complications or side effects,...
The mutable collagenous tissue (MCT) of echinoderms possesses biological peculiarities that facilitate native collagen extraction and employment for biomedical applications such as regenerative purposes the treatment skin wounds. Strategies regeneration have been developed dermal substitutes used to cover lesion cell proliferation, although very little is known about application novel matrix obtained from marine collagen. From food waste we isolated eco-friendly collagen, naturally enriched...
Skin wound healing is a complex and dynamic process that aims to restore lesioned tissues. Collagen-based skin substitutes are promising treatment promote by mimicking the native structure. Recently, collagen from marine organisms has gained interest as source for producing biomaterials regenerative strategies. This preliminary study aimed describe application of collagen-based skin-like scaffold (CBSS), manufactured with extracted sea urchin food waste, treat experimental wounds in large...
Evidence indicates that extracellular ATP may have relevant functions in skeletal muscle, even though the physiological role and distribution of specific signaling pathway elements are not well known. The present work shows P2X4 receptor, an ATP-regulated cell membrane channel permeable to Ca2+, is expressed several tissues rat, including muscle. A antibody detected a protein band approximately 60 kDa. Immunofluorescence demonstrated has intracellular localization, confocal analysis revealed...
α-Sarcoglycan is a glycoprotein associated with the dystrophin complex at sarcolemma of skeletal and cardiac muscles. Gene defects in α-sarcoglycan lead to severe muscular dystrophy whose molecular mechanisms are not yet clear. A first insight into function was obtained by finding that it an ATP-binding protein probably confers ability hydrolyse ATP purified [Betto, Senter, Ceoldo, Tarricone, Biral Salviati (1999) J. Biol. Chem. 274, 7907–7912]. In present study, we definitive evidence...
α-Sarcoglycan (Sgca) is a transmembrane glycoprotein of the dystrophin complex located at skeletal and cardiac muscle sarcolemma. Defects in α-sarcoglycan gene ( Sgca) cause severe human-type 2D limb girdle muscular dystrophy. Because Sgca-null mice develop progressive dystrophy similar to human disorder they are valuable animal model for investigating physiopathology disorder. In this study, biochemical functional properties fast-twitch extensor digitorum longus (EDL) slow-twitch soleus...
Besides the presence of somatic stem cells in hair follicles and dermis, epidermis also contains a subpopulation cells, reflecting its high regenerative capacity. However, only limited information concerning epidermis-derived epithelial-like stem/progenitor (EpSCs) is available to date. Nonetheless, this cell type could prove itself useful skin reconstitution after injury. After harvesting from equine epidermis, purified were characterized as EpSCs by means positive expression for CD29,...
To evaluate the efficiency of platelet-rich plasma preparations by means a double centrifugation tube method to obtain canine at concentration least 4 times higher than baseline value and white blood cells not exceeding twice reference range. A complete count was carried out for each sample concentrate. Whole samples were collected from 12 clinically healthy dogs (consenting donors). Blood processed platelet concentrates, which then analyzed flow cytometry haematology system haemogram....
Strain injuries of tendons are the most common orthopedic in athletic subjects, be they equine or human. When tendon is suddenly damaged, an acute inflammatory phase occurs whereas its repetitive overloading may cause chronic injuries. Currently criteria used for grading general and subjective, therefore a reliable method would improvement. The main purpose this study was to assess qualitatively histological pattern Masson trichrome stain healthy injured tendons; indeed, known "paradox"...
The sternomastoid (SM) muscle in rodents presents a peculiar distribution of fiber types with steep gradient from the ventral, superficial, white portion to dorsal, deep, red region, where spindles are restricted. Cross section medial longitudinal third rat SM contains around 10,000 fibers mean diameter 51.28±12.62 (μm +/- SD). Transverse sections stained by Succinate Dehydrogenase (SDH) reaction clearly two distinct regions: dorsal deep encompassing 40% cross area high percentage packed...