Degenerative joint disease (DJD) is a major cause of reduced athletic function and retirement in equine performers. For this reason, regenerative therapies for DJD have gained increasing interest. Platelet-rich plasma (PRP) mesenchymal stem cells (MSCs) were isolated from 6-year-old donor horse. MSCs either used their native state or after chondrogenic induction. In an initial study, 20 horses with naturally occurring the fetlock divided 4 groups injected following: 1) PRP; 2) MSCs; 3) 4)...

Skin wound healing includes a system of biological processes, collectively restoring the integrity skin after injury. Healing by second intention refers to repair large and deep wounds where tissue edges cannot be approximated substantial scarring is often observed. The objective this study was evaluate effects mesenchymal stem cells (MSCs) in using surgical model sheep. MSCs are known contribute inflammatory, proliferative, remodeling phases regeneration process rodent models, but data...

Degenerative joint disease is one of the main causes equine early retirement from pleasure riding or a performance career. The initially triggered by an abnormal loading normal cartilage cartilage. This primary insult accompanied with inflammation, which leads to further progressive degeneration articular and changes in surrounding tissues. Therefore, search for effective treatment, 75 adult horses signs degenerative fetlock were enrolled randomized, multicenter, double-blinded,...

Cell-based therapies, such as treatments with mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) are thought to have beneficial effects on the clinical outcome of orthopedic injuries, but very few animal studies large sample size published so far. Therefore, aim this study was assess safety report allogenic, immature or chondrogenic induced MSCs in combination PRP for treatment degenerative joint disease (DJD) 165 horses. were isolated from a 6-year-old donor horse transplanted...

Poor healing of tendon and ligament lesions often results in early retirement sport horses. Regenerative therapies are therefore being explored as alternative treatment for these injuries. In this study, an intralesional injection was performed with allogeneic tenogenically induced MSCs PRP 5 to 6 days after diagnosis SL (n=68) or SDFT (n=36) lesion. Clinical, lameness ultrasonographic evaluation at 12 weeks. Moreover, a survey 24 months determine how many horses were competing original...

There is a need to improve therapies for osteoarthritis in horses.To assess the efficacy of equine allogeneic chondrogenic-induced mesenchymal stem cells combined with plasma as novel therapy horses.Randomised, double-blinded, placebo-controlled experiment.In 12 healthy horses, was induced metacarpophalangeal joint using an osteochondral fragment-groove model. Five weeks after surgery, horses were randomly assigned either intra-articular injection + (= intervention) or 0.9% saline solution...

Stem-cell therapy represents a promising strategy for the treatment of challenging pathologies, such as large, infected wounds that are unresponsive to conventional therapies. The present study describes clinical application peripheral blood stem cells (PBSCs) four adult Warmblood horses with naturally occurring wounds, which were therapies at least 3 months. A visual assessment was performed, and number wound-healing parameters (granulation tissue, crust formation scar formation) evaluated....

Suspensory ligament injuries are a common injury in sport horses, especially competing dressage horses. Because of the poor healing chronic recalcitrant tendon injuries, this represents major problem rehabilitation horses and often compromises return to initial performance level. Stem cells considered as novel treatment for different pathologies humans. Autologous mesenchymal stem (MSCs) well known their use tendinopathies; however, recent studies report safe allogeneic MSCs orthopedic...

Conventional treatments of osteoarthritis (OA) reduce pain and the inflammatory response but do not repair damaged cartilage. Xenogeneic peripheral blood-derived equine chondrogenically induced mesenchymal stem cells (ciMSC) could thus provide an interesting alternative. Six client-owned dogs with confirmed elbow OA were subjected to a baseline orthopedic examination, pressure plate analysis, general clinical hematological synovial fluid sampling, radiographic their owners completed two...

It has been reported that mesenchymal stem cells (MSCs) have homing capacities and immunomodulating effects after an intravenous injection. However, transplanting MSCs in murine tail veins can result pulmonary reactions even death of the animals. Unfortunately, only a few MSC transplantations large animal species these were performed limited number individuals. To assess safety transplantations, study on 291 recipient horses is here. isolated from peripheral blood (PB) 4-year-old 6-year-old...

Induced pluripotent stem cells (iPSCs) hold great potential not only for human but also veterinary purposes. The equine industry must often deal with health issues concerning muscle and cartilage, where comprehensive regenerative strategies are still missing. In this regard, a open question is whether iPSCs differentiate toward donor cell type influences their differentiation potential. We addressed these questions through an isogenic system of obtained from myogenic mesoangioblasts...

Background: Tendon injuries are very common in horses and jeopardize the athletic performance, due to high risk of reinjury may lead early retirement. The use mesenchymal stem cells for treatment equine tendon disease is widely investigated because their regenerative potential. objective this study investigate safety efficacy allogeneic tenogenic primed (tpMSCs) management tendinitis horses. Methods: A core lesion was surgically induced superficial digital flexor both forelimbs eight After 7...

Background Mesenchymal stem cells provide a valuable treatment option in orthopedic injuries horses. Objectives The aim of this study was to evaluate the hematological, biochemical, immunological and immunomodulatory parameters following intralesional with tenogenic primed equine allogeneic peripheral blood-derived mesenchymal (tpMSCs) client-owned horses naturally occurring superficial digital flexor tendon (SDFT) suspensory ligament (SL) injuries. Methods immunogenicity capacities tpMSCs...

Besides the presence of somatic stem cells in hair follicles and dermis, epidermis also contains a subpopulation cells, reflecting its high regenerative capacity. However, only limited information concerning epidermis-derived epithelial-like stem/progenitor (EpSCs) is available to date. Nonetheless, this cell type could prove itself useful skin reconstitution after injury. After harvesting from equine epidermis, purified were characterized as EpSCs by means positive expression for CD29,...

The use of mesenchymal stem cells (MSCs) for the treatment equine joint disease is widely investigated because their regenerative and immunomodulatory potential. Allogeneic MSCs provide a promising alternative to autologous MSCs, since former are immediately available enable thorough donor screening. However, questions have been raised concerning immunogenic potential allogeneic especially after repeated administration.Current retrospective study assessed cellular humoral immunogenicity ten...

Equine peripheral blood (ePB) can be used as a source of stem cells (SCs) in horses, both for research and practical purposes.A relatively low volume ePB is sufficient the purification expansion SCs.The identification SCs performed by demonstrating presence (CD34, CD90, CD105 CD117) or absence (CD14) specific markers on cell surface means fluorescent staining, followed Fluorescence Activated Cell Sorting (FACS) sorting out desired population entire process SC isolation enrichment from...

Clinical results of regenerative treatments for osteoarthritis are becoming increasingly significant. However, several questions remain UNANSWERED concerning mesenchymal stem cell (MSC) adhesion and incorporation into cartilage.To this end, peripheral blood (PB) MSCs were chondrogenically induced and/or stimulated with pulsed electromagnetic fields (PEMFs) a brief period time just sufficient to prime differentiation. In an organ culture study, PKH26 labelled added at two different densities...

The mammary gland is a highly regenerative organ that can undergo multiple cycles of proliferation, lactation, and involution. Based on the facts (i) stem/progenitor cells (MaSC) are proposed to be driving forces behind growth function (ii) variation exists between mammalian species with regard physiological pathological functioning this organ, we believe studying MaSC from different mammals great comparative interest. Over years, important data has been gathered men mice, although knowledge...

The use of mesenchymal stem cells (MSCs) for the treatment equine tendon disease is widely investigated because their regenerative and immunomodulatory potential. However, questions have been raised concerning immunogenic properties allogeneic MSCs. Therefore, two studies were conducted to assess safety peripheral blood-derived tenogenic primed MSCs (tpMSCs). objective was evaluate if a single repeated tpMSC administration induced cellular humoral immune response in horses suffering from...