A5090891674- Optical Imaging and Spectroscopy Techniques
- Thermoregulation and physiological responses
- Traumatic Brain Injury and Neurovascular Disturbances
- Infrared Thermography in Medicine
- Photoacoustic and Ultrasonic Imaging
- Advanced MRI Techniques and Applications
- Thermal Regulation in Medicine
- MRI in cancer diagnosis
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Functional Brain Connectivity Studies
- Traumatic Brain Injury Research
- Cardiac Arrest and Resuscitation
- Advanced Fluorescence Microscopy Techniques
- Mesenchymal stem cell research
- Analytical Chemistry and Sensors
- Infant Nutrition and Health
- Angiogenesis and VEGF in Cancer
- Cardiac Imaging and Diagnostics
- Bluetooth and Wireless Communication Technologies
- Spaceflight effects on biology
- Medical Image Segmentation Techniques
- Smart Agriculture and AI
- Cell Image Analysis Techniques
- Viral Infectious Diseases and Gene Expression in Insects
Johns Hopkins University
2015-2024
Discovery Institute
2023
Johns Hopkins Medicine
2015-2023
University of Moratuwa
2009
Abstract Neurovascular coupling, cerebrovascular remodeling and hemodynamic changes are critical to brain function, dysregulated in neuropathologies such as tumors. Interrogating these phenomena freely behaving animals requires a portable microscope with multiple optical contrast mechanisms. Therefore, we developed miniaturized with: fluorescence (FL) channel for imaging neural activity (e.g., GCaMP) or fluorescent cancer cells 9L-GFP); an intrinsic signal (IOS) hemoglobin absorption (i.e.,...
Cloud computing offers cost efficiency and scalability but introduces significant security concerns related to data control. Cryptography addresses these by ensuring confidentiality, integrity, authenticity, availability . This research document provides an in-depth analysis of cryptographic techniques in cloud computing, including symmetric asymmetric encryption, homomorphic post-quantum cryptography. It critically evaluates the strengths limitations current approaches, particularly key...
Laser speckle contrast imaging (LSCI) is a full field optical technique, capable of blood flow without the introduction any exogenous dyes. Spatial and temporal resolution in LSCI images depend on how pixels are chosen from raw image stack for processing. However, all processing schemes based isotropic treatment spatial neighborhood about each pixel, restricting further improvement spatiotemporal quality. We present novel scheme where anisotropic, that is, restricted along specific direction...
Necrotizing enterocolitis (NEC) is the most prevalent gastrointestinal emergency in premature infants and characterized by a dysfunctional gut microcirculation. Therefore, there dire need for vivo methods to characterize NEC-induced changes structure function of microcirculation, that is, its vascular phenotype. Since imaging are often slow employ single-contrast mechanism, we developed rapid multicontrast technique novel analyses pipeline phenotyping microcirculation.Using an experimental...
Recent advances in low-power wireless-capable system-on-chips (SoCs) have accelerated diverse Internet of Things (IoT) applications, encompassing wearables, asset monitoring, and more. Concurrently, the field neuroimaging has experienced escalating demand for lightweight, untethered, systems capable imaging small animals. This article explores feasibility using a monitoring system as basis new architecture fluorescence hemodynamic contrast-based wireless functional imaging. The core hinges...
Imaging the brain in animal models enables scientists to unravel new biological insights. Despite critical advancements recent years, most laboratory imaging techniques comprise of bulky bench top apparatus that require imaged animals be anesthetized and immobilized. Thus, are their non-native state severely restricting scope behavioral experiments. To address this gap, we report a miniaturized microscope can mounted on rat's head for awake unrestrained conditions. The uses laser speckle...
Tissue-engineered scaffolds are a powerful means of healing craniofacial bone defects arising from trauma or disease. Murine models critical-sized especially useful in understanding the role microenvironmental factors such as vascularization on regeneration. Here, we demonstrate capability novel multimodality imaging platform capable acquiring vivo images microvascular architecture, blood flow, and tracer/cell tracking via intrinsic optical signaling (IOS), laser speckle contrast (LSC),...
Abstract Abnormal tumor hemodynamics are a critical determinant of tumor’s microenvironment (TME), and profoundly affect drug delivery, therapeutic efficacy the emergence radio-resistance. Since multiple hemodynamic variables can simultaneously exhibit transient spatiotemporally heterogeneous behavior, there is an exigent need for analysis tools that employ to characterize anomalous within TME. To address this, we developed new toolkit called HemoSYS quantifying landscape angiogenic...
Imaging of cortical vasculature in rodents and its dynamic response to experimental conditions, yield valuable information regarding the underlying physiological mechanisms neuronal activity. Laser Speckle Contrast (LSCI) facilitates both enhanced micro-vessel visualization blood flow information. The current state equipment used: imaging systems, external lighting as well recording apparatus constrain modern bench-top experiments with anaesthetized rodents. We describe an system that...
The cranial window preparation provides optical access to the rodent brain for high-resolution in vivo imaging. Two types of windows are commonly employed, namely open-skull and thinned-skull window. Chronic laser speckle contrast imaging (LSCI) through permits characterization neurovascular morphology blood flow changes over days or weeks. However, effects type their long-term stability LSCI have not been studied. Here we systematically characterize effect each on monitoring with two...
Angiogenesis is a key factor in bone healing that allows the delivery of oxygen, nutrients, inflammatory and precursor cells to defect site. It induces dramatic structural functional remodeling vasculature during first 4 weeks healing. Although recent advances optical imaging have elucidated vivo relationship between angiogenesis osteogenesis calvarial model, these efforts were mostly limited bone. Therefore, better characterize changes vascular function (i.e. blood flow, oxygenation, etc.)...
The field of systems neuroscience strives to comprehend the intricate links between brain activity and behavior, with goal understanding neurological disorders targeted interventions, notably in feedback-based therapies like Deep Brain Stimulation (DBS). However, challenges persist correlating neuroimaging data especially freely behaving animals across different imaging modalities. In last decade, deep learning has emerged as a crucial tool for analyzing image enabling correlation or...
Different brain regions exhibit complex information processing even at rest. Therefore, assessing temporal correlations between permits task-free visualization of their 'resting state connectivity'. Although functional MRI (fMRI) is widely used for mapping resting connectivity in the human brain, it not well suited 'microvascular scale' imaging rodents because its limited spatial resolution. Moreover, co-registered cerebral blood flow (CBF) and total hemoglobin (HbT) data are often...
Assessing intravascular blood oxygen saturation (SO2) is crucial for characterizing in vivo microenvironmental changes preclinical models of injury and disease. However, most conventional optical imaging techniques mapping SO2 assume or compute a single value the path-length tissue. This especially detrimental when experimental disease wound healing that are characterized by vascular tissue remodeling. Therefore, to circumvent this limitation we developed an technique utilizes...