A5082010431- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Virology and Viral Diseases
- Malaria Research and Control
- Metabolomics and Mass Spectrometry Studies
- Endoplasmic Reticulum Stress and Disease
- HIV/AIDS Research and Interventions
- Viral gastroenteritis research and epidemiology
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- Leptospirosis research and findings
- Data-Driven Disease Surveillance
- Marine and coastal ecosystems
- Microbial Community Ecology and Physiology
- Bacillus and Francisella bacterial research
- HIV, TB, and STIs Epidemiology
- Bat Biology and Ecology Studies
- Yersinia bacterium, plague, ectoparasites research
- HIV Research and Treatment
Oak Ridge Institute for Science and Education
2024
Oak Ridge Associated Universities
2024
Florida College
2022-2023
University of Florida
2016-2023
University of Florida Health Science Center
2023
University of Florida Health
2023
UF Health Shands Hospital
2023
University of Goma
2022
University of California, Berkeley
2022
Tufts University
2022
Zikavirus (ZIKV) is an emerging viral pathogen that continues to spread throughout different regions of the world. Herein we report a case provides further evidence ZIKV transmission can occur through breastfeeding by providing detailed clinical, genomic, and virological case-based description.
Although over 40 type 1 diabetes (T1D) risk loci have been mapped in humans, the causative genes and variants for T1D are largely unknown. Here, we investigated a candidate gene 21q22.3 locus-UBASH3A, which is primarily expressed T cells where it thought to play redundant role. Genetic UBASH3A shown be associated with several autoimmune diseases addition T1D. However, molecular mechanism underlying these genetic associations unresolved. Our study reveals previously unrecognized role of human...
The spread of cholera in the midst an epidemic is largely driven by direct transmission from person to person, although it well-recognized that Vibrio cholerae also capable growth and long-term survival aquatic ecosystems. While prior studies have shown reservoirs are important persistence disease on Indian subcontinent, epidemiological view postulating locally evolving environmental V. contributes outbreaks outside Asia remains debated. single-source introduction toxigenic O1 Haiti, one...
The Mississippi River (MR) serves as the primary source of freshwater and nutrients to northern Gulf Mexico (nGOM). Whether this input also enriches microbial diversity MR plume migrates mixes with nGOM central question addressed herein. Specifically, in study physicochemical properties planktonic community composition was determined using iTag sequencing 16S rRNA genes 23 samples collected along a salinity (and nutrient) gradient from mouth MR, plume, canyon, at Deepwater Horizon wellhead...
The TCR-CD3 complex is a multicomponent membrane receptor, the expression of which tightly regulated in thymocytes, as well mature T cells both at steady state and upon stimulation. In this study, we report novel roles for UBASH3A synthesis turnover. negative regulator cell function plays broad role autoimmunity. We show that modulation levels unstimulated Jurkat leads to altered amounts total cellular CD3 chains cell-surface complexes; contrast, does not affect level CD28, an important...
In the context of recent arbovirus epidemics, questions about frequency simultaneous infection patients with different species have been raised. 2014, a major Chikungunya virus (CHIKV) epidemic impacted Caribbean and South America. As part ongoing screening schoolchildren presenting acute undifferentiated febrile illness in rural Haiti, we used RT-PCR to identify CHIKV infections 82 100 children this diagnosis during May—August 2014. Among these, eight were infected second arbovirus: six...
The aquatic communities found within the water filled leaves of pitcher plant, Sarracenia purpurea, have a simple trophic structure providing an ideal system to study microscale interactions between protozoan predators and their bacterial prey. In this study, replicate were maintained with without presence bactivorous protozoan, Colpoda steinii, determine effects grazing on microbial communities. Changes in (Archaea Bacteria) community assessed using iTag sequencing 16S rRNA genes. similar...
ABSTRACT Complete genome sequences were obtained for Zika viruses isolated from the breast milk of a Venezuelan patient and her child, who was exclusively breastfeeding at time. These are first to be reported presumptive autochthonous postnatal transmission case mother child in Venezuela.
Zikavirus (ZIKV) and Chikungunyavirus (CHIKV) can share the same mosquito vector, co-infections by these viruses occur in humans. While infections with commonalities, CHIKV is unique causing arthritis arthralgias that may persist for a year or more. These are commonly diagnosed RT-PCR-based methods during acute phase of infection. Even high specificity sensitivity characteristic PCR, false negatives occur, highlighting need additional diagnostic confirmation.On her return to USA, traveller...
Abstract Cholera causes substantial illness and death in Africa. We analyzed 24 toxigenic Vibrio cholerae O1 strains isolated 2015–2017 from patients the Great Lakes region of Democratic Republic Congo. Strains originating southern Asia appeared to be part T10 introduction event eastern identified 2 main strain lineages, most recently a lineage corresponding sequence type 515, V. cluster previously reported Lake Kivu region. In 41% fecal samples cholera patients, we also novel ICP1...
Toxigenic Vibrio cholerae O1 serotype Ogawa was introduced involuntarily into Haiti in October 2010, and virtually all of the clinical strains isolated during first 5 years epidemic were Ogawa. Inaba identified intermittently prior to 2015, with diverse mutations resulting a common phenotype. In percentage infections due began rapidly increase, supplanting as dominant subsequent 4 years. We investigated molecular basis switch confirmed that had same level mRNA expression wbeT genes, well...
HIV-1 subtype C is the most abundant strain of infections worldwide and was found in first known patients diagnosed with HIV/AIDS Bulgaria 1986. However, there limited information on molecular-epidemiological characteristics this epidemic country. In study, we analyze evolutionary history introduction dissemination using global phylogenetic analysis, Bayesian coalescent-based approach, molecular clock methods. All available samples from individuals between 1986 2017 were analyzed. Men women...
Vibrio mimicus caused a seafood-associated outbreak in Florida, USA, which 4 of 6 case-patients were hospitalized; 1 required intensive care for severe diarrhea. Strains ctx-negative but carried genes other virulence determinants (hemolysin, proteases, and types I-IV VI secretion systems). Cholera toxin-negative bacterial strains can cause cholera-like disease.
Bats are natural reservoirs of coronaviruses and other viruses with zoonotic potential. Florida has indigenous non-migratory populations Brazilian free-tailed bats (Tadarida brasiliensis) that mostly roost in colonies artificial structures. Unlike their counterparts Brazil Mexico, the harbored by have been underexplored. We report detection an alphacoronavirus RNA-dependent RNA polymerase (RdRp) gene sequence feces two 19 different T. brasiliensis were capture/release had evaluated for...
Cholera is an acute watery, diarrheal disease that causes high rates of morbidity and mortality without treatment. Early detection the etiologic agent toxigenic Vibrio cholerae important to mobilize treatment mitigate outbreaks. Monoclonal antibody (mAb) based rapid diagnostic tests (RDTs) enable early in settings laboratory capacity. However, odds RDT testing positive are reduced by nearly 90% when common virulent bacteriophage ICP1 present. We hypothesize adding a mAb for common, specific,...
causes anthrax through virulence factors encoded on two plasmids. However, non-
Bacteriophage predation of toxigenic Vibrio cholerae O1 (the causative agent cholera) has been linked with seasonal patterns disease and clinical response to infection in humans 1-4 . However, we still lack a clear demonstration how the interplay between bacteria bacteriophage can influence shifts strain populations. We analyzed V. isolated from patients Great Lakes, cholera endemic region Democratic Republic Congo (DRC), 2013-2017. Bayesian phylogeography shows that all strains derived East...
ABSTRACT Cholera is an acute watery diarrheal disease that causes high rates of morbidity and mortality without treatment. Early detection the etiologic agent toxigenic Vibrio cholerae important to mobilize treatment mitigate outbreaks. Monoclonal antibody (mAb) based rapid diagnostic tests (RDTs) enable early in settings laboratory capacity. However, odds RDT testing positive are reduced by nearly 90% when common virulent bacteriophage ICP1 present. We hypothesize adding a mAb for common,...
Abstract Background Shotgun sequencing-based metagenomics is a useful approach to profiling microbiomes in environmental and patient samples. In clinical setting, metagenomic techniques have the advantage of identifying organisms, which cannot be readily cultured or confirmed by other techniques. We developed clinical-grade, streamlined metagenomics-based pipeline that includes regulatory compliant method considerations, such as an internal control followed machine-based learning (ML)...