A5101475499- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Child Nutrition and Water Access
- Genomics, phytochemicals, and oxidative stress
- Viral gastroenteritis research and epidemiology
- Aquaculture disease management and microbiota
- Hepatitis B Virus Studies
- Free Radicals and Antioxidants
- Hepatitis C virus research
- Hemoglobinopathies and Related Disorders
- Gut microbiota and health
- Curcumin's Biomedical Applications
- Influenza Virus Research Studies
- COVID-19 and Mental Health
- Biosensors and Analytical Detection
- Animal Diversity and Health Studies
- Cassava research and cyanide
- Fisheries and Aquaculture Studies
- COVID-19 epidemiological studies
- Piperaceae Chemical and Biological Studies
- Neuroendocrine Tumor Research Advances
- Synthesis and biological activity
- MicroRNA in disease regulation
University of Florida
2020-2025
University of Florida Health
2020-2024
International Centre for Diarrhoeal Disease Research
2015-2022
University of Cambridge
2021
Manipal Academy of Higher Education
2021
University of Delhi
2021
Marche Polytechnic University
2016-2018
Massachusetts General Hospital
2015
Chittagong Medical College
1970
Despite an increasingly detailed picture of the molecular mechanisms bacteriophage (phage)-bacterial interactions, we lack understanding how these interactions evolve and impact disease within patients. In this work, report a year-long, nationwide study diarrheal patients in Bangladesh. Among cholera patients, quantified
Vibrio cholerae is the cause of cholera, a severe watery diarrhea. Protection against cholera serogroup specific. Serogroup specificity defined by O-specific polysaccharide (OSP) component lipopolysaccharide (LPS).Here we describe conjugate vaccine for prepared via squaric acid chemistry from OSP V. O1 Inaba strain PIC018 and recombinant heavy chain fragment tetanus toxin (OSP:rTTHc). We assessed range doses based on content (10-50 μg), compositions varying molar loading ratio to rTTHc (3:1,...
Recognizing cholera cases early, especially in the initial phase of an outbreak and areas where has not previously circulated, is a high public health priority. Laboratory capacity such settings often limited. To address this, we have developed rapid diagnostic test (RDT) termed Cholkit that based on immunochromatographic lateral flow assay for diagnosis using stool. contains monoclonal antibody (ICL-33) to O-specific polysaccharide (OSP) component V. cholerae O1 lipopolysaccharide,...
Infections from Shigella spp. and Enteroinvasive Escherichia coli (EIEC) are considered leading causes of symptomatic diarrheal disease, globally. However, there is a paucity case-control studies Caribbean nations to guide regional public health priorities interventions. A study was conducted within larger cross-sectional healthcare in Haïti. Participant households were identified using geospatially randomized method; families with children under 5 years consented enrolled. Rectal swabs...
Cholera remains a substantial health burden in Asia and Africa particularly resource poor settings. The standard procedures to identify the etiological organism V. cholerae are isolation from microbiological culture stool as well Polymerase Chain Reaction (PCR). Both processes highly lab oriented, labor extensive, time consuming, expensive. In an effort control for outbreaks epidemics; effective, convenient, quick relatively less expensive detection method is imperative, without compromising...
Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhea in children low- and middle-income countries (LMICs). However, large-scale pathogen burden studies have identified ETEC the guts both symptomatic patients controls. The factors that influence this balance are poorly understood, but it postulated gut microbiome may play a role either resistance or progression to disease. In study, we profiled microbiomes adults from Bangladesh who were asymptomatically symptomatically...
Novel adenovirus genotypes are associated with outbreaks of disease, such as acute gastroenteritis, renal upper respiratory tract infection and keratoconjunctivitis. Here, we identify novel variant in children coinfected enterotoxigenic Escherichia coli, Bangladesh. Metagenomic sequencing stool was performed whole genomes were extracted. A species D virus, designated genotype 90 (P33H27F67) identified, the partial genome a putative recombinant B virus recovered. Furthermore, enteric types...
A fundamental, clinical, and scientific concern is how lytic bacteriophage, as well antibiotics, impact diagnostic positivity. Cholera was chosen a model disease to investigate this important question, because cholera outbreaks enable large enrollment, field methods are established, the predatory relationship between bacteriophage etiologic agent Vibrio cholerae share commonalities across bacterial taxa. Patients with diarrheal were enrolled at two remote hospitals in Bangladesh.
It was reported recently that allyl isothiocyanate (AITC) could inhibit various types of cancer cell growth. In the present study, we further investigated whether AITC growth human breast cells. Unexpectedly, found did not inhibit, rather slightly promoted, proliferation MDA-MB-231 cells, although it have inhibitory effect on MCF-7 Cytofluorimetric analysis revealed (10 µM) induce apoptosis and cycle arrest in addition, significantly (p < 0.05) increased expression BCL-2 mTOR genes...
A study was performed for five months to assess the microbiological quality of dried Baim (Mastacembelus armatus) in Sylhet region Bangladesh.A total 45 samples were randomly collected on monthly basis from three different sources region; one producer (drying yard Lamagaji) and others retail market (Bandar Bazar) control (prepared laboratory).The mean plate count (TPC) producer, observed 6.20 ± 0.72 ×10 5 , 9.64 1.58 1.61 1.06×10 cfu/ g, respectively, whereas, average fungal (TFC) estimated...
Abstract Background CEACAM1, a valuable biomarker for several cancers, have remained unexplored up to the present in laryngeal squamous cell carcinoma (LSCC). We aimed examine CEACAM1 expression and evaluate its combinational clinical significance diagnosis or prognosis treatment decision making LSCC. Methods was assessed by immunohistochemistry 54 LSCCs correlation with histopathological features. Results CEACAM subtype 1 (CEACAM1) positive 50% of cases. No significant difference observed...
Transfusion transmitted infections have remained a major deterrent to public health, particularly among the patients with transfusion-dependent Beta thalassemia in developing countries. Although proper donor selection through adoption of WHO-advised infection panel has lowered rate infections, multi-transfused are not free risk. In this study, we screened 148 determine frequency Hepatitis C Virus (HCV), B (HBV) and Human Immunodeficiency (HIV) using ELISA method. Among them, infected cases...
Abstract Despite an increasingly detailed picture of the molecular mechanisms phage-bacterial interactions, we lack understanding how these interactions evolve and impact disease within patients. Here report a year-long, nation-wide study diarrheal patients in Bangladesh. Among cholera patients, quantified Vibrio cholerae (prey) its virulent phages (predators) using metagenomics quantitative PCR, while accounting for antibiotic exposure mass spectrometry. Virulent phage (ICP1) antibiotics...
Cholera is an acute watery, diarrheal disease that causes high rates of morbidity and mortality without treatment. Early detection the etiologic agent toxigenic Vibrio cholerae important to mobilize treatment mitigate outbreaks. Monoclonal antibody (mAb) based rapid diagnostic tests (RDTs) enable early in settings laboratory capacity. However, odds RDT testing positive are reduced by nearly 90% when common virulent bacteriophage ICP1 present. We hypothesize adding a mAb for common, specific,...
ABSTRACT Introduction Cholera rapid diagnostic tests (RDTs) are vulnerable to virulent bacteriophage predation. We hypothesized that an enhanced cholera RDT detects the common ICP1 might serve as a proxy for pathogen detection. previously developed monoclonal antibody (mAb) major capsid protein. Our objective herein was design and assemble first-of-its-kind both bacterial ( Vibrio cholerae ) associated (ICP1). Method Candidate mAbs were expanded increase options evaluated by immunological...
Cholera is a diarrhoeal disease caused by
ABSTRACT Cholera rapid diagnostic tests (RDTs) are vulnerable to virulent bacteriophage predation. We hypothesized that an enhanced cholera RDT detects the common ICP1 might serve as a proxy for pathogen detection. previously developed monoclonal antibody (mAb) major capsid protein. Our objective was design and assemble first-of-its-kind both bacterial ( Vibrio cholerae ) associated (ICP1). Candidate mAbs were expanded increase options evaluated by immunological assays (ELISA; western blot)....
To identify risk factors associated with symptoms of anxiety, depression, and obsessive-compulsive disorder (OCD) among children during the 1st year COVID-19 pandemic.A longitudinal study three cross-sectional timepoints [April 2020 (n = 273), October 180), April 2021 116)] was conducted at a K-12 public school in Florida. Infection sero-positivity for SARS-CoV-2 determined by molecular serologic approaches. Adjusted odds ratios using mixed effect logistic regression models symptom-derived...
ABSTRACT Background A fundamental clinical and scientific concern is how lytic bacteriophage, as well antibiotics, impact diagnostic positivity. Methods Cholera was chosen a model disease to investigate this important question. Patients with diarrheal were enrolled at two remote hospitals in Bangladesh. Diagnostic performance assessed function of bacteriophage detection, exposure the first-line antibiotic azithromycin detected by mass spectrometry. Results Among samples positive nanoliter...
Abstract Transfusion transmitted infections (TTIs) have remained a major deterrent to public health, particularly among the patients with transfusion-dependent Beta thalassemia in developing countries. Although proper donor selection through adoption of WHO-advised infection panel has lowered rate infections, multi-transfused are not free risk. The present study screened 148 determine frequency HCV, HBV and HIV using ELISA method. Among these patients, infected cases were 13.51%, 3.37% 0%,...
After three years with no confirmed cholera cases in Haiti, an outbreak of Vibrio cholerae O1 emerged October 2022. Levels pre-existing antibodies provide estimate prior immunologic exposure, reveal potentially relevant immune responses, and set a baseline for future serosurveillance. We analyzed dried blood spots collected 2021 from population-weighted representative cross-sectional serosurvey two communes the Ouest Department Haiti. found lower levels circulating IgG IgA against V....