A5057133188- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Gene Regulatory Network Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Organic Light-Emitting Diodes Research
- Enzyme Catalysis and Immobilization
- CRISPR and Genetic Engineering
- Photochromic and Fluorescence Chemistry
- Luminescence and Fluorescent Materials
- Bacterial Genetics and Biotechnology
- Microbial Natural Products and Biosynthesis
- Synthesis and biological activity
- Microbial Community Ecology and Physiology
- Organophosphorus compounds synthesis
- Radioactivity and Radon Measurements
- Genetics, Bioinformatics, and Biomedical Research
- Biofuel production and bioconversion
- Asymmetric Hydrogenation and Catalysis
- Radioactive contamination and transfer
- Bacteriophages and microbial interactions
- Advanced Synthetic Organic Chemistry
- Bioactive Compounds and Antitumor Agents
- Polydiacetylene-based materials and applications
- Plant biochemistry and biosynthesis
- Bioinformatics and Genomic Networks
University of Georgia
2021-2025
North China University of Science and Technology
2024
Zhejiang Center for Disease Control and Prevention
2024
Liaoning Shihua University
2019
A billion-clone library of filamentous phage with different surface structures ('landscapes') was generated by fusing random octapeptides to the N-terminus all 4000 copies major coat protein. Such a 'landscape library' might include clones exhibiting emergent properties that inhere in entire architecture, not peptides themselves. Because diverse landscapes are displayed on viable phage, they can be surveyed for exceedingly rare functions using microbiological selection methods. Clones...
By combining protein engineering and synthetic biology strategies, the novel de novo biosynthesis of 4,6-dihydroxycoumarin from glycerol was realized in Escherichia coli for first time.
ABSTRACT Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose carbon source. This phenomenon, known overflow metabolism, has negative impacts on cell and protein expression, also causes loss during biosynthesis most microbial production scenarios. In this study, we regarded the “waste” metabolite useful metabolism indicator, constructed an biosensor to monitor change of concentration converted signal into various regulation outputs....
To achieve low-carbon bioeconomy, we established a carbon-efficient bioproduction approach by fine-tuning non-glycolysis pathway, which reduces CO 2 emission and balances the acetyl-CoA redox supply from sugar breakdown to pathway demand.
Transcriptional factors play a crucial role in regulating cellular functions. Understanding and altering the dynamic behavior of transcriptional factor-based biosensors will expand our knowledge investigating biomolecular interactions facilitating biosynthetic applications. In this study, we characterized engineered TrpR-based tryptophan repressor system Escherichia coli. We found that reconstructed TrpR1-PtrpO1 biosensor exhibited low basal expression narrow range presence or its analogue...
Transcription factor (TF)-based biosensors have arisen as powerful tools in the advancement of metabolic engineering. However, with emergence numerous bioproduction targets, variety applicable TF-based remains severely limited. In this study, we investigated and engineered an 1,2-propanediol (1,2-PD)-responsive transcription activator, PocR, from Salmonella typhimurium to enrich current biosensor repertoire. Heterologous characterization PocR E. coli revealed a significantly limited...
Transcriptional factors-based biosensors are commonly used in metabolic engineering for inducible control of gene expression and related applications such as high-throughput screening dynamic pathway regulations. Mining novel transcriptional factors is essential expanding the usability these toolsets. Here, we report identification, characterization, a phenolic acid responsive regulator PadR from Bacillus amyloliquefaciens (BaPadR). This BaPadR-based biosensor system showed unique ligand...
Recent advances in Large Language Models (LLMs) have presented new opportunities for integrating Artificial General Intelligence (AGI) into biological research and education. This study evaluated the capabilities of leading LLMs, including GPT-4, GPT-3.5, PaLM2, Claude2, SenseNova, answering conceptual biology questions. The models were tested on a 108-question multiple-choice exam covering topics molecular biology, techniques, metabolic engineering, synthetic biology. Among models, GPT-4...
Fluorescent materials have been widely used in the field of anti-counterfeiting, but traditional fluorescent patterns are static by design, which only offer a limited level security for anti-counterfeiting encryption. Herein, we provide novel dynamic strategy constructing flexible, long afterglow film with Bi-activated Sr3Y2Ge3O12 phosphors. Theoretical calculation indicates that garnet matrix suitable energy bands shows high adaptability Bi ions activator to produce emission. Doping into...
The regioselective hydroamination of diphenylphosphorylallenes has been developed using secondary amines as the amination reagents. This reaction protocol could be realized under ligand and transition metal-free conditions at room temperature is tolerated to a diverse range amine substrates, affording series β-aminophosphine oxides in good yields after reduction enamine intermediates.