A5100703960- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Enzyme Catalysis and Immobilization
- Gene Regulatory Network Analysis
- Pesticide Exposure and Toxicity
- Immune Response and Inflammation
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Insect and Pesticide Research
- Biofuel production and bioconversion
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Immune cells in cancer
- Biotin and Related Studies
- Pesticide and Herbicide Environmental Studies
- Plant biochemistry and biosynthesis
- Genetic diversity and population structure
- Molecular Junctions and Nanostructures
- Microbial Natural Products and Biosynthesis
- Immunotherapy and Immune Responses
- Escherichia coli research studies
- Biochemical and biochemical processes
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
Shaanxi University of Chinese Medicine
2025
University of Chinese Academy of Sciences
2016-2024
Shandong Agricultural University
2008-2024
Northwestern University
2024
Chinese University of Hong Kong
2024
University of Hong Kong
2024
National Institute for Radiological Protection
2020-2023
University of Georgia
2017-2023
Hebei North University
2015-2023
Qufu Normal University
2021-2022
Significance The bromodomain and extraterminal domain (BET) proteins regulate transcription of subset-specifying genes during lineage-specific T-helper-cell differentiation in adaptor immunity are also implicated inflammatory disorders. available pan-BET inhibitors such as JQ1 indiscriminately block the tandem bromodomains (BD1 BD2) BET proteins, broadly render different Th subsets, have limited therapeutic potential. Here we report a small molecule, MS402, that can selectively inhibit BD1...
Writing artificial logic and dynamic function into complex cellular background to achieve desired phenotypes or improved outputs calls for the development of new genetic tools as well their innovative use. In this study, we present a sensor-regulator RNAi-based bifunctional control network that can provide simultaneous upregulation downregulation metabolism engineered biosynthesis. The promoter-regulator-mediated its transduced through RNAi are systematically verified characterized. We apply...
A near-infrared-driven photoelectrochemical aptasensor was developed as a new method for the detection of breast cancer cell MCF-7. The upconversion nanoparticles and TiO2/CdTe heterostructure were combined to prepare film electrode, high-affinity aptamer AS1411 conjugated electrode recognize MCF-7 cells. In this fabrication, transferred near-infrared light visible light, which could excite semiconductor enhance current response. As result, revealed good sensitivity specificity with...
The regulation of macrophage orientation pathological conditions is important but still incompletely understood. Here, we show that IL-10 and Rag1 double knockout mice spontaneously develop colitis with dominant M1 phenotype, suggesting regulates in inflammation. We demonstrate stimulation induced miR-146b expression, the expression was impaired deficient macrophages. Our data targets IRF5, resulting activation. Furthermore, developed intestinal inflammation enhanced polarization. Finally,...
The duck hepatitis A virus type 1 (DHAV-1) causes rapid death in ducklings by triggering a severe cytokine storm. Pyroptosis is an inflammatory form of programmed cell that directly related to increase pro-inflammatory levels. Only few studies have explored the mechanisms underlying pyroptosis virus-infected avian cells. In this study, we established infection model vitro infecting embryo fibroblasts (DEFs) with virulent DHAV-1 LY0801 strain. induced DEFs activating gasdermin E (GSDME)...
The aged kidney is susceptible to acute injury due presumably its decreased ability handle additional challenges, such as endoplasmic reticulum (ER) stress. This was tested by giving tunicamycin, an ER stress inducer, either old or young mice. Injection of high dose caused renal failure in mice, not Moreover, injection low resulted severe damage confirming the increased susceptibility There existed abnormality response kinetics kidney, characterized a loss XBP-1 splicing and PERK-eIF2α...
To achieve low-carbon bioeconomy, we established a carbon-efficient bioproduction approach by fine-tuning non-glycolysis pathway, which reduces CO 2 emission and balances the acetyl-CoA redox supply from sugar breakdown to pathway demand.
Abstract The RNA-guided CRISPR-associated Cas9 proteins have been widely applied in programmable genome recombination, base editing or gene regulation both prokaryotes and eukaryotes. SpCas9 from Streptococcus pyogenes is the most extensively engineered with robust manifold functionalities. However, one inherent limitation of its stringent 5′-NGG-3′ PAM requirement that significantly restricts DNA target range. Here, to repurpose as a universal repressor, we generate screen variants...
Abstract Background H9N2 virus is mainly transmitted through the respiratory mucosal pathway, so immunity considered to play a good role in controlling avian influenza infection. It commonly accepted that no adequate achieved by inactivated vaccines, which was widely used prevent and control Thus, an improved vaccine induce both systemic urgently required outbreaks poultry farms. Methods In this study, we constructed novel Lactococcus lactis ( L. ) strain expressing recombinant fusion...
Vanillyl alcohol is a phenolic and used as flavoring agent in foods beverages. In this paper, we propose novel artificial pathway for microbial production of vanillyl from simple carbon sources. The extends 4-hydroxybenzoic acid (4-HBA), needs only three heterologous enzymes, p-hydroxybenzoate hydroxylase (PobA), carboxylic reductase (CAR) caffeate O-methyltransferase (COMT). First, examined the promiscuous activity COMT toward 3,4-dihydroxybenzyl found kcat value 0.097 s-1. Meanwhile,...
Controllable methylenation using renewable ethylene glycol as the methylene source has been developed for introduction of one or two building blocks.
Transcriptional factors play a crucial role in regulating cellular functions. Understanding and altering the dynamic behavior of transcriptional factor-based biosensors will expand our knowledge investigating biomolecular interactions facilitating biosynthetic applications. In this study, we characterized engineered TrpR-based tryptophan repressor system Escherichia coli. We found that reconstructed TrpR1-PtrpO1 biosensor exhibited low basal expression narrow range presence or its analogue...
Abstract 4-Hydroxyphenylacetate 3-hydroxylase (EcHpaB) from Escherichia coli is capable of efficient ortho -hydroxylation a wide range phenolic compounds and demonstrates great potential for broad chemoenzymatic applications. To understand the structural mechanistic basis its catalytic versatility, we elucidated crystal structure EcHpaB by X-ray crystallography, which revealed unique loop covering active site. We further performed mutagenesis studies this to probe role in substrate...