A5039820867- RNA Research and Splicing
- Ovarian cancer diagnosis and treatment
- bioluminescence and chemiluminescence research
- CRISPR and Genetic Engineering
- Metabolism, Diabetes, and Cancer
- Craniofacial Disorders and Treatments
- Cleft Lip and Palate Research
- RNA modifications and cancer
- Pancreatic function and diabetes
- PARP inhibition in cancer therapy
- Angiogenesis and VEGF in Cancer
- Kruppel-like factors research
- Cancer-related Molecular Pathways
- Growth Hormone and Insulin-like Growth Factors
- BRCA gene mutations in cancer
- Cancer, Lipids, and Metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
- interferon and immune responses
- PI3K/AKT/mTOR signaling in cancer
- Lipid metabolism and disorders
- RNA regulation and disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neurogenetic and Muscular Disorders Research
- MicroRNA in disease regulation
- Genomic variations and chromosomal abnormalities
Institute for Health Metrics and Evaluation
2025
McGill University Health Centre
2019-2024
McGill University
2008-2020
Jewish General Hospital
2008-2017
Université de Montréal
1997-2006
Hôpital Notre-Dame
2002
Centre Hospitalier de l’Université de Montréal
2002
Hôpital Maisonneuve-Rosemont
1997
Phosphatase and Tensin homolog (PTEN) is a tumor suppressor gene. Loss of its function the most frequent genetic alteration in endometrioid endometrial cancers (70-80%) high grade tumors (90%). We assessed sensitivity cancer cell lines to PARP inhibitors (olaparib BMN-673) PI3K inhibitor (BKM-120), alone or combination, context their PTEN mutation status. also highlighted direct pathway linking DNA repair.Using cellular models with known status, we evaluated homologous recombination (HR)...
C-Reactive protein (CRP), the classic acute-phase reactant in humans, diminishes accumulation of neutrophils at inflammatory sites. To evaluate underlying mechanisms, we have studied whether CRP and peptides derived from could affect first step neutrophil extravasation, L-selectin-mediated interaction with endothelial cells. markedly attenuated attachment human to cultured LPS-activated coronary artery pulmonary microvascular cells apparent IC50 values 20 22 microg/ml, respectively. At...
Summary Objective In view of the association hyperprolactinaemia with insulin resistance, we hypothesized that patients may present increased cardiovascular risk markers. Design Descriptive clinical trial. Methods Serum glucose, insulin, lipids, high sensitivity C‐reactive protein (hsCRP), interleukin (IL)‐6, tumour necrosis factor (TNF)‐α and soluble E‐selectin (sELAM‐1) serum levels were determined in 15 at baseline (compared 20 healthy subjects) after 12 weeks cabergoline therapy (0·5–1...
Research Article30 April 2019Open Access Transparent process Control of anterior GRadient 2 (AGR2) dimerization links endoplasmic reticulum proteostasis to inflammation Marion Maurel INSERM U1242, “Chemistry, Oncogenesis Stress Signaling”, University Rennes, France Centre de Lutte Contre le Cancer Eugène Marquis, VIB Department Medical Protein Research, UGent, Gent, Belgium Apoptosis Centre, School Natural Sciences, NUI Galway, Ireland Search for more papers by this author Joanna Obacz Tony...
Ovarian cancer is the most lethal of all gynecological malignancies, due in part to diagnosis at an advanced stage caused by lack specific signs and symptoms absence reliable tests for screening early detection. Most patients will respond initially treatment but about 70% them suffer a recurrence. Therefore, new therapeutic modalities are urgently needed overcome chemoresistance observed ovarian patients. Evidence accumulates suggesting that insulin/insulin growth factor (IGF) pathways could...
Abstract EFTUD2 is mutated in patients with mandibulofacial dysostosis microcephaly (MFDM). We generated a mutant mouse line conditional mutation Eftud2 and used Wnt1-Cre2 to delete it neural crest cells. Homozygous deletion of causes brain craniofacial malformations, affecting the same precursors as MFDM patients. RNAseq analysis embryonic heads revealed significant increase exon skipping increased levels an alternatively spliced Mdm2 transcript lacking 3. Exon was also O9-1 cells after...
Human biological specimens are important for translational research programs such as the Canadian Ovarian Experimental Unified Resource (COEUR) funded by Terry Fox Research Institute. Sample quality is an consideration, it directly impacts of ensuing research. The aim present study was to determine tissues collected from different sites contributing COEUR cohort. Samples high-grade serous ovarian tumors (fresh frozen and corresponding paraffin-embedded tissues) were provided nine...
Purpose Epithelial-mesenchymal transition (EMT) is a critical process for cancer metastasis and recurrence. Metformin, an effective oral antidiabetic drug, has been associated with decreased risk mortality. In this pilot study, we started to evaluate the effect of metformin on EMT in vivo vitro endometrial (EC). Methods Endometrial cell lines freshly isolated EC tumor specimens were used assess after treatment. Cell subjected wound healing AlamarBlue assays determine migration proliferation;...
The transmembrane emp24 domain/p24 (TMED) family are essential components of the vesicular transport machinery. Members TMED serve as cargo receptors implicated in selection and packaging endoplasmic reticulum (ER) luminal proteins into coatomer (COP) II coated vesicles for anterograde to Golgi. Deletion or mutations Tmed genes yeast Drosophila results ER-stress activation unfolded protein response (UPR). UPR leads expression important expanding folding capacity ER, degrading misfolded...
Heterozygous mutations in SNRPB, an essential core component of the five small ribonucleoprotein particles spliceosome, are responsible for cerebrocostomandibular syndrome (CCMS). We show that Snrpb heterozygous mouse embryos arrest shortly after implantation. Additionally, deletion developing brain and neural crest cells models craniofacial malformations found CCMS, results death birth. RNAseq analysis mutant heads prior to morphological defects revealed increased exon skipping intron...
FH is associated with accelerated atherosclerosis. Based on the crucial role of macrophage LPL in atherogenesis, we determined present study expression patients FH. Monocytes isolated from 13 and control subjects were differentiated into macrophages by culturing cells for 9 days 20% autologous or heterologous serum. Macrophages cultured their own sera showed a significant increase mRNA levels, extracellular mass, activity compared subjects. Although these alterations positively correlated...
Impairment of homologous recombination (HR) is found in close to 50 % ovarian and breast cancer. Tumors with BRCA1 mutations show increased expression the Insulin-like growth factor type 1 receptor (IGF-1R). We previously have shown that inhibition IGF-1R results apoptosis tumor cells. In current study, we aimed investigate correlation between HR sensitivity inhibition. Further, hypothesized might sensitize proficient cancers Poly ADP ribose polymerase (PARP) inhibitors. Using cancer...
Mutations in EFTUD2 are responsible for the autosomal dominant syndrome named MFDM (mandibulofacial dysostosis with microcephaly). However, it is not clear how reduced levels of cause abnormalities associated this syndrome. To determine if mouse can serve as a model uncovering etiology found patients, we used situ hybridization to characterize expression Eftud2 during development, and CRISPR/Cas9 generate mutant line deletion exon 2 gene. We that was expressed throughout embryonic though its...
PARP inhibitors have shown promising clinical results in cancer patients carrying BRCA1/2 mutations. Their efficacy could logically be influenced by PARP1 protein levels patient tumors. We screened three cohorts of with ovarian cancer, totaling 313 samples, and evaluated expression immunohistochemistry further validation western blotting. observed that up to 60 % tumors showed little expression. In serous tumors, comparing intratumoral between chemo-naïve post-chemotherapy revealed a...
Hyperhomocysteinemia is an independent risk factor for cardiovascular disease in human diabetes. Among the multiple factors that may account atherogenicity of homocysteine (Hcys) patients with diabetes, macrophage (Mo) lipoprotein lipase (LPL) has unique features it increased diabetes and acts as a proatherogenic arterial wall. In present study, we determined direct regulatory effect Hcys on Mo LPL gene expression secretion. Incubation J774 increased, time- dose-dependent manner, mRNA...