A5021679875- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Toxin Mechanisms and Immunotoxins
- Respiratory viral infections research
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- RNA regulation and disease
Kansas State University
2025
University of Pittsburgh
2020-2024
Children's Hospital of Pittsburgh
2020-2024
Abstract Mammalian orthoreovirus (reovirus) infects most mammals and is associated with celiac disease in humans. In mice, reovirus the intestine disseminates systemically to cause serotype-specific patterns of brain. To identify receptors conferring serotype-dependent neuropathogenesis, we conducted a genome-wide CRISPRa screen identified paired immunoglobulin-like receptor B (PirB) as candidate. Ectopic expression PirB allowed binding infection. extracelluar D3D4 region required for...
ABSTRACT Synthesis of subgenomic RNAs is a strategy commonly used by polycistronic positive-sense single-stranded RNA viruses to express 3′-proximal genes. Members the order Nidovirales , including coronaviruses and arteriviruses, use unique discontinuous transcription synthesize RNAs. In this study, in silico synonymous site conservation analysis structure folding predicted existence intra-family conserved high-order within M ORF arteriviral genomes, which was further confirmed secondary...
ABSTRACT Pathological effects of apoptosis associated with viral infections the central nervous system are an important cause morbidity and mortality. Reovirus is a neurotropic virus that causes in neurons, leading to lethal encephalitis newborn mice. Reovirus-induced diminished mice germline ablation NF-κB subunit p50. It not known whether pro-apoptotic function mediated by neuron-intrinsic processes, NF-κB-regulated cytokine production inflammatory cells, or combination both. To determine...
Pathological effects of apoptosis associated with viral infections the central nervous system are an important cause morbidity and mortality. Reovirus is a neurotropic virus that causes in neurons, leading to lethal encephalitis newborn mice. Reovirus-induced diminished mice germ line ablation NF-κB subunit p50. It not known whether proapoptotic function mediated by neural-cell-intrinsic (neural-intrinsic) processes, NF-κB-regulated cytokine production inflammatory cells, or combination...