A5103060017- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Celiac Disease Research and Management
- Diabetes and associated disorders
- Diabetes Management and Research
- Pancreatic function and diabetes
- RNA regulation and disease
- Immune Response and Inflammation
- Genetics and Neurodevelopmental Disorders
Vanderbilt University Medical Center
2016-2022
Vanderbilt University
2018
University of Pittsburgh
2018
A nonpathogenic virus can promote inflammatory immunity to dietary antigens and may be linked the development of celiac disease.
ABSTRACT Lambda interferon (IFN-λ) has potent antiviral effects against multiple enteric viral pathogens, including norovirus and rotavirus, in both preventing curing infection. Because the intestine includes a diverse array of cell types, however, cell(s) upon which IFN-λ acts to exert its is unclear. Here, we sought identify IFN-λ-responsive cells by generation mice with lineage-specific deletion receptor for IFN-λ, Ifnlr1 . We found that expression IFNLR1 on intestinal epithelial (IECs)...
ABSTRACT Several viruses induce intestinal epithelial cell death during enteric infection. However, it is unclear whether proapoptotic capacity promotes or inhibits replication in this tissue. We infected mice with two reovirus strains that infect the intestine but differ to alter immunological tolerance new food antigen. Infection strain T1L, which induces an inflammatory immune response fed antigen, prolonged intestine, whereas T3D-RV, does not response, rapidly cleared from intestine....
Numerous compounds stimulate rodent β-cell proliferation; however, translating these findings to human β-cells remains a challenge. To examine proliferation in response such compounds, we developed medium-throughput vitro method of quantifying adult markers. This is based on high-content imaging dispersed islet cells seeded 384-well plates and automated cell counting that identifies fluorescently labeled with high specificity using both nuclear cytoplasmic β-Cells from each donor were...
ABSTRACT Pathological effects of apoptosis associated with viral infections the central nervous system are an important cause morbidity and mortality. Reovirus is a neurotropic virus that causes in neurons, leading to lethal encephalitis newborn mice. Reovirus-induced diminished mice germline ablation NF-κB subunit p50. It not known whether pro-apoptotic function mediated by neuron-intrinsic processes, NF-κB-regulated cytokine production inflammatory cells, or combination both. To determine...
Pathological effects of apoptosis associated with viral infections the central nervous system are an important cause morbidity and mortality. Reovirus is a neurotropic virus that causes in neurons, leading to lethal encephalitis newborn mice. Reovirus-induced diminished mice germ line ablation NF-κB subunit p50. It not known whether proapoptotic function mediated by neural-cell-intrinsic (neural-intrinsic) processes, NF-κB-regulated cytokine production inflammatory cells, or combination...