A5032676285- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiovascular Function and Risk Factors
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Peptidase Inhibition and Analysis
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neural Engineering
- Thyroid Disorders and Treatments
- Adipose Tissue and Metabolism
- Cardiac pacing and defibrillation studies
- Hormonal Regulation and Hypertension
- Cardiac Ischemia and Reperfusion
- Diabetes and associated disorders
- Congenital heart defects research
- Cardiac Arrhythmias and Treatments
- Caveolin-1 and cellular processes
- Mitochondrial Function and Pathology
- Neurological disorders and treatments
- Stress Responses and Cortisol
- Chemical Synthesis and Analysis
- Nitric Oxide and Endothelin Effects
- Zebrafish Biomedical Research Applications
- Pain Mechanisms and Treatments
University of the Basque Country
2013-2024
Universidad Francisco de Vitoria
2014
University of Utah
1998-2007
Masonic Medical Research Laboratory
2007
University of Cologne
2007
Hôpital Cardiologique du Haut-Lévêque
2007
Ophthalmic Consultants of Long Island
2007
Heidelberg University
2007
University Hospital Heidelberg
2007
Czech Academy of Sciences
2003
Cardiac ion channelopathies are responsible for an ever-increasing number and diversity of familial cardiac arrhythmia syndromes. We describe a new clinical entity that consists ST-segment elevation in the right precordial ECG leads, shorter-than-normal QT interval, history sudden death.Eighty-two consecutive probands with Brugada syndrome were screened channel gene mutations direct sequencing. Site-directed mutagenesis was performed, CHO-K1 cells cotransfected cDNAs encoding wild-type or...
Abstract Diabetes mellitus (DM) encompasses a multitude of secondary disorders, including heart disease. One the most frequent and potentially life threatening disorders DM-induced disease is ventricular tachycardia (VT). Here we show that toll-like receptor 2 (TLR2) NLRP3 inflammasome activation in cardiac macrophages mediate production IL-1β DM mice. causes prolongation action potential duration, induces decrease potassium current an increase calcium sparks cardiomyocytes, which are...
1,3-Bis-(2-hydroxy-5-trifluoromethyl-phenyl)-urea (NS1643) is a newly discovered activator of human <i>ether-a-go-go</i>-related gene (hERG) K<sup>+</sup> channels. Here, we characterize the effects this compound on cloned hERG channels heterologously expressed in <i>Xenopus laevis</i> oocytes. When assessed with 2-s depolarizations, NS1643 enhanced magnitude wild-type current concentration- and voltage-dependent manner an EC<sub>50</sub> 10.4 μM at –10 mV. The fully activated...
Voltage-gated ion channels are essential for membrane potential maintenance, homeostasis, electrical signal production and controlling the Ca 2+ flow through membrane. Among all channels, key regulators of neuronal excitability voltage-gated potassium (K V ), largest family K + channels. Due to ROS high levels in aging brain, might be affected by oxidative agents neurodegeneration processes. This review provides new insight about channelopathies most studied neurodegenerative disorders, such...
A great variety of alterations have been described in the nervous system diabetic animals. They are named as neuropathy and affect brain, spinal cord peripheral nerves. In animals, plasma tissue catecholamine levels reported to be increased, decreased or unchanged, these disparities explained by differences tissues selected, severity duration diabetes. Dopamine, norepinephrine epinephrine from different were extracted absorption onto alumina, measured high performance liquid chromatography...
Abstract The effects of propafenone on the delayed rectifier K + current were studied in guinea pig ventricular myocytes by using patch-clamp technique. In these myocytes, this consists at least two components: a La 3+ -sensitive component activating rapidly with moderate depolarizations and -resistant slowly more positive potentials. absence (when both components are present), inhibited outward current, its being marked after weak than strong depolarizations. Propafenone-induced block tail...
In this work we have combined biochemical and electrophysiological approaches to explore the modulation of rat ventricular transient outward K(+) current (I(to)) by calmodulin kinase II (CaMKII). Intracellular application CaMKII inhibitors KN93, calmidazolium, autocamtide-2-related inhibitory peptide (ARIP-II) accelerated inactivation I(to), even at low [Ca(2+)]. same conditions, coimmunoprecipitated with Kv4.3 channels, suggesting that phosphorylation channels modulate I(to). Because...
alpha(1)-Adrenoceptor stimulation prolongs the duration of cardiac action potentials and leads to positive inotropic effects by inhibiting transient outward K(+) current (I(to)). In present study, we have examined role several protein kinases G involved in I(to) inhibition response alpha(1)-adrenoceptor isolated adult rat ventricular myocytes. Our findings exclude classic alpha(1)-adrenergic pathway: activation G(alphaq), phospholipase C (PLC), kinase (PKC), because neither PLC, nor PKC,...
The fast transient outward current, I(to,fast) , is the most extensively studied cardiac K(+) current in diabetic animals. Two hypotheses have been proposed to explain how type-1 diabetes reduces this muscle. first one a deficiency channel expression due defect trophic effect of insulin. second proposes flawed glucose metabolism as cause reduced . Moreover, little information exists about effects and possible mechanisms on other repolarizing currents human heart: I(to,slow) I(Kr) I(Ks)...
M-channels are voltage-gated potassium channels composed of Kv7.2-7.5 subunits that serve as important regulators neuronal excitability. Calmodulin binding is required for Kv7 channel function and mutations in Kv7.2 disrupt calmodulin cause Benign Familial Neonatal Convulsions (BFNC), a dominantly inherited human epilepsy. On the basis mutants deficient not functional, has been defined an auxiliary subunit channels. However, we have identified presumably phosphomimetic mutation S511D permits...
The electrocardiogram of hypothyroid patients shows a series abnormalities cardiac repolarization due to reduction some repolarizing K(+) currents and an increase the L-type calcium current. Experimental clinical works call into question unique role T3 T4 in these mechanisms correlate increased serum TSH levels with both subclinical overt hypothyroidism. In this context, aim present study was investigate direct effects upon electrical properties.The action potential recording ion channel...
Hypothyroidism, the most common endocrine disease, induces cardiac electrical remodeling that creates a substrate for ventricular arrhythmias. Recent studies report high thyrotropin (TSH) levels are related to abnormalities and increased mortality rates. The aim of present work was investigate direct effects TSH on heart its possible causative role in incidence arrhythmia hypothyroidism.
Imipramine, amitriptyline, mianserine, maprotiline, and trazodone are five widely used antidepressant drugs with different chemical structures. Imipramine amitriptyline tricyclics, mianserine maprotiline tetracyclics, is a triazolopyridine derivative. We studied the effects of these on transient outward K+ current (Ito) interaction mechanisms within drug molecules channel-binding site. The mainly responsible for action-potential repolarization in rat ventricle, all block Ito, but manners....
KCNE1 encodes an auxiliary subunit of cardiac potassium channels. Loss-of-function variations in this gene have been associated with the LQT5 form long QT syndrome (LQTS), secondary to reduction IKs current. We present a case which D85N rare polymorphism is LQT2 phenotype. An 11-year old competitive athlete presented mild bradycardia and QTc interval 470 ms. phenotype, consisting low-voltage bifid T waves, was evident right precordial electrocardiogram leads. During tachycardia phase...