A5044439645- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Reproductive Biology and Fertility
- Renal and related cancers
- Glioma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Histone Deacetylase Inhibitors Research
- CRISPR and Genetic Engineering
- Signaling Pathways in Disease
- DNA and Nucleic Acid Chemistry
- Prostate Cancer Treatment and Research
- Estrogen and related hormone effects
- Prenatal Screening and Diagnostics
- Drug Transport and Resistance Mechanisms
- Retinal Development and Disorders
- Tissue Engineering and Regenerative Medicine
- Click Chemistry and Applications
- Gene expression and cancer classification
- Radiopharmaceutical Chemistry and Applications
- Renal cell carcinoma treatment
- Sexual Differentiation and Disorders
West Virginia University
2025
University of Central Lancashire
2014-2021
University of Manchester
2009-2019
Manchester Academic Health Science Centre
2019
Canisius College
2014-2015
Royal Preston Hospital
2014
St Mary's Hospital
2013
St. Mary’s Hospital
2010
Durham University
2009
Blue cone monochromacy (BCM) is an X-linked retinal disorder caused by mutations in the OPN1LW/OPN1MW gene locus, resulting impaired function and structural degeneration. We conducted a comparative analysis of AAV-mediated therapy Opn1lw/Opn1mw double knockout (DKO) Opn1mw C198R /Opn1sw -/- (C198R) BCM mouse models evaluated therapeutic window, efficacy, longevity. Our results demonstrate that AAV8-Y733F capsid achieved superior rescue compared to AAV5. While both DKO showed similar windows...
Identification and characterisation of differentially regulated genes in preimplantation human embryonic development are required to improve embryo quality pregnancy rates IVF. In this study, we examined expression a number known be critical for early compared profiles individual embryos establish any differences gene fresh frozen-thawed used routinely We analysed 19 by cDNA amplification followed quantitative real-time PCR panel 44 embryos. Fresh were obtained from surplus cleavage stage...
Early development in humans is characterised by low and variable embryonic viability, reflected fecundity high rates of miscarriage, relative to other mammals. Data from assisted reproduction programmes provides additional evidence that this largely mediated at the level competence highly heterogeneous among embryos. Understanding basis heterogeneity has important implications a number areas including: regulation early human development, disorders pregnancy, programmes, long term health...
Little is understood of the molecular mechanisms involved in earliest cell fate decision human development, leading to establishment trophectoderm (TE) and inner mass (ICM) stem population. Notably, there a lack understanding how transcriptional networks arise during reorganisation embryonic genome post-fertilisation. We identified hierarchical structure preimplantation gene network modules around time activation (EGA). Using models along with eukaryotic initiation factor (EIF)...
Malignant glioma is characterised by a rapid growth rate and high capacity for invasive infiltration to surrounding brain tissue; hence, diagnosis treatment difficult patient survival poor. Aptamers contribute promising unique technology the in vitro imaging of live cells tissues, with potentially bright future clinical diagnostics therapeutics malignant glioma. The binding selectivity, uptake target two DNA aptamers, SA43 SA44, were investigated tissues. assay showed that SA44 bound strong...
Exposure to estrogenic compounds has been shown epigenetically reprogram the prostate and may contribute cancer. The goal of this study was determine effect physiological doses estradiol bisphenol A (BPA) on expression histone modifying enzymes (HMEs) in Using two human cancer cell lines we examined Set8, a methyltransferase, Sirt1, deacetylase, after exposure estrogen or BPA. These experiments were carried out presence natural hormones understand impact additional BPA HME expression. We...
Abstract Human embryonic stem cells (hESCs) derived from the pluripotent Inner cell mass (ICM) of blastocyst are fundamental tools for understanding human development, yet not identical to their tissue origin. To investigate this divergence we compared transcriptomes genetically paired ICM and trophectoderm (TE) samples with three hESC lines: MAN1, HUES3 HUES7 at similar passage. We generated inferred interactome networks using transcriptomic data unique or TE, defined a hierarchy modules...
INTRODUCTION: Many in vitro blood brain barrier (BBB) models have been designed to study the underlying mechanism involved drug transport across BBB, but most not included glioma or cells of human origin. Trans-endothelial electrical resistance (TEER) is a key measure membrane potential and can be used quantify tightness BBB. The aim this was evaluate integrity various BBB as determined by TEER measurements. METHOD: Varying combinations co-culture were set up using primary origin which...
Prostate cancer is the most common form of for males and it has been shown that exposure to estrogenic compounds can increase likelihood developing prostate cancer. The goal this study determine effect physiological doses two compounds, estradiol (E2) bisphenol A (BPA), on expression histone modifying enzymes (HMEs) in cells. Alterations HMEs could potentially lead global changes gene may play an important role development due increased E2 or BPA. To these cancer, cell lines, LNCaP PC3, were...
INTRODUCTION: Substituted indoles and related structures have been shown to exhibit potent anticancer activity against breast cancer cell lines. Here, the effects of structurally similar substituted human glial lines, 1321N1 U87MG, investigated by comparing these compounds conventional anti drugs. METHODS: Cell viability in presence test was measured using an MTS assay corroborated ATP proliferation as well a Trypan blue exclusion test. The significance reactive oxygen species (ROS) process...
INTRODUCTION: Glioma represent less than 2% of all cancer cases in the UK, however, 80% these tumours are glioblastoma (GBM). GBM is a highly aggressive tumour with poor patient survival rates, despite treatment involving surgery and radiotherapy adjuvant chemotherapy. Delivery systems that have ability to distinguish neoplasms from non-cancerous tissues, such as aptamers, may improve outcome. Aptamers previously been shown selectively target glioma cell lines. can also be raised known...
The development of biomarker panels for glioblastoma has the potential to greatly improve outcome treatment by assisting in earlier detection, determining response and predicting prognosis therapeutic strategy. MicroRNAs (miRNAs) present circulation patients provide a relatively non-invasive source markers this purpose. Gliomas, including glioblastoma, release miRNAs exosomes protein complexes which alter expression levels serum patients. aim study was isolate identify deregulated from sera...
MicroRNAs are short non-coding RNAs that act as micro-managers of cellular function. Their dysregulation is well documented in many cancers. TCGA microRNA expression data available to identify the prognostic potential microRNAs malignant glioma. Using LASSO statistical approach for 475 glioblastomas dataset we identified a nine-microRNA signature predicts overall (p = 2.26e-09) and progression-free survival 9.91e-08) with superior power than conventional molecular markers, including MGMT...
INTRODUCTION: Gliomas, including glioblastoma, release both microvesicles containing microRNA (miRNA) and miRNA-protein complexes which can be isolated from biological fluids such as serum. Circulating miRNA used biomarkers for earlier diagnosis, predicting response to treatment prognostic information. The aim of this study was identify a panel circulating glioma. METHOD: MiRNAs were the sera GBM (n = 26) control patients 23), using phenol-chloroform extraction. relative expression 84 miRNAs...
INTRODUCTION: Aptamers are in vitro generated DNA and RNA sequences which randomly created as a library, with multiple permutations combinations. These then exposed to the target structure against we want an aptamer ‘selected’ using Sequential Enumeration of Ligands by Exponential enrichment (SELEX). METHOD: Commercially available glioma glial cell lines in-house primary cultures were used. Modified aptamers based on published newly used project identify their binding targets. Cy3 or biotin-...
Aptamers are in vitro generated DNA and RNA sequences. These randomly present a library with common primers. Aptamer sequences have multiple permutations combinations of nucleotides then ‘selected’, using Sequential Enumeration Ligands by Exponential Enrichment (SELEX) towards the desired target, known or unknown. Modified aptamers, based on published against glioma cell lines novel aptamers were used project to identify confirm targets. Commercially available short term cultures from...
INTRODUCTION: Targeted drug delivery via aptamers may reduce non-specific toxicity of chemotherapy by selectively directing anti-cancer drugs to tumour cells. The study aimed examine the binding selectivity DNA on glial cell lines and primary glioma tissues. METHOD: Aptamers SA44DNA SA43DNA tagged with Cy3 fluorescent dye were screened analysed through confocal microscopy flow cytometry. Primary tissues from patients: grade I(n = 7), II(n 14), III(n 10), IV(n 10) non-cancerous brain(n 12)...