A5031083183- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Luminescence and Fluorescent Materials
- Photoreceptor and optogenetics research
- Hematopoietic Stem Cell Transplantation
- Plant Virus Research Studies
- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Venomous Animal Envenomation and Studies
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Analytical Chemistry and Sensors
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Cellular transport and secretion
- Mesenchymal stem cell research
- DNA and Nucleic Acid Chemistry
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- Neuroscience and Neural Engineering
- Electrochemical sensors and biosensors
Institute of Molecular Biology
2014-2023
ETH Zurich
2009
École Polytechnique Fédérale de Lausanne
2005
Semliki forest virus (SFV) is internalized by clathrin-mediated endocytosis, and transported via early endosomes to late lysosomes. The intracellular pathway taken individual fluorescently labeled SFV particles was followed using immunofluorescence in untransfected cells, video-enhanced, triple-color fluorescence microscopy live cells transfected with GFP- RFP-tagged Rab5, Rab7, Rab4, Arf1. viruses progressed from Rab5-positive a population of (about 10% total) that contained both Rab5 Rab7....
Biocompatible organic dyes emitting in the near-infrared are highly desirable fluorescence imaging techniques. Herein we report a synthetic approach for building novel small peri-guanidine-fused naphthalene monoimide and perylene chromophores. The presented structures possess absorption emission, high photostability, good water solubility. After fast cellular uptake, they selectively stain mitochondria with low background live fixed cells. They can be additionally modified one-step reaction...
Abstract Enhancers are important cis-regulatory elements controlling cell-type specific expression patterns of genes. Furthermore, combinations enhancers and minimal promoters utilized to construct small, artificial for gene delivery vectors. Large-scale functional screening methodology genomic maps enhancer activities has been successfully established in cultured cell lines, however, not yet applied terminally differentiated cells tissues a living animal. Here, we transposed the...
Large-scale RNAi screening has become an important technology for identifying genes involved in biological processes of interest. However, the quality large-scale is often deteriorated by off-targets effects. In order to find statistically significant effector pathogen entry, we systematically analyzed entry pathways human host cells eight pathogens using image-based kinome-wide siRNA screens with siRNAs from three vendors. We propose a Parallel Mixed Model (PMM) approach that simultaneously...
// Jeannette Boutter 1, 8, * , Yun Huang Blerim Marovca 8 Andreas Vonderheit 2 Michael A. Grotzer Cornelia Eckert 3 Gunnar Cario 4 Bernd Wollscheid 5 Peter Horvath 6, 7 Beat C. Bornhauser Jean-Pierre Bourquin 1 Department of Pediatric Oncology, University Children's Hospital Zurich, Switzerland Institute Molecular Biology, Mainz, Germany Oncology/Hematology, Charité Universitätsmedizin Berlin, Pediatrics, Medical Centre Schleswig-Holstein, Kiel, Systems ETH 6 Synthetic and Biology Unit,...
Enhancers are important cis-regulatory elements controlling cell-type specific expression patterns of genes. Furthermore, combinations enhancers and minimal promoters utilized to construct small, artificial promotors for gene delivery vectors. Large-scale functional screening methodology genomic maps enhancer activities has been successfully established in cultured cell lines, however, not yet applied terminally differentiated cells tissues a living animal. Here, we transposed the...