A5031425470- Asthma and respiratory diseases
- Monoclonal and Polyclonal Antibodies Research
- Mast cells and histamine
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Eosinophilic Disorders and Syndromes
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Immunodeficiency and Autoimmune Disorders
- Inflammatory Myopathies and Dermatomyositis
- Viral Infections and Immunology Research
- Urticaria and Related Conditions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Diabetes and associated disorders
- Renal Diseases and Glomerulopathies
- Systemic Sclerosis and Related Diseases
- Cell Adhesion Molecules Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Systemic Lupus Erythematosus Research
- Chronic Lymphocytic Leukemia Research
- S100 Proteins and Annexins
- Allergic Rhinitis and Sensitization
- NF-κB Signaling Pathways
- Dermatological and COVID-19 studies
Kyoto Katsura Hospital
2014
The University of Tokyo
1999-2009
Kumamoto University
2003
University of Tokyo Hospital
2003
Kyushu University
1997-1999
Radboud University Nijmegen
1994-1995
Tokyo Women's Medical University
1994
St. Marianna University School of Medicine
1994
Abstract Normal T cell repertoire contains regulatory cells that control autoimmune responses in the periphery. One recent study demonstrated CD4+CD25+ were generated from autoreactive without negative selection. However, it is unclear whether, general, positive selection and of are mutually exclusive processes thymus. To investigate ontogeny cells, neo-autoantigen-bearing transgenic mice expressing chicken egg OVA systemically nuclei (Ld-nOVA) crossed with an OVA-specific TCR (DO11.10)....
To determine what kinds of CSF modulate human basophil function, recombinant or purified hemopoietic growth factors were tested for the effect on histamine release from basophils. Both granulocyte (G)-macrophage (M) and IL-3 markedly enhanced upon stimulation with anti-IgE in a dose-dependent manner (maximal enhancement 25.5% by GM-CSF 30.8% as expressed percent increase against total cellular content), whereas G-CSF, M-CSF, IL-4 had no effect. Enhancing action these was still observed...
Peroxisome proliferator-activated receptor-gamma (PPAR gamma) controls adipogenesis and glucose metabolism. It was reported recently that PPAR gamma activation by its agonistic ligands modifies lymphocyte function. Since synthetic are known to exert their effect via gamma-dependent -independent pathways, we examined the physiological role of in lymphocytes using heterozygote mutant mice which one allele is deleted gamma(+/-)). In contrast T cells, did not exhibit a significant difference, B...
Substantial evidence indicates a close relationship between eosinophils and basophils. We examined whether interleukin 5 (IL-5), known to be eosinophilopoietic capable of selectively regulating eosinophil functions, has an affect on basophil functions. IL-5 enhanced histamine release evoked by anti-IgE, formyl-methionyl-leucyl-phenylalanine, or ionophore A23187 at picomolar concentrations. Direct action human basophils was confirmed using highly purified populations. These observations...
Abstract Transfer of the αβ TCR genes into T lymphocytes will provide a means to enhance Ag-specific immunity by increasing frequency tumor- or pathogen-specific lymphocytes. We generated an efficient gene transfer system using two independent monocistronic retrovirus vectors harboring either class II MHC-restricted α β specific for chicken OVA. The enabled us express clonotypic in 44% CD4+ cells. transduced cells showed remarkable response OVA323–339 peptide vitro culture system, and Ag was...
Objective. To evaluate the clinical usefulness of measuring anti-RNA polymerase (RNAP) III antibody with a commercially available ELISA in Japanese patients SSc. Methods. This multicentre study involved 354 SSc, 245 non-SSc CTDs and 102 healthy controls. ELISAs were used to detect anti-RNAP antibody, anti-topo I ACA. The presence selected serum samples was confirmed by immunoprecipitation (IP) assay. Results. By ELISA, detected 38 (10.7%) 3 (1.2%) CTD no specificity for SSc excellent...
Abstract Several recent studies have identified eosinophils as a cellular source of various cytokines, indicating that play not only an effector role, but also regulatory role within the allergic inflammatory cell network. In this study, we demonstrate can generate and secrete monocyte chemoattractant protein‐1 (MCP‐1), prototype C‐C chemokines. Eosinophils generated immunoreactive MCP‐1 in response to such diverse stimuli C5a, formylmethionyl‐leucyl‐phenylalanine (FMLP) ionomycin,...
Abstract For the treatment of rheumatoid arthritis, efficient drug delivery methods to inflamed joints need be developed. Because T cells expressing an appropriate autoantigen-specific receptor can migrate lesions, it has been reasoned that they employed deliver therapeutic agents. To examine ability and efficiency such as a vehicle, we experimentally induced model arthritis. Splenic from DO11.10 TCR transgenic mice specific for OVA were transduced with murine IL-10. Adoptive transfer...
We have previously reported that C57B1/6 mice develop lung lesions similar to human hypersensitivity pneumonitis (HP) by repeated transnasal administration of Thermoactinomyces vulgaris antigen. Since the HP-like were induced via respiratory route and causative antigen in HP (farmer's lung), it seems this murine model is useful for investigating cell-to-cell interactions HP. To clarify involvement mast cells (MC) development HP, T. (90 micrograms/day) was transnasally administered...
Abstract Gene transfer of TCR αβ-chains into T cells may be a promising strategy for providing valuable lymphocytes in the treatment tumors and other immune-mediated disorders. We report this study reconstitution CD8+ by αβ-chain genes derived from an infiltrating cell P815. Analysis clonal expansion Vβ subfamily usage TIL tumor sites demonstrated that using Vβ10 efficiently infiltrated expanded clonally. The α- β-chain sequences tumor-infiltrating CD8+/Vβ10+ single clone (P09-2C clone) were...
Anti-56K autoantibodies are present in sera from patients with various autoimmune diseases, predominantly rheumatoid arthritis, systemic lupus erythematosus, or Sjogren's syndrome. To clarify the molecular structure of this autoantigen, we isolated a 2.0-kilobase pair cDNA clone considered to encode full-length 56K autoantigen. The longest open reading frame encodes 505-amino acid polypeptide, predicted mass 54.4 kDa. vitro translated protein is recognized by all anti-56K positive patient...
Abstract The mechanism of autoantibody production in autoimmune diseases is not well understood. In the present study we performed B cell epitope mapping U1 small nuclear ribonucleoprotein (snRNP)‐C, one target molecules anti‐nRNP to investigate how cells respond autoantigen. After cloning and expression a full length complementary DNA (cDNA) encoding U1‐C protein, several truncated mutants cDNA were constructed expressed E. coli . Although few epitopes distributed on whole molecule, all...
<i>Background:</i> Accumulating evidence indicates that eotaxin is the primary mediator of tissue eosinophilia. In present study, we analyzed mechanisms generation by Th1-/Th2-derived cytokines in vitro. <i>Methods:</i> Human dermal fibroblasts, human umbilical vein endothelial cells and A549 bronchial epithelial cell line were stimulated with TNF-α, IL-4, IFN-γ or TNF-α combination IL-4 amount production was analyzed. <i>Results:</i> Fibroblasts produced...
Abstract Objective Chondromodulin I (ChM‐I), a cartilage matrix protein, promotes the growth and proteoglycan synthesis of chondrocytes. However, it also inhibits angiogenesis. Since ChM‐I is expressed not only in cartilage, but thymus, we investigated modulation T cell function by to assess its therapeutic potential rheumatoid arthritis (RA). Methods The localization expression mouse thymus tissue was examined situ hybridization. proliferative response peripheral blood cells synovial...
Wegener's granulomatosis with eosinophilia both in blood and tissue samples has been rarely reported. A 25-year-old woman developed fever, granulomatous inflammation of nasal mucosa, pulmonary hemorrhage, suprascleritis, cutaneous vasculitis. She showed biospy skin, lung. was positive for proteinase 3-antineutrophil cytoplasmic antibody. had no history asthma. We diagnosed her as rather than Churg-Strauss syndrome. Her mucocutaneous lesions were successfully treated high-dose corticosteroid...
One of the hallmarks systemic autoimmune diseases is immune responses to nuclear autoantigens. We have examined fate response against a autoantigen using human U1 small ribonucleoprotein-A protein (HuA) transgenic (Tg) mice by adoptive transfer autoreactive lymphocytes. obtained two Tg lines that different expression levels transgene. After spleen cells from HuA-immunized wild-type were transferred and their non-Tg littermates, these recipients injected with HuA/IFA induce recall memory...