A5067472214- Textile materials and evaluations
- Color perception and design
- Industrial Vision Systems and Defect Detection
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Neonatal and fetal brain pathology
- Neuroscience of respiration and sleep
- Nerve injury and regeneration
- Color Science and Applications
- Hydrogen's biological and therapeutic effects
- Prenatal Substance Exposure Effects
- Stress Responses and Cortisol
- Birth, Development, and Health
- Alzheimer's disease research and treatments
- Pancreatic function and diabetes
- Manufacturing Process and Optimization
- Neuroendocrine regulation and behavior
- Effects and risks of endocrine disrupting chemicals
- Cannabis and Cannabinoid Research
- Child Abuse and Trauma
- Supramolecular Self-Assembly in Materials
- Pluripotent Stem Cells Research
- Infrared Thermography in Medicine
- Cellular transport and secretion
- Neurotransmitter Receptor Influence on Behavior
Kagawa University
2014-2025
Tokushima University
1979-2012
Taiyo Yuden (Japan)
2005-2011
Osaka Prefecture University
2011
Tokyo Institute of Technology
1975-2009
Mukogawa Women's University
2003-2009
Tottori University
2005-2009
National Cerebral and Cardiovascular Center
2005
Jichi Medical University Hospital
2002-2003
Jichi Medical University
2002-2003
To determine what kinds of CSF modulate human basophil function, recombinant or purified hemopoietic growth factors were tested for the effect on histamine release from basophils. Both granulocyte (G)-macrophage (M) and IL-3 markedly enhanced upon stimulation with anti-IgE in a dose-dependent manner (maximal enhancement 25.5% by GM-CSF 30.8% as expressed percent increase against total cellular content), whereas G-CSF, M-CSF, IL-4 had no effect. Enhancing action these was still observed...
An alkalophilic bacterium belonging to the genus Bacillus was isolated from an indigo ball. The exhibited a maximum growth rate at pH 10-0 TO 10-5. incorporation of 14C-labelled amino acids or [14C]uracil, uptake alpha-amino isobutyric acid into and oxygen consumption with as substrates were all 9-0 [U-14C]glucose organism carbohydrates, on other hand, showed little variation in 8 10 region. intact bacteria protoplasts culture medium 10. membrane oxidized NADH maximally 7-5, ATPase bound...
Early life stress interrupts brain development through the disturbance of various neurotransmitter and neurotrophic factor activities, but details remain unclear. In current study, we focused on serotonergic system, which plays a critical role in development, examined time-dependent influence prolonged maternal separation male Sprague-Dawley rats. The rats were separated from their dams for 3h twice-daily during postnatal days (PDs) 2-20. was analyzed PDs 7, 14, 21, 28 using HPLC to assess...
Maternal separation (MS) is known to affect hippocampal function such as learning and memory, yet the molecular mechanism remains unknown. We hypothesized that these impairments are attributed abnormities of neural circuit formation by MS, focused on brain-derived neurotrophic factor (BDNF) key because BDNF signaling has an essential role in synapse during early brain development. Using rat offspring exposed MS for 6 h/day postnatal days (PD) 2-20, we estimated hippocampus Our results show...
DNA methyltransferases (DNMTs) are associated with epigenetic regulation of gene expression, and methyl-CpG binding protein 2 (MECP2) acts as a long-range regulator methylated genes. We evaluated the effects bisphenol A (BPA) on embryonic mouse hypothalamic cells, particular emphasis expression Dnmts (Dnmt1, Dnmt3a, Dnmt3b) Mecp2 isoforms. In dose-dependent (0.02-200 µM BPA) 3-hr experiment, real-time reverse transcription polymerase chain reaction revealed that both Mecp2_e2 was affected at...
Early child maltreatment, such as abuse and neglect, is well known to affect the development of social skills. However, mechanisms by which an adverse environment interrupts skills remain unelucidated. Identifying period brain regions that are susceptible environments can lead appropriate developmental care later in life. We recently reported excitatory/inhibitory imbalance low activity during behavior medial prefrontal cortex (mPFC) maternal separation (MS) animal model early life neglect...
We previously reported the neuroprotective potential of combined hydrogen (H
Abstract Despite its poor outcomes, therapeutic hypothermia (TH) is the current standard treatment for neonatal hypoxic-ischaemic encephalopathy (HIE). In this study, due to antioxidant, anti-inflammatory, and antiapoptotic properties, effectiveness of molecular hydrogen (H 2 ) combined with TH was evaluated by means neurological histological assessments. Piglets were divided into three groups: insult normothermia (NT), (TH, 33.5 ± 0.5 °C), H ventilation (TH-H , 2.1–2.7%). administered 24 h....
The phospholipase A and acyltransferase (PLAAT) family is composed of three isoforms in mice (PLAAT1, 3, 5), all which function as phospholipid-metabolizing enzymes exhibiting A1 /A2 activities. Plaat3-deficient (Plaat3-/- ) were previously reported to show lean phenotype remarkable hepatic fat accumulation under high-fat diet (HFD) feeding, while Plaat1-/- have not been analyzed. In the present study, we generated investigated effects PLAAT1 deficiency on HFD-induced obesity, lipid...
Genetic polydactyly/arhinencephaly mouse embryo, Pdn/Pdn, exhibits suppression of Gli3 gene expression. Ochratoxin A (OTA) is a teratogen that causes neural tube defects (NTD) in mice. We investigated gender-dependent differences the incidence NTD induced by OTA Pdn/Pdn mouse. After administering 2 mg/kg to Pdn/+ female mice, mated with males, on day 7.5 gestation, we examined genotypes, sex and fetuses 18. Non-treated had 15.8% risk NTD, all were female. When embryos exposed OTA, increased...
Embedding middle-scale artificial gene networks in live mammalian cells is one of the most important future goals for cell engineering. However, applications highly orthogonal and conventional transcription factors currently available are limited. In this study, we present a scalable pipeline to produce based on homing endonucleases, also known as meganucleases. The introduction mutations at critical sites nuclease activity renders these endonucleases simple but specific DNA binding domain...
Abstract Epithelial-mesenchymal transition (EMT) in primary tumor cells is a key prerequisite for metastasis initiation. Statins, cholesterol-lowering drugs, can delay formation vivo and attenuate the growth proliferation of vitro. The latter effect stronger with mesenchymal-like phenotype than those an epithelial one. However, statins on cancer treated EMT-inducing factors such as transforming factor-β (TGF-β) remains unclear. Here, we examined atorvastatin two cell lines following TGF-β...
Abstract Aim The striatum, a main component of the basal ganglia, is critical part motor and reward systems brain. It consists GABAergic cholinergic neurons receives projections dopaminergic, glutamatergic, serotonergic from other brain regions. Brain‐derived neurotrophic factor (BDNF) plays multiple roles in central nervous system, striatal BDNF has been suggested to be involved psychiatric neurodegenerative disorders. However, transcriptomic impact on striatum remains largely unknown. In...
Against a green background. We have embedded the amyloid β-peptide (Aβ) sequence into fluorescent protein (GFP) structure to generate pseudo-Aβ surface on β-barrel (see figure); this construct was highly active in inhibiting Aβ oligomerization. One GFP variant (P13H), which mimicked parallel β sheets of Aβ, found bind with high affinity and inhibit oligomerization, even though concentration P13H lower than that Aβ. Supporting information for article is available WWW under...
Some neurodegenerative diseases such as Alzheimer disease (AD) and Parkinson are caused by protein misfolding. In AD, amyloid beta-peptide (Abeta) is thought to be a toxic agent self-assembling into variety of aggregates involving soluble oligomeric intermediates fibrils. Here, we have designed several green fluorescent (GFP) variants that contain pseudo-Abeta beta-sheet surfaces evaluated their abilities bind Abeta inhibit oligomerization. Two GFP P13H AP93Q bound tightly Abeta, K(d) = 260...
In order to realize high-performance LSI systems, a Si interposer with Cu filled through silicon vias (TSVs) and low inductance decoupling capacitor was developed in this work. We fabricated thin film (TFC) on the evaluated its properties. Capacitance density of TFC achieved 2uF/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . Temperature coefficient capacitance (TCC) under 15% (25-125 deg C). The leakage current at 3V below 1nA up...
We have evaluated the feasibility of a telenursing system for patients with chronic condition who are in receipt home care services. The Internet-based allows (equipped laptop computer), nurses and physicians to access information from central database through wireless network (128 kbit/s). Email video-mail messages as well vital signs data can be sent daily by patient server at regional health-care centre, accessed nurse or physician, then decide on appropriate care. was tested male type 2...