A5066580511- Cardiac Valve Diseases and Treatments
- Mechanical Circulatory Support Devices
- Infective Endocarditis Diagnosis and Management
- Hearing, Cochlea, Tinnitus, Genetics
- Cardiovascular Function and Risk Factors
- Health Systems, Economic Evaluations, Quality of Life
- Heart Failure Treatment and Management
- Parkinson's Disease Mechanisms and Treatments
- Cardiac pacing and defibrillation studies
- Vascular Procedures and Complications
- Acute Myocardial Infarction Research
- Connexins and lens biology
- Multiple Sclerosis Research Studies
- COVID-19 and healthcare impacts
- Aortic Disease and Treatment Approaches
- Cardiac Structural Anomalies and Repair
- Atrial Fibrillation Management and Outcomes
- Lipoproteins and Cardiovascular Health
- Congenital Heart Disease Studies
- Healthcare cost, quality, practices
- Cardiac Imaging and Diagnostics
- Cardiac Arrhythmias and Treatments
- Venous Thromboembolism Diagnosis and Management
- Acoustic Wave Phenomena Research
- Voice and Speech Disorders
Providence College
2019-2024
Research Network (United States)
2021-2024
Hope Heart Institute
2020-2024
Providence Health & Services
2017-2023
St. Joseph Health System
2019-2023
Providence Portland Medical Center
2017-2022
Center for Neurosciences
2014-2021
Oregon Health & Science University
2009-2021
Johns Hopkins Hospital
2020
University of Kentucky
2019
Aggregated alpha-synuclein inclusions are found where cell death occurs in several diseases, including Parkinson's disease, dementia with Lewy bodies, and multiple-system atrophy. However, the relationship between inclusion formation an individual cell's fate has been difficult to study conventional techniques. We developed a system that allows for vivo imaging of same neurons over months. show intracerebral injection preformed fibrils recombinant can seed aggregation transgenically...
<h3>Importance</h3> The coronavirus disease 2019 (COVID-19) pandemic has changed health care delivery worldwide. Although decreases in hospitalization for acute myocardial infarction (AMI) have been reported during the pandemic, implication in-hospital outcomes is not well understood. <h3>Objective</h3> To define changes AMI case rates, patient demographics, cardiovascular comorbidities, treatment approaches, and pandemic. <h3>Design, Setting, Participants</h3> This cross-sectional study...
Sound detection by inner ear hair cells requires tip links that interconnect mechanosensory stereocilia and convey force to yet unidentified transduction channels. Current models postulate a static composition of the link, with protocadherin 15 (PCDH15) at lower cadherin 23 (CDH23) upper end link. In terminally differentiated mammalian auditory cells, are subjected sound-induced forces throughout an organism's life. Although can regenerate disrupted restore hearing, molecular details this...
Parkinson's disease and dementia with Lewy bodies are associated abnormal neuronal aggregation of α-synuclein. However, the mechanisms their relationship to poorly understood. We developed an in vivo multiphoton imaging paradigm study α-synuclein mouse cortex subcellular resolution. used a green fluorescent protein-tagged human line that has moderate overexpression levels mimicking disease. Fluorescence recovery after photobleaching (FRAP) labeled protein demonstrated somatic existed...
The quantitative trait locus <i>ahl8</i> is a key contributor to the early-onset, age-related hearing loss of DBA/2J mice. A nonsynonymous nucleotide substitution in mouse fascin-2 gene (<i>Fscn2</i>) responsible for this phenotype, confirmed by wild-type BAC transgene rescue In chickens and mice, FSCN2 protein abundant hair-cell stereocilia, actin-rich structures comprising mechanically sensitive hair bundle, concentrated toward stereocilia tips bundle9s longest stereocilia. expression...
<b><i>Background:</i></b> Rapid evaluation of dysphagia poststroke significantly lowers rates aspiration pneumonia. Logistical barriers often delay in-person by speech language pathologists (SLPs) in remote and rural hospitals. Clinical swallow evaluations delivered via telehealth have been validated a number clinical contexts, yet no one has specifically teleswallow for in-hospital post-stroke assessment. <b><i>Methods:</i></b> A team 6 SLPs...
The curry spice curcumin plays a protective role in mouse models of neurodegenerative diseases, and can also directly modulate aggregation α-synuclein protein vitro, yet no studies have described the interaction genetic synucleinopathy models. Here we examined effect chronic acute treatment Syn-GFP line, which overexpresses wild-type human protein. We discovered that diet intervention significantly improved gait impairments resulted an increase phosphorylated forms at cortical presynaptic...
Phosphorylation of alpha-synuclein at serine-129 is an important marker pathologically relevant, aggregated forms the protein in several human diseases, including Parkinson's disease, Dementia with Lewy bodies, and Multiple system atrophy. Although kinases have been shown to be capable phosphorylating various model systems, identity kinase that phosphorylates body remains unknown. One member Polo-like family, PLK2, a strong candidate for being kinase. To examine this possibility, we used...
Breast cancer brain metastases (BCBM) are a common and devastating complication of metastatic breast with conventional systemic therapies demonstrating limited effectiveness. Consequently, radiotherapy (RT) ± surgery remains the cornerstone BCBM management. Because preclinical clinical evidence indicate that immune checkpoint blockade (ICB) may synergize RT to promote tumor regression, we explored safety efficacy concurrent tremelimumab-mediated cytotoxic T-lymphocyte associated protein 4...
We optimized Fluo-4 AM loading of chicken cochlea to report hair-bundle Ca2+ signals in populations hair cells. The bundle signal reported the physiological state and cell; extruding cells had very high fluorescence, with intact bundles tip links intermediate damaged broken low fluorescence. Moreover, fluorescence correlated entry through transduction channels; mechanically activating channels increased signal, while breaking chelators or blocking each caused cell-body decrease. These...
Measuring the abundance of many proteins over a broad dynamic range requires accurate quantitation. We show empirically that, in MS experiments, relative quantitation using summed dissociation-product ion-current intensities is accurate, albeit variable from protein to protein, and outperforms spectral counting. By applying quantify two complex but related tissues, chick auditory vestibular sensory epithelia, we find that glycolytic enzymes are enriched threefold whereas responsible for...
<h3>Importance</h3> Hospitals are reimbursed based on Diagnosis Related Groups (DRGs), which defined, in part, by patients having 1 or more complications comorbidities within a given DRG family. have made substantial investment efforts to document these and comorbidities. <h3>Objective</h3> To examine temporal trends DRGs with major complication comorbidity, compare findings 2 alternative measures of disease severity, estimate associated changes payment. <h3>Design, Setting,...
Abstract Background Post-infarction ventricular septal defect (PIVSD) is a rare, life-threatening complication of acute myocardial infarction (AMI). Few studies report the use mechanical circulatory support (MCS) for treatment cardiogenic shock in this setting. We describe our experience using microaxial, transvalvular device (Impella, Abiomed, Danvers, MA, USA) as bridge-to-closure PIVSD. Case summary identified 13 patients from two centres with due to PIVSD who received an Impella between...
Stroke produces a region of complete cell death and areas partial damage, injury, gliosis. The spatial relationship these regions damage to the infarct core within spared neuronal circuits has not been identified. A model cortical stroke was developed functional subsets somatosensory cortex. Infarct size, apoptosis, oxidative DNA heat shock protein induction, subtypes reactive gliosis were precisely mapped with body map, quantified, interrelated. Three tissue microenvironments recognized:...
During development of the chick cochlea, actin crosslinkers and barbed-end cappers presumably influence growth remodeling paracrystal hair cell stereocilia. We used mass spectrometry to identify quantify major actin-associated proteins cochlear sensory epithelium from E14 E21, when stereocilia widen lengthen. Tight (<i>i.e.</i> fascins, plastins, espin) are expressed dynamically during between E7 with FSCN2 replacing FSCN1 plastins remaining low in abundance. Capping protein, a capper, is...
Background: Following approval of dimethyl fumarate (DMF), we established a registry relapsing multiple sclerosis (RMS) patients taking DMF at our community MS center. Objective: To track patients’ tolerability, disease progression, and lymphopenia. Methods: Patients prescribed for RMS from March 2013 to 2016 were prospectively enrolled ( N = 412). Baseline data, clinical relapses, magnetic resonance imaging (MRI) activity, discontinuation, lymphocyte counts captured through chart review....
ObjectiveVascular complications (VC) and bleeding impact morbidity mortality after transfemoral transcatheter aortic valve replacement (TF-TAVR). Few contemporary studies have detailed these complications, associated treatment strategies, or clinical outcomes. We examined the incidence, predictors, outcomes of VCs in a multicenter cohort patients undergoing TF-TAVR.MethodsWe performed retrospective registry chart review all nonclinical trial TF-TAVR from seven centers within five-state...
The COVID-19 pandemic has disrupted routine cardiovascular care, with unclear impact on procedural deferrals and associated outcomes across diverse patient populations.Cardiovascular procedures performed at 30 hospitals 6 Western states in 2 large, non-profit healthcare systems (Providence St. Joseph Health Stanford Healthcare) from December 2018-June 2020 were analyzed for changes over time. Risk-adjusted in-hospital mortality was compared phases multivariate logistic regression.Among...