Visible-light-induced photocatalytic decarboxylative alkynylations of carboxylic acids have been developed for the first time. The reaction features extremely mild conditions, broad substrate scope, and avoids additional oxidants. Importantly, a carbonylative alkynylation has also carried out in presence carbon monoxide (CO) under which affords valuable ynones high yields at room temperature.

Batting the ylides: A simple procedure carried out under mild conditions allows direct and efficient synthesis of structurally diverse indoles. This approach involves a cascade reaction sulfur ylides N-(ortho-chloromethyl)aryl amides (see scheme). Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note: The publisher is responsible for content...

Abstract A visible light‐induced photocatalytic intramolecular cyclization of styryl azides has been developed in the presence ruthenium complex Ru(bpy) 3 Cl 2 (0.5 mol%) as photocatalyst at room temperature. The present strategy features operational simplicity well high functional group tolerance, and provides a facile access to various 2‐substituted N ‐free indoles good excellent yields. Importantly, process can employ sunlight light source afford corresponding products without loss...

The radical difunctionalization of alkenes, which involves the simultaneous incorporation two functional groups across a carbon‐carbon double bond, is emerging as robust platform for step‐ and atom‐economical construction valuable molecules from readily accessible starting materials. Given significant importance versatile applications sulfonyl cyano groups, we herein report, first time, visible light‐induced copper‐catalyzed enantioselective sulfonylcyanation reaction vinylarenes. This...

Abstract Enantioselective Friedel–Crafts alkylation reactions of a variety indoles with ethyl glyoxylate, catalyzed by chiral ( S )‐BINOL‐Ti(IV) complex (10 mol %), are reported. The corresponding 3‐indolyl(hydroxy)acetates were formed in good yields and high enantiomeric excess (up to 96 %). When methyl pyruvate or p ‐chlorophenylglyoxal was used, the bisindole compound obtained excellent yield. A possible mechanism is proposed.

Abstract Economical approach : The first organocatalytic asymmetric intramolecular hydroarylation of phenol‐ and aniline‐derived enals offers one the most straightforward atom‐economic approaches to enantioriched chromans tetrahydroquinolines (up 96 % ee ; see scheme). magnified image

Abstract A hydrogen‐bond‐mediated asymmetric [4+1] annulation/rearrangement cascade of stable sulfur ylides and nitroolefins was developed. This reaction provides a facile route to enantioenriched 4,5‐substituted oxazolidinones in moderate excellent isolated yields (65–96 %) with stereocontrol (up more than 95:5 d.r. 97:3 e.r.). methodology successfully applied the concise synthesis two bioactive molecules. The stereocontrolled modes mechanism have been proposed explain origin this stereochemistry.

Abstract Photocatalysis enables the cascade reactions of indoles and CBr 4 in MeOH through a C(sp 2 )H functionalization/methanolysis sequence. The title reaction provides an efficient access to indole 2‐ 3‐carboxylates single operation (no preinstallation protecting as well directing groups was required) with good yields under mild conditions.

Lignocellulose is considered as a good resource for producing renewable energy. Previous in vitro studies have shown the synergistic action between cellulase and xylanase during lignocellulose biohydrolysis. In order to achieve same effect S. cerevisiae enhance practical biotransformation, two recombinant Saccharomyces strains (INVSc1-CBH-CA INVSc1-CBH-TS) with co-expressed were constructed. The activities INVSc1-CBH-CA INVSc1-CBH-TS 716.43 U/mL 205.13 U/mL, 931.27 413.70 respectively. can...

Cellulose is the most important component of lignocellulose, and its degradation requires three different types enzymes to act synergistically. There have been reports single gene duality, but no has described more than two functions. Cloning expression fusion cellulases containing kinds catalytic domains not reported thus far.We synthesized cellulase genes linked with a (G4S)3 flexible linker. The trifunctional (BCE) functions was constructed expressed in S. cerevisiae successfully....

Lignocellulose is a polysaccharide and an abundant biomass resource that widely exists in grains, beans, rice, their by-products. Over 10 million tons of lignocellulose resources processing products are produced every year China. Three recombinant Y. lipolytica strains with cellulase (β-glucosidase, endoglucanase cellobiohydrolase) were constructed. The enzymatic activities these enzymes 14.181 U/mL, 16.307 17.391 respectively. whole cell cellulases used for stover bio-transformation....

Cellulose is an abundant natural polysaccharide that universally distributed. It can be extracted from corncobs, which are inexpensive, easily accessible, renewable, and environmentally friendly. A common strategy for effectively utilizing cellulose efficient heterogeneous expression of cellulase genes in Saccharomyces cerevisiae. However, the improvement utilization a relevant issue. Based on our previous findings, we constructed integrated secretion vector, pHBM368-pgk, containing...

Ustilago maydis infection is a serious disease affecting corn crops worldwide. Sterol 14α-demethylase (CYP51) one of the key enzymes sterol biosynthesis and an effective target antifungal drugs. To further study interaction between CYP51 drugs exploit more specific inhibitor (DMI) fungicides for U. maydis, in this homology modeling from (UmCYP51) templated as eukaryotic orthologues (the human CYP51) screening new DMI based on optimized expression were carried out first time. In addition,...

Abstract An organocatalytic asymmetric conjugate addition of 2‐oxindole‐3‐carboxylate esters to 2‐phthalimidoacrylates catalyzed by a quinine‐derived thiourea is described. The reaction provides efficient access variety quaternary oxindole based C γ ‐tetrasubstituted α‐amino acid derivatives in good excellent yields (up 96 %) with moderate enantioselectivity 90 % ee ). opposite enantiomers the products can also be obtained comparable and stereoselectivity simply using quinidine‐derived catalyst.

BACKGROUND: Sterol 14α-demethylase (CYP51), a key target of azole (DMI) fungicides, can be expressed in both prokaryotes and eukaryotes. Green mould citrus, caused by Penicillium digitatum (Pers.) Sacc., is serious post-harvest disease. To develop specific more effective fungicides against this disease, the characteristics interaction between sterol from P. (PdCYP51) possible new were analysed. The cyp51 gene was cloned under different conditions Escherichia coli (Mig.) Cast. & Chalm.,...

Lignocellulose is considered to be a good resource for producing renewable energy. This paper reports on the effect of three surfactants [polyoxyethylene (80) sorbitan monooleate (POE80), rhamnolipid, and tea saponin] cellulase (CBH/EG/BG) expression Saccharomyces cerevisiae in simultaneous saccharification fermentation (SSF) corncob. In this work, optimal surfactant concentrations yeast growth were 0.1% POE80, 0.05% 0.002% saponin. process SSF, reducing sugar content with POE80 was 13.5%...

Pretreatment can improve the hydrolysis efficiency of cellulose, in which biological pretreatment plays an important role. In present study, we uncovered that Rhodococcus has ability lignin degradation, decompose and serve as a carbon source to meet needs its own growth. We used pretreat corn stalks evaluate effect on cellulose hydrolysis. The concentration reducing sugar produced by stalk after is 2.95 g/L. SEM imaging showed resulted surface be no longer complete, some lamellar structures...

Glucose oxidase (GOD) can be inactivated by hydrogen peroxide (H2O2) produced during glucose oxidation; thus, H2O2 is a competitive inhibitor of GOD. In this mechanism, methionine (Met), sulfur-containing amino acid, oxidized H2O2and converted into sulfoxide, thereby inactivating study, to block GOD oxidation, the two-dimensional structures three mutants were designed with computer-aided analysis, and complex their substrate determined using CDOCKER algorithm. Three mutant GODs wild-type...

Nanomedicine provides promising new methodologies for the treatment of tumors but still faces several limitations, including poor colloidal stability, uncontrollable drug release, and insufficient targeting. Herein, hyaluronic acid (HA) was used to modify surface mesoporous silica nanoparticles (MSNs) via a dynamic-covalent linker, phenylborate ester (PBAE), termed MA. The HA modifier provided enhanced stability hybrid nanoparticles. As expected, MA exhibited an improved biocompatibility...