A5068708983- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Nutrition and Physiology
- Immune Cell Function and Interaction
- Renal and related cancers
- Microbial infections and disease research
- Reproductive Biology and Fertility
- Coccidia and coccidiosis research
- Rabbits: Nutrition, Reproduction, Health
- 3D Printing in Biomedical Research
- Livestock and Poultry Management
- Immune Response and Inflammation
- Molecular Biology Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Infant Nutrition and Health
- Biomedical Ethics and Regulation
Institut Cellule Souche et Cerveau
2015-2025
Inserm
2012-2025
Université Claude Bernard Lyon 1
2010-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2025
Groupe Lépine (France)
2015
Délégation Régionale Auvergne-Rhône-Alpes
2014
Allen Institute for Brain Science
2013
Institute of Zoology
2010
Chinese Academy of Sciences
2010
Institut National de la Recherche Agronomique
1991-2003
A short G1 phase is a characteristic feature of mouse embryonic stem cells (ESCs). To determine if there causal relationship between restriction and pluripotency, we made use the Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) reporter system to FACS-sort ESCs in different cell cycle phases. Hence, appeared be more susceptible differentiation, particularly when self-renewed naïve state pluripotency. Transitions from ground naïve, then primed states pluripotency were associated with...
Abstract Leukemia inhibitory factor (LIF)/STAT3 signalling is a hallmark of naive pluripotency in rodent pluripotent stem cells (PSCs), whereas fibroblast growth (FGF)-2 and activin/nodal required to sustain self-renewal human PSCs condition referred as the primed state. It unknown why LIF/STAT3 alone fails PSCs. Here we show that forced expression hormone-dependent STAT3-ER (ER, ligand-binding domain oestrogen receptor) combination with 2i/LIF tamoxifen allows escape from state enter...
The development of transgenic technologies in monkeys is important for creating valuable animal models human physiology so that the etiology diseases can be studied and potential therapies their amelioration may developed. However, efficiency producing primate animals presently very low, there are few reports success. We have developed an improved methodology production rhesus monkeys, making use a simian immunodeficiency virus (SIV)-based vector encodes EGFP protocol infection...
Post-translational modifications of histone H3 on lysine 9, specifically acetylation (H3K9ac) and tri-methylation (H3K9me3), play a critical role in regulating chromatin accessibility. However, the these lineage segregation mammalian blastocyst remains poorly understood. We demonstrate that di- marks, H3K9me2 H3K9me3, decrease during cavitation expansion rabbit blastocyst. Notably, H3K9me3 levels are particularly low inner cell mass cells at onset formation but increase again just before...
Abstract MHC genes in the chicken are arranged into two genetically independent clusters located on same chromosome. These classical B system and restriction fragment pattern-Y (Rfp-Y), a second cluster of identified recently through DNA hybridization. Because small numbers class I II present both Rfp-Y, might be result duplication an entire chromosomal segment. We subcloned, sequenced, analyzed expression loci mapping to Rfp-Y determine whether should considered either as second, or region...
Abstract pim-1 and pim-3 encode serine/threonine kinases involved in the regulation of cell proliferation apoptosis response to cytokine stimulation. We analyzed by leukemia inhibitory factor (LIF)/gp130/signal transducer activator transcription-3 (STAT3) pathway role Pim-1 Pim-3 mouse embryonic stem (ES) self-renewal. Making use ES cells expressing a granulocyte colony-stimulating factor:gp130 chimeric receptor hormone-dependent signal estrogen (STAT3-ERT2), we showed that expression was...
Summary Not much is known about the molecular and functional features of pluripotent stem cells (PSCs) in rabbits. To address this, we derived characterized 2 types rabbit PSCs from same breed New Zealand White rabbits: 4 lines embryonic (rbESCs), 3 induced (rbiPSCs) that were obtained by reprogramming adult skin fibroblasts. All cell required fibroblast growth factor for their proliferation. rbESC showed properties typically associated with primed pluripotency. The cycle rbESCs had a...
After reprogramming to naive pluripotency, human pluripotent stem cells (PSCs) still exhibit very low ability make interspecies chimeras. Whether this is because they are inherently devoid of the attributes chimeric competency or PSCs cannot colonize embryos from distant species remains be elucidated. Here, we have used different types mouse, human, and rhesus monkey analyzed their rabbit cynomolgus embryos. Mouse embryonic (ESCs) remained mitotically active efficiently colonized host In...
Conventional rabbit embryonic stem cell (ESC) lines are derived from the inner mass (ICM) of pre-implantation embryos using methods and culture conditions that established for primate ESCs. In this study, we explored capacity ICM to give rise ESC similar those utilized generate naive ESCs in mice. On single-cell dissociation fibroblast growth factor 2 (FGF2)-free, serum-supplemented medium, ICMs gave lacking DNA-damage checkpoint G1 phase like mouse ESCs, with a pluripotency gene expression...
We have developed a DNA-based method for defining MHC B system genotypes in chickens. Genotyping by this requires neither prior determination of allele-specific differences nucleotide sequence nor the preparation haplotype-specific alloantisera. Allelic at chicken B-F (class I) and B-L II) loci are detected PCR single-strand conformation polymorphism (SSCP) assays. primer pairs were designed to hybridize specifically with conserved sequences surrounding hypervariable regions within two class...
Rabbit induced pluripotent stem cells (rbiPSCs) possess the characteristic features of primed pluripotency as defined in rodents and primates. In present study, we reprogrammed rbiPSCs using human Krüppel-like factors (KLFs) 2 4 cultured them a medium supplemented with fetal calf serum leukemia inhibitory factor. These (designated rbEKA) were propagated by enzymatic dissociation for at least 30 passages, during which they maintained normal karyotype. This new culturing protocol resulted...
Abstract The pig is an emerging animal model, complementary to rodents for basic research and biomedical agronomical purposes. However despite the progress made on mouse rat models produce genuine pluripotent cells, it remains impossible porcine cell lines with germline transmission. Reprogramming of somatic cells using conventional integrative strategies also unsatisfactory. In present study, we compared outcome both non-integrative reprogramming pluripotency chromosome stability during...
ABSTRACT Despite the growing interest in rabbit model for developmental and stem cell biology, characterization of embryos at molecular level is still poorly documented. We conducted a transcriptome analysis preimplantation from E2.7 (morula stage) to E6.6 (early primitive streak using bulk single-cell RNA-sequencing. In parallel, we studied oxidative phosphorylation glycolysis, analysed active repressive epigenetic modifications during blastocyst formation expansion. generated...
The major histocompatibility complex ( MHC ) sequences of three B 21‐like haplotypes deriving from very different origins including the Red Jungle Fowl Gallus gallus were compared with standard 21 haplotype Scandinavian White Leghorn domesticus . present analysis reveals two cDNA for ‐ F and LB every haplotype, itself. Contrary to expectation, no sequence polymorphism in antigen‐binding domains genes, between investigated haplotypes, was found. relative level class I molecules on surface...
In a Brown Leghorn chicken strain, four endogenous proviral loci have been identified. The DNA mapping data show strong homology between their structures and that of the Rous-associated virus 0 (RAV-0) genome. Two them seem similar to ev 3 6 previously described in White chickens; two others are unknown Leghorns. Using amplification methods, envelope genes these viral partially sequenced. results demonstrate subgroup-specific sequences were largely homologous with those RAV-0.
Induced pluripotent stem cells (iPSCs) are obtained by genetically reprogramming adult somatic via the overexpression of specific genes. The resulting possess same differentiation properties as blastocyst-stage embryonic (ESCs) and can be used to produce new individuals complementation, nuclear transfer cloning, or in vitro fertilization after into male female gametes. Therefore, iPSCs highly valuable for preserving biodiversity and, together with cells, enlarge pool reproductive samples...