A5078022723- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Schizophrenia research and treatment
- Pneumonia and Respiratory Infections
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Biochemical and Structural Characterization
- Psychological Treatments and Disorders
- Plant Pathogenic Bacteria Studies
- Antibiotic Use and Resistance
- Public Health and Social Inequalities
- Insect symbiosis and bacterial influences
- Phosphodiesterase function and regulation
- Biosensors and Analytical Detection
- Genetics and Neurodevelopmental Disorders
- Infection Control in Healthcare
- SARS-CoV-2 detection and testing
- Genetic Associations and Epidemiology
- Plant-Microbe Interactions and Immunity
- Autism Spectrum Disorder Research
- Public Health and Environmental Issues
- Antibiotics Pharmacokinetics and Efficacy
- Infection Control and Ventilation
Universidad El Bosque
2017-2024
Pedagogical and Technological University of Colombia
2009-2013
PCR and its variants (RT-PCR qRT-PCR) are valuable innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, reproducible, generating reliable results that easy analyze. During the COVID-19 pandemic, became gold standard technique detecting SARS-CoV-2 virus allowed confirm infection event, those asymptomatic ones, thus save millions of lives. In-house multiplex tests were developed worldwide detect different viral targets ensure results,...
Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes encode for carbapenemases. Here, we aim characterize platforms involved in dissemination blaKPC-2-positive P. ST235 Colombia. In prospective surveillance study infections adult patients attended five ICUs distant cities Colombia, 58 isolates were recovered, which, 27 (46.6%) resistant...
Abstract Resistance to carbapenems in Klebsiella pneumoniae has been mostly related with the worldwide dissemination of KPC, largely due pandemic clones belonging complex clonal (CC) 258. To unravel bla KPC post-endemic clinical impact, here we describe characteristics 68 patients from a high complexity hospital, and molecular genetic their 139 — K. (KPC- Kp ) isolates. Of 26 that presented relapses or reinfections, 16 had changes resistance profiles isolates recovered recurrent episodes. In...
The aim of this study was to test the plausibility using ΦX174 bacteriophage as a tracer viral aerosols spreading in dental aerosol-generating procedure (AGP) model.ΦX174 (~ 108 plaque-forming units (PFU)/mL) added into instrument irrigation reservoirs and aerosolized during class-IV cavity preparations followed by composite fillings on natural upper-anterior teeth (n = 3) phantom head. Droplets/aerosols were sampled through passive approach that consisted Escherichia coli strain C600...
Carbapenem-resistant Acinetobacter baumannii infections are a major public health problem worldwide, requiring the use of "old" antibiotics such as polymyxin B and E (colistin). However, there is concern regarding emergence isolates resistant to these antibiotics. We report case 64-year-old mestizo man hospitalized in an intensive care unit institution Colombia with identification clinical molecular typing colistin- carbapenem-resistant A. isolate mechanisms resistance colistin not...
The carbapenemase OXA-244 is a derivate of OXA-48, and its detection very difficult in laboratories. Here, we report the identification genomic analysis an Escherichia coli isolate (28Eco12) harboring blaOXA-244 gene identified Colombia, South America. 28Eco12 was during retrospective study, it recovered from patient treated Colombia. complete nucleotide sequence established using PacBio platform. A comparative genomics with other blaOXA-244-harboring strains performed. belonged to type (ST)...
Community-genotype methicillin-resistant Staphylococcus aureus (CG-MRSA) clones are a global concern due to their resistance and increased virulence ability cause infections both hospitalized patients healthy people in the community. Here, we characterize 32 isolates of new CG-MRSA clone. These were identified four cities Colombia, South America. The recovered from different epidemiological prospective studies that conducted several regions Colombia. Molecular characterizations included...
Crop production and trade are two of the most economically important activities in Colombia, viral diseases cause a high negative impact to agricultural sector. Therefore, detection, diagnosis, control, management crucial. Currently, Next-Generation Sequencing (NGS) ‘Omic’ technologies constitute right-hand tool for discovery novel viruses studying virus-plant interactions. This knowledge allows development new diagnostic methods key components infectious processes, which could be used...
ABSTRACT We report the draft genome of a clinical multi-resistant Klebsiella pneumoniae (24Kpn33) isolate, whose (5.7 Mbp) harbored 17 antibiotic resistance genes, including bla KPC-2 . Notably, this gene was mobilized within IncP-6 pCOL-1 plasmid, first genetic platform related to acquisition and dissemination in Pseudomonas aeruginosa
Abstract The com_ylbF domain-containing proteins that inhibit sporulation, competition, and biofilm formation by affecting the activity of Ribonuclease RNase-Y in Bacillus subtilis . Similar Com_YlbF are found Staphylococcus aureus , but their function is yet to be determined. This study investigates role (Qrp/YheA, YmcA, YlbF) S. evaluating impact qrp/yheA ymcA, ylbF gene deletion on formation, PIA/PNAG production, hemolytic capacity. It was three genes produced a decrease slight its Global...
La esquizofrenia es una enfermedad que afecta al 1% de la poblacion mundial y tiene heredabilidad hasta del 80%. El estudio esta afeccion soporta evidencias causa genetica, involucran interaccion entre ultiples genes, lo cual representa una manera compleja de herencia. Las investigaciones geneticas esquizofrenia han contribuido notablemente a la determinacion su etiologia. Existen cuatro enfoques para identificar genes susceptibilidad: los estudios ...
The carbapenemase OXA-244 is a derivate of OXA-48, and its detection very difficult in laboratories. Here we report the identification genomic analysis an Escherichia coli isolate (28Eco12) harbouring blaOXA-244 gene identified Colombia, South America. 28Eco12 was during retrospective study it recovered from patient treated Colombia. complete nucleotide sequence established using PacBio platform. A comparative genomics with other blaOXA-244–harbouring strains performed. belonged to...
La esquizofrenia, enfermedad multifactorial, tiene gran heterogeneidad genética y herencia compleja. En Boyacá, Colombia, se estudió un grupo de 20 pacientes esquizofrénicos (16 hombres cuatro mujeres) establecieron las características socio-demográficas clínicas los factores genéticos precipitantes. Se hicieron estudio citogenético análisis descriptivo variables cualitativas cuantitativas. Hubo predominio del comienzo la en adultos jóvenes (promedio edad el momento aparición: 22,5 años)....
Background: YlbF and YmcA are two essential proteins for the formation of biofilm, sporulation, competence in Bacillus subtilis . In these proteins, a new protein domain called com_ylbF was recently discovered, but its role function has not yet been established. Objective: this study, we identified performed an “ silico ” structural analysis YheA protein, another -containing opportunistic pathogen Staphylococcus aureus. Methods: The search yheA gene using BLAST-P tBLASn algorithms....
Pseudomonas aeruginosa, bacteria ubicua que puede generar infecciones complicadas en pacientes hospitalarios, incrementando los índices de morbimortalidad debido al incremento resistencia a antimicrobianos uso clínico, especial última opción terapéutica como carbapenémicos por la adquisición determinantes través elementos genéticos móviles principalmente. OBJETIVO: Caracterizar el perfil y características moleculares P. aislada adultos con diagnóstico infección tres unidades cuidados...
Staphylococcus aureus pandemic clone USA300 has, in addition to its constitutive arginine catabolism (arc) gene cluster, an mobile element (ACME) carrying another such which gives this advantages colonisation and infection. Gene arcR, encodes oxygen-sensitive transcriptional regulator, is inside ACME downstream of the arc situation may have impact on activation. Different relative expression behaviours are proven here for arcRACME arcACME operon compared ones. We also show that artificially...
Abstract Background Given the complexity and diversity of intestinal microbiome, there is a technical challenge finding best way to study factors that affect quality sample obtain results in precise an complete way. Objective To establish effect storage time under constant cryopreservation conditions (−20°C) stool samples for gastrointestinal microbiome Methods A was distributed 3 fractions (1, 2 3). Time 0 without immediate DNA extraction (1). The 15 (2) 30 (3) days were cryopreserved at...
Abstract Resistance to carbapenems in Klebsiella pneumoniae has been mostly related with the worldwide dissemination of KPC, largely due pandemic clones belonging complex clonal (CC) 258. To unravel blaKPC post-endemic clinical impact, here we describe characteristics 68 patients from a high complexity hospital, and molecular genetic their 139 - K. (KPC-Kp) isolates. Of 26 that presented relapses or reinfections, 16 had changes resistance profiles isolates recovered recurrent episodes. In...