A5058195083- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Supramolecular Self-Assembly in Materials
- Inflammasome and immune disorders
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Carbohydrate Chemistry and Synthesis
- interferon and immune responses
- Electron and X-Ray Spectroscopy Techniques
- Image Processing Techniques and Applications
- RNA regulation and disease
- Porphyrin and Phthalocyanine Chemistry
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- Cell Image Analysis Techniques
- Bacteriophages and microbial interactions
- Optimization and Search Problems
- Advanced Electron Microscopy Techniques and Applications
- Respiratory viral infections research
- Lipid Membrane Structure and Behavior
- DNA and Biological Computing
- Advanced Fluorescence Microscopy Techniques
- Phagocytosis and Immune Regulation
- Molecular Biology Techniques and Applications
- Long-Term Effects of COVID-19
Universität Ulm
2019-2025
Abstract Type I interferon (IFN) signalling is tightly controlled. Upon recognition of DNA by cyclic GMP-AMP synthase (cGAS), stimulator genes (STING) translocates along the endoplasmic reticulum (ER)-Golgi axis to induce IFN signalling. Termination achieved through autophagic degradation or recycling STING retrograde Golgi-to-ER transport. Here, we identify GTPase ADP-ribosylation factor 1 (ARF1) as a crucial negative regulator cGAS-STING Heterozygous ARF1 missense mutations cause...
Tight control of cGAS-STING-mediated DNA sensing is crucial to avoid auto-inflammation. The GTPase ADP-ribosylation factor 1 (ARF1) maintain cGAS-STING homeostasis and various pathogenic ARF1 variants are associated with type I interferonopathies. Functional inhibits STING activity by maintaining mitochondrial integrity facilitating COPI-mediated retrograde trafficking deactivation. Yet the factors governing two distinct functions remained unexplored. Here, we dissect ARF1's dual role a...
A growing number of out-of-equilibrium systems have been created and investigated in chemical laboratories over the past decade. One way to achieve this is create a reaction cycle, which forward driven by fuel backward follows different pathway. Such dissipative networks are still relatively rare, however, most non-enzymatic examples based on carbodiimide-driven generation carboxylic acid anhydrides. In work, we describe network that comprises chemically fueled formation phosphoramidates...
Phagocytosis is a cellular process crucial for recognition and removal of apoptotic cells foreign particles, subsequent initiating an appropriate immune responses. The phagocytosis highly complex involves major rearrangements the cytoskeleton. Due to its complexity importance tissue homoeostasis responses it tightly regulated. Over last decade microRNAs (miRNAs) have emerged as important regulators biological pathways including response by fine tuning expression gene regulatory networks. In...
Infection with the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), causative agent of COVID-19 pandemic, leads to profound remodeling cellular membranes, promoting viral replication and virion assembly. A full understanding this drastic process morphogenesis remains lacking. In study, we applied room temperature transmission electron microscopy (TEM) scanning (STEM) tomography visualize SARS-CoV-2 factory in Vero cells, present our results comparison published cryo-EM studies....
Abstract The aqueous self‐assembly of amphiphiles into aggregates such as micelles and vesicles has been widely investigated over the past decades with applications ranging from materials science to drug delivery. combination characteristic properties nucleic acids is substantial interest mimic biological self‐organization compartmentalization. Herein, we present ribose‐ ribonucleotide‐based investigate their well fundamental reactivity. We found that various types are formed, in size...
The COVID-19 pandemic caused by SARS-CoV-2 presents a global health emergency. Therapeutic options against are still very limited but urgently required. Molecular tweezers supramolecular agents that destabilize the envelope of viruses resulting in loss viral infectivity. Here, we show first-generation tweezers, CLR01 and CLR05, disrupt abrogate To increase antiviral activity, series 34 advanced molecular were synthesized insertion aliphatic or aromatic ester groups on phosphate moieties...
Secondary envelopment of the human cytomegalovirus (HCMV) is a critical but not well-understood process that takes place at cytoplasmic viral assembly complex (cVAC) where nucleocapsids acquire their envelope by budding into cellular membranes containing glycoproteins. Previous studies presented controversial results regarding composition envelope, suggesting trans-Golgi and endosomal origins, as well intersections with exosomal endocytic pathways. Here, we investigated role for secondary...
Secondary envelopment of the human cytomegalovirus (HCMV) is a critical but not well-understood process that takes place at cytoplasmic viral assembly complex (cVAC) where nucleocapsids acquire their envelope by budding into cellular membranes containing glycoproteins. Previous studies presented controversial results regarding composition envelope, suggesting trans-Golgi and endosomal origins, as well intersections with exosomal endocytic pathways. Here, we investigated role for secondary...
High-pressure freezing followed by freeze-substitution is a valuable method for ultrastructural analyses of resin-embedded biological samples. The visualization lipid membranes one the most critical aspects any study and can be especially challenging in high-pressure frozen specimens. Historically, osmium tetroxide has been preferred fixative staining agent lipid-containing structures solutions. However, not only rare expensive material, but also volatile toxic. Here, we introduce use...
Scanning Transmission Electron Microscopes (STEMs) acquire 2D images of a 3D sample on the scale individual cell components. Unfortunately, these can be too noisy to fused into useful structure and facilitating good denoisers is challenging due lack clean-noisy pairs. Additionally, representing detailed difficult even for clean data when using regular grids. Addressing two limitations, we suggest differentiable image formation model STEM, allowing learn joint sensor noise in STEM together...
By Menger's theorem, the maximum number of arc-disjoint paths from a vertex s to t in directed graph equals minimum arcs needed disconnect and t, i.e., size an s-t-cut. The max-flow problem network with unit capacities is equivalent problem. Moreover, min-cut problems form strongly dual pair. We relax disjointedness requirement on paths, allowing them be almost disjoint, meaning they may share up one arc.The resulting disjoint (ADP) asks for k s-t-paths such that any two are disjoint....
ABSTRACT Type I interferon (IFN) signalling is tightly controlled. Upon recognition of DNA by cyclic GMP AMP synthase (cGAS), stimulator genes (STING) translocates along the endoplasmic reticulum (ER)-Golgi axis to induce IFN signalling. Termination achieved through autophagic degradation or recycling STING retrograde Golgi-to-ER transport. Here, we identify GTPase ARF1 as a crucial negative regulator cGAS-STING signaling. Heterozygous missense mutations cause novel type interferonopathy...
Abstract Understanding interaction of viruses with the host organism is crucial for fighting global pandemics as well life-threatening diseases in humans and animals. This understanding requires among other things direct visualization viral structures inside infected cells. Only electron microscopy (EM) provides a sufficient resolution this. Using herpesvirus SARS-CoV-2 an example, this article high-lights EM important versatile method virus research.
Scanning Transmission Electron Microscopes (STEMs) acquire 2D images of a 3D sample on the scale individual cell components. Unfortunately, these can be too noisy to fused into useful structure and facilitating good denoisers is challenging due lack clean-noisy pairs. Additionally, representing detailed difficult even for clean data when using regular grids. Addressing two limitations, we suggest differentiable image formation model STEM, allowing learn joint sensor noise in STEM together...