A5060223691- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Sepsis Diagnosis and Treatment
- Thermal Regulation in Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Dermatology and Skin Diseases
- Inflammasome and immune disorders
- Genomics, phytochemicals, and oxidative stress
- Cytokine Signaling Pathways and Interactions
- Sirtuins and Resveratrol in Medicine
- Genetics, Aging, and Longevity in Model Organisms
- T-cell and B-cell Immunology
- Pancreatic function and diabetes
- melanin and skin pigmentation
- Skin Protection and Aging
- Receptor Mechanisms and Signaling
Jeonbuk National University
2016-2023
Reactive oxygen species (ROS) play a major role in both chronological aging and photoaging. ROS induce skin through their damaging effect on cellular constituents. However, the origins of have not been fully elucidated. We investigated that generation replicative senescent fibroblasts is generated by modulation phosphatidylinositol 3,4,5-triphosphate (PIP3) metabolism. Reduction PTEN protein, which dephosphorylates PIP3, was responsible for maintaining high level PIP3 cells consequently...
Sepsis is a life-threatening multi-organ dysfunction with high mortality caused by the body's improper response to microbial infection. No new effective therapy has emerged that can adequately treat patients sepsis. We previously demonstrated interferon-β (IFN-β) protects against sepsis via sirtuin 1-(SIRT1)-mediated immunosuppression. Another study also reported its significant protective effect acute respiratory distress syndrome, complication of severe sepsis, in human patients. However,...
The molecular mechanism underlying the initiation of somatic cell reprogramming into induced pluripotent stem cells (iPSCs) has not been well described. Thus, we generated single-cell-derived clones by using a combination drug-inducible vectors encoding transcription factors (Oct4, Sox2, Klf4 and Myc) single-cell expansion strategy. This system achieved high efficiency after metabolic epigenetic remodeling. Functional analyses cloned revealed that extracellular signal-regulated kinase (ERK)...
Embryonic stem cells (ESCs) are metabolically distinct from their differentiated counterparts. ESC mitochondria less complex and fewer in number than progeny. However, few studies have examined the proteins responsible for differences mitochondrial structure function between ESCs somatic cells. Therefore, this study, we aimed to investigate these two cell types. We demonstrate that HSP60 is more abundant mouse embryonic fibroblasts. Depletion of inhibited proliferation self-renewal,...
Nicotinamide is used to maturate pancreatic progenitors from embryonic stem cells (ESCs) into insulin-producing (IPCs). It has been known that nicotinamide inhibits the enzymatic activity of SIRT1, an NAD+-dependent deacetylase. Here we show SIRT1 knockdown enhances differentiation human ESCs IPCs. clustering size IPCs and expression genes including c-peptide, pancreas/duodenum homeobox protein 1 (PDX1), insulin, somatostatin, glucagon Nkx6.1 in ESC-derived In addition, We found...
Leukemia inhibitory factor (LIF) is a stem cell growth that maintains self‑renewal of mouse embryonic cells (mESCs). LIF cytokine in the interleukin‑6 family and signals via common receptor subunit gp130 ligand‑specific receptor. causes heterodimerization gp130, activating Janus kinase/STAT MAPK pathways, resulting changes protein phosphorylation. The present study profiled LIF‑mediated phosphorylation mESCs proteomic analysis. treated presence or absence were analyzed two‑dimensional...