Aluminum (Al) toxicity is a primary limitation to crop productivity on acid soils, and rice has been demonstrated be significantly more Al tolerant than other cereal crops. However, the mechanisms of tolerance are largely unknown, no genes underlying natural variation have reported. We screened 383 diverse accessions, conducted genome-wide association (GWA) study, QTL mapping in two bi-parental populations using three estimates based root growth. Subpopulation structure explained 57%...

High-throughput phenotyping of root systems requires a combination specialized techniques and adaptable plant growth, imaging software tools. A custom platform was designed to capture images whole systems, novel tools were developed process analyse these images. The its components are wide range studies using diverse growth (hydroponics, paper pouches, gel soil) involving several species, including, but not limited to, rice, maize, sorghum, tomato Arabidopsis. RootReader2D tool is free...

Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical gene encoding a protein four Armadillo repeats. required for broad-spectrum blast mediated by the NLR R Pi-ta and associated Pi-ta2. expressed constitutively encodes two isoforms are mainly localized in cytoplasm. A base pair deletion within coding region fast neutron-generated mutant line M2354 creates truncated protein, resulting...

Abstract The genetic and physiological mechanisms of aluminum (Al) tolerance have been well studied in certain cereal crops, Al genes identified sorghum (Sorghum bicolor) wheat (Triticum aestivum). Rice (Oryza sativa) has reported to be highly tolerant; however, a direct comparison rice other cereals not reported, the are poorly understood. To facilitate phenotyping rice, high-throughput imaging system root quantification computer program was developed, permitting entire system, rather than...

Single nucleotide polymorphisms (SNPs) are highly abundant, amendable to high-throughput genotyping, and useful for a number of breeding genetics applications in crops. SNP frequencies vary depending on the species populations under study, therefore target SNPs need be carefully selected informative each application. While multiple genotyping systems available rice (Oryza sativa L. its relatives), they their informativeness, cost, marker density, speed, flexibility, data quality. In this we...

Abstract ‘Avant’ (Reg. no. CV‐161; PI 699954), an early‐maturing and high‐yielding long‐grain rice ( Oryza sativa L.) cultivar, was developed by the Louisiana State University Agricultural Center at H. Rouse Caffey Rice Research Station near Crowley, LA, approved for release in 2021. Avant selected from cross ‘Trenasse’//‘Cocodrie’/‘Jefferson’/3/‘Ahrent’/‘Cocodrie’// Cocodrie/‘LaGrue’. It demonstrated good yield potential favorable agronomic characteristics 2017 preliminary trial. From 2018...

Transcription factors (TFs) regulate the expression of other genes to indirectly mediate stress resistance mechanisms. Therefore, when studying TF-mediated resistance, it is important understand how TFs interact with in genetic background. Here, we fine-mapped aluminum (Al) QTL Alt12.1 a 44-kb region containing six genes. Among them ART1, which encodes C2H2-type zinc finger TF required for Al rice. The mapping parents, Al-resistant cv Azucena (tropical japonica) and Al-sensitive IR64...

Aroma is an important grain quality trait in rice, controlled by mutations within the BADH2 gene. The simply inherited, and its importance variety development makes it a practical target for marker-assisted selection applied breeding programs. predominant functional mutation BADH2, 8-bp indel, can be reliably detected using PCR-based assay, but available assays associated genotyping platforms are insufficient large-scale molecular applications not compatible with outsourcing services. We...

Abstract The potential of genomic selection (GS) to increase the efficiency breeding programs has been clearly demonstrated; however, implementation GS in rice ( Oryza sativa L.) limited. In recent years, efforts have begun work toward implementing into Louisiana State University (LSU) Agricultural Center program. One first steps for successful is establish a suitable marker set target germplasm and reliable, cost‐effective genotyping platform capable providing informative data with an...

Abstract ‘CLL17’ (Reg. no. CV‐160; PI 693941), a high‐yielding long‐grain rice ( Oryza sativa L.) cultivar, was developed by the Louisiana State University Agricultural Center at H. Rouse Caffey Rice Research Station near Crowley, LA, and approved for release in 2020. CLL17 contains herbicide‐resistant gene use Clearfield production system. The system is defined as brand of cultivars that are tolerant to imidazolinone herbicides. derived from cross ‘CL131’/‘Trenasse’. It showed good yield...

Core Ideas Genomic data from diverse germplasm used for application in targeted breeding germplasm. Six SNPs identified that can characterize all haplotypes present at SD1 locus rice. Three alleles of the gene US rice including two semidwarf alleles. Two and validated differentiate allele KASP assays designed both use high‐throughput applications. Plant height is an important target ( Oryza sativa L.) programs large effect sd1 makes it a suitable marker‐assisted selection. Although deletion...

Improving drought resistance in crops is imperative under the prevailing erratic rainfall patterns. Drought affects growth and yield of most modern rice varieties. Recent breeding efforts aim to incorporate traits varieties that can be suitable alternative irrigation schemes, such as a (semi)aerobic system, row (furrow-irrigated) rice. The identification quantitative trait loci (QTLs) controlling grain yield, important with high selection efficiency, lead markers facilitate marker-assisted...

Abstract Elevated nighttime temperatures resulting from climate change significantly impact the rice crop worldwide. The ( Oryza sativa L.) plant is highly sensitive to high temperature (HNT) during grain-filling (reproductive stage). HNT stress negatively affects grain quality traits and has a major on value of harvested crop. In addition, along with dimensions determining market classes, appearance determine value. During last few years, there been concern for growers industry over...

Abstract Rice ( Oryza sativa L.) yield and grain quality are highly influenced by the planting date; thus, it is crucial to determine optimal window for an area of cultivation. In this study, date experiments conducted at Louisiana State University (LSU) AgCenter Research Station from 2011 2018 were analyzed quantify effect date, genotype, year on key agronomic traits. Planting was strongly associated with across every year, explaining 55.6% overall phenotypic variation. A clear relationship...

Bacterial panicle blight (BPB) and sheath (SB) are major diseases of rice few cultivars have shown a high level resistance to these diseases. A recombinant inbred line (RIL) population developed from the U.S. Jupiter (moderately resistant) Trenasse (susceptible) was investigated identify loci associated with partial disease BPB SB. Disease phenotypes in SB, as well days-to-heading (DTH) trait, were evaluated field. DTH correlated SB diseases, while positively field trials this RIL...

Abstract Background and objectives To maintain the competitiveness of U.S. long‐grain rice in foreign markets, having translucent whole milled grain is critical. An objective technique to detect chalk, opaque areas grain, will provide breeders industry with an effective tool for developing low‐chalk varieties or agronomic practices that reduce chalk occurrence. Two instruments developed at Center Grain Animal Health Research, Department Agriculture‐Agricultural Research Service (USDA‐ARS), a...

Abstract ‘PVL03’ (Reg. no. CV‐159, PI 699656), a Provisia (BASF) long‐grain rice ( Oryza sativa L.) cultivar, was developed by the Louisiana State University Agricultural Center at H. Rouse Caffey Rice Research Station near Crowley, LA, and approved for release in 2020. PVL03 is third cultivar released as part of System with resistance to herbicide, containing active ingredient quizalofop‐p‐ethyl, an AACase (group 1) herbicide. derived from cross ‘PVL01’/‘Catahoula’. Testing preliminary...

‘PVL01’ (Reg. no. CV‐153, PI 682661), a Provisia (BASF) long‐grain rice ( Oryza sativa L.) cultivar, was developed by the Louisiana State University Agricultural Center at H. Rouse Caffey Rice Research Station (HRCRRS) near Crowley, LA, and approved for release director of Experiment in 2017. PVL01 is first cultivar to be released as part System with resistance herbicide, containing active ingredient quizalofop, an AACase (group 1) herbicide. derived from cross ‘Cheniere’/BASF1‐5 that made...

Abstract The performance of novel genetic combinations under untested environmental scenarios and management practices can be virtually examined using process‐based crop models. Indeed, there has been a long‐standing interest in the modeling community to expand utility models broader germplasm panels (e.g., breeding lines or diversity panels). Yet, is often misalignment between data needed parameterize routinely collected by programs. To address this gap, we leverage dataset from long‐term...

‘CL153’ (Reg. No. CV‐148, PI 677005), a Clearfield (BASF, Ludwigshafen, Germany) long‐grain rice ( Oryza sativa L.) cultivar, was developed by the Louisiana State University Agricultural Center at H. Rouse Caffey Rice Research Station (RRS) near Crowley, LA, and approved for release Director of Experiment in 2015. CL153 derived from cross 9502008‐A//AR‐1188/‘Cocodrie’/3/CFX26/9702128/4/‘Cheniere’ that made 2011 RRS advanced 2013 as an F 3 row selection based on agronomic characteristics....

Abstract Background and objectives Milled rice grain appearance traits, including chalk, determine its grade, price, marketability. These traits are evaluated visually (VI) in the United States by officially trained inspectors at commercial mills. Digital imaging systems (IS) provide an alternative means to objectively rapidly measure these traits. The goals of this study were compare chalk values determined VI IS identify parameters that associated with five commonly assessed US industry....

Abstract Salinity is a major environmental constraint affecting rice production in both irrigated and non‐irrigated areas threatening global food security. Development of salt‐tolerant varieties with desirable agronomic traits logical approach to maintain high yield saline areas. Here, we report development two multi‐parent advanced generation introgression line (MAGIL) populations US cultivar backgrounds their physiological morphological evaluation under salinity stress at germination...

Rice blast, caused by Magnaporthe oryzae, is the most destructive rice disease worldwide. The symptoms are usually expressed on leaf and panicle. intensity in controlled environmental conditions frequently quantified using a 0 to 5 scale, where represents absence of symptoms, large eyespot lesions. However, this scale restricts qualitative classification varieties into intermediate resistant susceptible categories. Here, we develop 6 for blast that allows proper assignment breeding lines six...

Rice grain shape is a major determinant of rice market value and the end-use. We mapped quantitative trait loci (QTL) for traits in bi-parental recombinant inbred line population (Trenasse/Jupiter) discovered two length QTLs-qGL3.1 qGL7.1. Previously, gene GS3 was reported qGL3.1 region GL7 to be encompassing qGL7.1 locus. The re-sequencing SNP data on International Research Institute (IRRI) 3K Genome Project (RGP) panel were obtained from IRRI SNP-Seek database both genes haplotype...