A5026205476- Advanced Glycation End Products research
- Alcohol Consumption and Health Effects
- Biochemical effects in animals
- Protein Interaction Studies and Fluorescence Analysis
- Microplastics and Plastic Pollution
- Mercury impact and mitigation studies
- Glycosylation and Glycoproteins Research
- Muscle metabolism and nutrition
- Recycling and Waste Management Techniques
- Protein Hydrolysis and Bioactive Peptides
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Insect Utilization and Effects
- Folate and B Vitamins Research
- Enzyme Production and Characterization
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and disorders
- Child Nutrition and Feeding Issues
- Infant Nutrition and Health
- biodegradable polymer synthesis and properties
- Growth Hormone and Insulin-like Growth Factors
- Renal function and acid-base balance
- Protein Kinase Regulation and GTPase Signaling
- SARS-CoV-2 detection and testing
- Diabetes and associated disorders
University of Belgrade
2010-2024
University of Belgrade – Faculty of Chemistry
2024
Faculty (United Kingdom)
2014-2016
HUN-REN Institute for Nuclear Research
2003
Contaminating microplastics can interact with food proteins in the matrix and during digestion. This study investigated adsorption of chicken egg protein ovalbumin to polystyrene (PS, 110 260 μm) polyethylene terephthalate (PET, 140 MPs acidic neutral conditions alterations structure. Ovalbumin affinity depended on size (smaller > larger), type (PS PET) pH (pH 3 7). In bulk solution, does not change secondary structure significantly, but induces loosening (at 3) tightening 7) tertiary Formed...
Methylglyoxal is a highly reactive ?-oxoaldehyde with elevated production in hyperglycemia. It reacts nucleophilic Lys and Arg side-chains N-terminal amino groups causing protein modification. In the present study, importance of reaction Cys thiol group methylglyoxal modification, competitiveness this those guanidine groups, time course these reactions their role contribution to cross-linking were investigated. Human bovine serum albumins used as model systems. was found that despite very...
The interaction of polyphenolic molecules with human serum albumin (HSA) could lead to changes in the reactivity HSA Cys34 thiol group (HSA-SH).
HSA carbonylation<italic>in vitro</italic>(with MG) and<italic>in vivo</italic>(diabetes) leads to copper(<sc>ii</sc>) binding affinity decrease and release from copper–HSA complexes depending mainly on the redox state of Cys34-SH group.
Substances containing an α-amino-β-mercapto-ethane pharmacophore may be used as effective methylglyoxal scavengers and inhibitors of protein carbonylation cross-linking.
The capacity of the liver to synthesize insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) may be compromised by alcohol. characteristics IGFBP-3 variants obtained from healthy individuals patients with alcoholic cirrhosis (ALC) were compared. Concanavalin A (Con A) affinity electrophoresis ligand blotting demonstrated that there was a gradual change in carbohydrate properties putative progression ALC stages C. As many as 12 ionic species could distinguished,...
The iso-enzyme profiles of urinary N-acetyl-b-D-glucosoaminidase (NAG) were studied in the workers from a chloro-alkaline electrolysis plant that had been continuously exposed to elemental mercury. same then work break four months and later re-exposed mercury for another five months. activities NAG before after control group 2.099 / 1.03 IU/L; 0.909 0.52 IU/L 1.139 0.35 IU/L, respectively. fraction dominant A form activity total activity, (85.319 5.32%), was almost equal (84.649 2.75%)....
Meat samples were subjected to thermal processing combined with simulated INFOGEST in vitro gastrointestinal (GI) digestion. Protein modifications (PMs) screened commercially available PM-specific antibodies. Specific proteins at 20, 37, 50, and 65 kDa react more than 3 antibodies among meat proteins. Lysine methylation methionine oxidation the most prominent PMs WB. Mass spectrometry confirmed bands ≈20 as allergenic proteins: sarcoplasmic calcium-binding protein oyster, 37 tropomyosin...
Analysis of microplastic (MP) occurrence in commercially relevant species is a prerequisite for food risk assessment. Using standardized methodology, we aimed to investigate MP contamination point-of-sale clams, mussels, and Crustacea shellfish collected from various markets (Belgium, Croatia, Serbia, South Korea). An improved digestion protocol yielded ≥ 99.8% efficiency all analyzed. In total 190 samples analyzed individually by microFTIR, MPs were identified 43.68% the with less than 1...
In this study, a cost-effective sandwich ELISA test, based on polyclonal antibodies, for routine quantification SARS-CoV-2 nucleocapsid (N) protein was developed. The recombinant N produced and used the production of mice rabbit antisera. Polyclonal protein-specific antibodies served as capture detection antibodies. prototype has LOD 0.93 ng/mL LOQ 5.3 ng/mL, with linear range 1.52–48.83 ng/mL. heat pretreatment (56 °C, 1 h) decreased, while 1% Triton X-100 increased analytical sensitivity....
The influence of urinary pigments and urine pH on the spectrophotometric determination N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) activity with 2-methoxy-4-(2'-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide as a substrate was studied. investigation performed human rabbit samples. It found that alkaline values influenced NAG in two ways: 1) decreased due to enzyme instability increase, 2) increased because contribution absorbance 2-methoxy-4-(2'-nitrovinyl)-phenol (MNP) at 505 nm....
It has been verified that serum N-acetyl-?-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes of the sialic acid (SA) content carbohydrate parts NAG A form and its influence on total type 1 diabetes mellitus patients without with secondary complications. forms were isolated, purified characterized from 81 IDDM complications (retinopathy, polyneuropathy nephropathy) 25 healthy persons. The ?-2,6-bound SA isoenzyme patterns form, activities...