A5017446355- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Reproductive tract infections research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Herpesvirus Infections and Treatments
- Toxin Mechanisms and Immunotoxins
- Immune Cell Function and Interaction
- Diphtheria, Corynebacterium, and Tetanus
- Ocular Surface and Contact Lens
- Immunodeficiency and Autoimmune Disorders
- Food Allergy and Anaphylaxis Research
- Metal and Thin Film Mechanics
- Glycosylation and Glycoproteins Research
- Probiotics and Fermented Foods
- Escherichia coli research studies
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Urinary Tract Infections Management
- Ion-surface interactions and analysis
- Botulinum Toxin and Related Neurological Disorders
- Microbial Metabolites in Food Biotechnology
- Transgenic Plants and Applications
- Galectins and Cancer Biology
- Systemic Lupus Erythematosus Research
University of Belgrade
2011-2024
Institute of Virology, Vaccines and Sera “Torlak”
2013-2023
Institute of Chemistry, Technology and Metallurgy
2023
Center for Health, Exercise and Sport Sciences
2017
Vinča Institute of Nuclear Sciences
2008-2011
Institute of Economic Sciences
2008-2011
University of Nis
2008
Institute of Immunology
2004
Military Technical Institute
1997
Background: The global burden of inflammatory bowel diseases (IBDs), including ulcerative colitis and Crohn’s disease, is constantly rising. As IBDs significantly reduce patients’ quality life, prevention efficient treatment are paramount importance. Although the molecular mechanisms underlying IBD pathogenesis still not completely understood, numerous studies indicate essential role oxidative stress in progression diseases. Objective: aim this study was to investigate whether prophylactic...
Purpose.: For the successful topical administration of drugs or vaccines to treat ocular surface diseases, efficient and well-tolerated delivery systems/carriers for conjunctival are crucial in development new treatment strategies. The present study investigated efficiency internalization bacterial ghosts (BGs) produced from probiotic Escherichia coli Nissle 1917 (EcN) by human epithelial (HCjE) cell line, EcN BGs cytotoxicity HCjE cells, vivo uptake guinea pig cells. Methods.: cells was...
Aims: This study focuses on the isolation and characterization of a peptide with bacteriocin‐like properties isolated from Lactobacillus rhamnosus strain 68, previously identified by 16S rRNA gene sequencing originating human gastrointestinal flora. Methods Results: The was supernatant bacteria maintained under restrictive conditions combination ethanol precipitation reversed‐phase chromatography. molecular mass as assessed spectrometry 6433·8 Da. An isoelectric point 9·8 determined 2D‐PAGE....
Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent world's leading cause infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food topical applications. can inhibit infection caused by a variety viruses bacteria. To investigate whether iota-carrageenan (I-C) isolated from red alga Chondrus crispus could prevent infection, we assessed if targeted treatment conjunctival mucosa...
Successful induction of antiphospholipid syndrome (APS) in two different non-autoimmune prone mouse strains, BALB/c and C57BL/6, was achieved by tetanus toxoid (TTd) hyperimmunization using adjuvants (glycerol or aluminium hydroxide), adjuvant pretreatments Complete Freund’s Adjuvant (CFA)). APS had manifestations reproductive pathology C57BL/6 mice: fetal resorption (as a consequence extreme T-cell activation obtained the course pretreatment), lowering fecundity polyclonal B-cell...
Trachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As mechanisms engaged in pathology disease are not fully understood, designing a subunit vaccine specific antigens could improve safety for human use. We propose chlamydia-specific ocular mucosa using particulate carriers, bacterial ghosts (BGs)....
Abstract Thaumatin‐like proteins (TLPs) have been established as a new family of fruit and pollen allergens. The aim this study was to develop two‐site ELISA for the quantification thaumatin‐like kiwi allergen (Act d 2) in kiwifruit extracts kiwifruit‐containing food products. Genomic DNA (gDNA) Act 2 amplified deduced amino acid sequence determined obtain primary structure. purified from extract by HPLC identified Edman degradation MS. Balb/c mice were immunized with production mAbs...
It has already been shown that tetanus toxoid (TTd) hyper-immunization is a suitable experimental method for creating the animal model of antiphospholipid syndrome (APS) in BALB/c mice. The severity APS pathology mice mainly correlates to affinity anti-β 2 glycoprotein I (β GPI) antibodies. In this study we have investigated reproductive induced C57BL/6 by TTd using combination different pretreatments (complete Freund's adjuvant or glycerol) and adjuvants (alhydrogel glycerol). A decrease...
Chitosan, a partially deacetylated polysaccharide derived from chitin, and chitosan-N-acetylcysteine (C-NAC), thiolated chitosan, both show enhanced retention times on the ocular surface when compared to other polymers commonly used in eye drops. To evaluate these compounds as adjuvants for drug delivery or uptake of topically administered conjunctival vaccines, biochemical characteristics were investigated vitro vivo. Human epithelial (HCjE) cells investigate biocompatibility buffered...
Studies on experimental antiphospholipid syndrome (APS) models proved that molecular mimicry between plasma protein beta(2)-glycoprotein I (beta(2)GPI) and structure within micro-organisms or their products, might be a cause for APS. Considering the heterogeneity of polyclonal antibodies (aPLs), it is important to define precise characteristics pathogenic aPLs. To avoid influence polyclonality further analyse connection APS, we produced monoclonal (MAbs) against tetanus toxoid (TTd) tested...
Citation Zivkovic I, Stojanovic M, Petrusic V, Inic-Kanada A, Dimitrijevic L. Induction of APS after TTd hyper-immunization has a different outcome in BALB/c and C57BL/6 mice. Am J Reprod Immunol 2011; 65: 492–502 Problem The antiphospholipid syndrome (APS) is systemic autoimmune disease characterized by vascular thrombosis and/or pregnancy complications (lower fecundity lower litter size), as well an increase anti-β2 glycoprotein I (β2GPI)-specific autoantibody titer. We have investigated...
Background In a quest for needle-free vaccine administration strategy, we evaluated the ocular conjunctiva as an alternative mucosal immunization route by profiling and comparing local systemic immune responses to subcutaneous or conjunctival of tetanus toxoid (TTd), model antigen. Materials methods BALB/c C57BL/6 mice were immunized either subcutaneously with TTd alone via alone, mixed 2% glycerol merthiolate-inactivated whole-cell B. pertussis (wBP) adjuvants. Mice on days 0, 7 14 both...
Recent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving way for new possibilities to oppose societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate risks associated with use a whole-cell vaccine. Our rationale design an efficient against Chlamydia trachomatis (Ct) is based on membrane proteins involved initial Ct-host cell contact and route...