A5048834963- Food Allergy and Anaphylaxis Research
- Protein Hydrolysis and Bioactive Peptides
- Proteins in Food Systems
- Metabolism, Diabetes, and Cancer
- Growth Hormone and Insulin-like Growth Factors
- Pancreatic function and diabetes
- Phytase and its Applications
- Biochemical and biochemical processes
- Probiotics and Fermented Foods
- Polyamine Metabolism and Applications
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Quality and Safety in Healthcare
- Allergic Rhinitis and Sensitization
- Protein purification and stability
- SARS-CoV-2 and COVID-19 Research
- Contact Dermatitis and Allergies
- Eosinophilic Esophagitis
- Thyroid Disorders and Treatments
- Consumer Attitudes and Food Labeling
- Transgenic Plants and Applications
- Healthcare and Environmental Waste Management
- Enzyme-mediated dye degradation
- Indoor Air Quality and Microbial Exposure
- Animal health and immunology
University of Belgrade
2010-2023
Rhön-Klinikum
2022
University of Kragujevac
2021
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2018-2020
University of Belgrade – Faculty of Chemistry
2016-2018
Zdravstveni centar
2016
Plovdiv University
2016
Faculty (United Kingdom)
2016
Abstract Conglutins represent the major peanut allergens and are renowned for their resistance to gastro-intestinal digestion. Our aim was characterize digestion-resistant peptides (DRPs) of conglutins by biochemical biophysical methods followed a molecular dynamics simulation in order better understand basis food protein allergenicity. We have mapped proteolysis sites at N- C-termini limited internal segment, while other potential remained unaffected. Molecular showed that only occurred...
Crosslinking enzymes are frequently used in bioprocessing of dairy products. The aim this study was to examine the effects enzymatic crosslinking on IgE binding, allergenicity and digestion stability beta-casein (CN). beta-CN crosslinked by transglutaminase, tyrosinase, mushroom tyrosinase/caffeic acid laccase/caffeic acid. binding compared vitro CAP inhibition assay, ELISA as well ex vivo basophil activation assay. Crosslinked CNs were digested simulated gastric fluid for 15 60 min obtained...
Desiccation, an extreme form of dehydration, reduces the cellular water content to below 5 % and poses a major challenge for most plants. Ramonda serbica, tertiary relict homoiochlorophyllous resurrection plant, is exceptional model investigating vegetative desiccation tolerance. Late Embryogenesis Abundant (LEA) proteins are strongly involved in this adaptive trait, but their exact molecular function still unclear. In study, we report first successful recombinant production...
Summary Background Most food allergens sensitizing via the gastrointestinal tract are stable proteins that resistant to pepsin digestion, in particular major peanut allergens, Ara h 2 and 6. Survival of their large fragments is essential for capacity. However, immunoreactive proteins/peptides which immune system exposed during digestion unknown. Particularly, IgE reactivity short digestion‐resistant peptides (SDRPs; <10 kDa) released by gastric under standardized physiologically relevant...
Scope The cross‐linking of proteins by enzymes to form high‐molecular‐weight protein, aggregates can be used tailor the technological or physiological functionality food products. Aggregation dietary processing may promote allergic sensitization, but effects enzymatic on allergenic potential are not known. In this study, bioavailability and sensitizing tolerizing peanut ( PE ) cross‐linked with microbial tyrosinase from T richoderma reesei mushroom A garicus bisporus , were investigated....
Information on how insulin and insulin-like growth factors 1 2 (IGF-1 -2) activate receptors (IR-A -B) the IGF-1 receptor (IGF-1R) is crucial for understanding difference in biological activities of these peptide hormones. Cryo-EM studies have revealed that uses its binding sites to interact with IR-A identified several critical residues site 2. However, mutagenesis suggest Ile-A10, Ser-A12, Leu-A13, Glu-A17 also belong insulin's Here, resolve this discrepancy, we mutated equivalent IGFs....
Laccase cross-linking of peanut protein causes changes in the structure, phenolic composition and immunological properties treated protein.
Insulin-like growth factors 1 and 2 (IGF-1 -2, respectively) are protein hormones involved not only in normal development but also life span regulation cancer. They exert their functions mainly through the IGF-1R or by binding to isoform A of insulin receptor (IR-A). The IGF-1 IGF-2 antagonists is great clinical interest. Mutations A4 A8 sites human lead disproportionate effects on hormone IR activation. Here, we systematically modified 45, 46, 49 45 48, which correspond, close, A8. IR-A...
The aim of this study was to identify short digestion-resistant peptides (SDRPs) released by pepsin digestion the whole cow’s milk and examine their IgE reactivity allergenicity. Raw subjected simulated gastric digestion. SDRPs were fractionated from digests identified MS. Milk evaluated for aggregability, propensity compete binding with individual allergens, ability bind IgG4 allergic milk-tolerant individuals. majority originated caseins (97% peptides) overlapped known epitopes allergens....
BACKGROUND: The major peanut allergens are Ara h 1, 2 and 6. Proteolytic processing has been shown to be required for the maturation process of aim this study was examine whether undergoes proteolytic and, if so, influences its ability bind human immunoglobulin E (IgE). RESULTS: isolated from extract under conditions protease inhibition revealed a single additional peak two known isoforms (Ara 2.01 2.02), corresponding C-terminally truncated form lacking dipeptide (RY). in absence...
Insulin-like growth factors 2 and 1 (IGF2 IGF1) insulin are closely related hormones that responsible for the regulation of metabolic homeostasis, development organism. Physiological functions IGF1 relatively well-studied, but information about role IGF2 in body is still sparse. Recent discoveries called attention to emerging brain, where it could be involved processes learning memory consolidation. It was also proposed these mediated by receptor (IGF2R). Nevertheless, little known mechanism...
Background: The world production of whey was estimated to be more than 200 million tons per year. Although is an important source proteins with high nutritional value and biotechnological importance, it still considered as a by-product the dairy industry low economic due industrial exploitation. There are several challenges in separation proteins: concentration, complexity material similar properties (pI, molecular mass) some proteins. Methods: A narrative review all relevant papers on...
Resistance to digestion by digestive proteases represents a critical property of many food allergens. Recently, harmonized INFOGEST protocol was proposed for solid digestion. The proposes conditions suitable all kinds and liquid foods. However, peanuts, as lipid-rich food, represent challenge downstream analyses the digestome. This is particularly reflected in methodological difficulties analyzing proteins peptides presence lipids. Therefore, removal lipids seems be prerequisite analysis...
Abstract Almost all known food allergens that sensitize via the gastrointestinal system belong to prolamin and cupin protein superfamilies of allergens. These are mainly characterized by resistance heat digestion. Though appealing, hypothesis allergenicity is directly linked stability pepsin digestion remains controversial. Besides allergen structure, new data emerging on influence various other factors survival uptake gut immune system, some them related properties matrix, others way we...
Structural details of changes accompanying interaction between insulin-related hormones and their binding partners are often enigmatic. Here, cross-linking/mass spectrometry could complement structural techniques reveal these protein-protein interfaces. We used such approach to clarify missing description the interface in human insulin-like growth factor (IGF-1):Drosophila melanogaster imaginal morphogenesis protein-late 2 protein (Imp-L2) complex which we studied previously by X-ray...
In this study, a cost-effective sandwich ELISA test, based on polyclonal antibodies, for routine quantification SARS-CoV-2 nucleocapsid (N) protein was developed. The recombinant N produced and used the production of mice rabbit antisera. Polyclonal protein-specific antibodies served as capture detection antibodies. prototype has LOD 0.93 ng/mL LOQ 5.3 ng/mL, with linear range 1.52–48.83 ng/mL. heat pretreatment (56 °C, 1 h) decreased, while 1% Triton X-100 increased analytical sensitivity....