A CBL-interacting protein kinase (CIPK) gene, BnCIPK6, was isolated in Brassica napus. Through yeast two-hybrid screening, 27 interaction partners (including BnCBL1) of BnCIPK6 were identified Interaction and BnCBL1 further confirmed by BiFC (bimolecular fluorescence complementation) plant cells. Expressions significantly up-regulated salt osmotic stresses, phosphorous starvation, abscisic acid (ABA). Furthermore, promoter activity intensively induced cotyledons roots under NaCl, mannitol,...

Abstract Arabinogalactan proteins (AGPs) are involved in many aspects of plant development. In this study, biochemical and genetic approaches demonstrated that AGPs abundant developing fibers may be fiber initiation elongation. To further investigate the role during development, a fasciclin-like arabinogalactan protein gene (GhFLA1) was identified cotton (Gossypium hirsutum). Overexpression GhFLA1 promoted elongation, leading to an increase length. contrast, suppression expression slowed...

Summary Cotton ( Gossypium hirsutum ) fibers are the highly elongated and thickened single‐cell trichomes on seed epidermis. However, little is known about molecular base of fiber cell wall thickening in detail. In this study, a cotton NAC transcription factor (GhFSN1) that specifically expressed secondary (SCW) was functionally characterized. The GhFSN1 transgenic plants were generated to study how FSN1 regulates SCW formation. Up‐regulation expression resulted an increase thickness but...

PHOSPHATE STARVATION RESPONSE1 (PHR1) is a key regulatory component of the response to phosphate (Pi) starvation. However, regulation PHR1 in this remains poorly understood. Here, we report that target transcription factors AUXIN RESPONSE FACTOR7 (ARF7) and ARF19 positively regulated by auxin signaling Arabidopsis (Arabidopsis thaliana) roots. expression was induced exogenous suppressed transport inhibitors In promoter, three auxin-response elements, which are bound directly ARF7 ARF19, were...

Abstract As the important source of natural fibers in textile industry, cotton fiber quality and yield are often restricted to drought conditions because most plants world grow regions with water shortage. WRKY transcription factors regulate multiple plant physiological processes, including stress response. However, little is known how genes respond cotton. Our previous study revealed GhWRKY33 leaf-specific induced by stress. In this study, our data showed protein localizes cell nucleus able...

Abstract Cotton, one of the most important crops in world, produces natural fiber materials for textile industry. WRKY transcription factors play roles plant development and stress responses. However, little is known about whether how regulate cotton so far. In this study, we show that a fiber-preferential factor, GhWRKY16, positively regulates initiation elongation. GhWRKY16-silenced transgenic displayed remarkably reduced number protrusions on ovule shorter fibers compared to wild-type....

Phytohormones play indispensable roles in plant growth and development. However, the molecular mechanisms underlying phytohormone-mediated regulation of fiber secondary cell wall (SCW) formation cotton (Gossypium hirsutum) remain largely underexplored. Here, we provide mechanistic evidence for functional interplay between APETALA2/ethylene response factor (AP2/ERF) transcription GhERF108 auxin factors GhARF7-1 GhARF7-2 dictating ethylene-auxin signaling crosstalk that regulates SCW...

Abstract Each fiber of cotton (Gossypium hirsutum) is a single epidermal cell that rapidly elongates to 2.5 3.0 cm from the ovule surface within about 16 d after anthesis. A large number genes are required for differentiation and development, but so far, little known how these control regulate process development. To investigate gene expression patterns in fiber, cDNA, GhTUB1, encoding β-tubulin was isolated cDNA library. The analyses RNA northern-blot hybridization reverse...

Dirigent super-family abounds throughout the plant kingdom, especially vascular plants. To elucidate function of cotton (Gossypium hirsutum) DIR genes in lignification, two cDNAs (designated GhDIR1 and GhDIR2) encoding putative dirigent proteins were isolated from cDNA libraries. Real-time quantitative reverse transcription-polymerase chain reaction analysis revealed that transcript was preferentially accumulated hypocotyls, whereas GhDIR2 predominantly expressed fibers. Overexpression gene...

Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPHR1 encoding a MYB transcription activator was isolated from Brassica napus. The characterization nuclear localization and activation ability suggest transcriptional activator. tissue expression histochemical assay showed that predominantly expressed in roots modulated by exogenous Pi level under deficiency conditions. Furthermore, overexpression both Arabidopsis B. napus remarkably enhanced...

Summary Aquaporins are thought to be associated with water transport and play important roles in cotton ( Gossypium hirsutum ) fibre elongation. Among aquaporins, plasma membrane intrinsic proteins PIP s) constitute a plasma‐membrane‐specific subfamily further subdivided into 1 2 groups. In this study, four fibre‐preferential GhPIP2 genes were functionally characterized. The selective interactions among GhPIP2s their interaction studied detail elucidate the molecular mechanism of...

Proteins of the 14-3-3 family regulate a divergent set signalling pathways in all eukaryotic organisms. In this study, several cDNAs encoding proteins were isolated from cotton fibre cDNA library. The Gh14-3-3 genes share high sequence homology at nucleotide level coding region and amino acid level. Real-time quantitative RT-PCR analysis indicated that expression these is developmentally regulated fibres, reached their peak stage rapid cell elongation development. Furthermore, overexpression...

In this study, the GhKNL1 (KNOTTED1-LIKE) gene, encoding a classical class II KNOX protein was identified in cotton (Gossypium hirsutum). preferentially expressed developing fibres at stage of secondary cell wall (SCW) biosynthesis. localized nucleus, and could interact with GhOFP4, as well AtOFP1, AtOFP4, AtMYB75. However, lacked transcriptional activation activity. Dominant repression affected fibre development cotton. The expression levels genes related to elongation SCW biosynthesis were...

Summary Cotton ( Gossypium hirsutum ) fibre is an important natural raw material for textile industry in the world. Understanding molecular mechanism of development future cotton varieties with superior quality. In this study, overexpression Gh14‐3‐3L promoted elongation, leading to increase mature length. contrast, suppression expression , Gh14‐3‐3e and Gh14‐3‐3h slowed down initiation elongation. As a result, fibres Gh14‐3‐3 RNA i transgenic plants were significantly shorter than those...

Arabinogalactan proteins (AGPs) are highly glycosylated that play pivotal roles in diverse developmental processes plants. Type-II AG glycans, mostly O-linked to the hydroxyproline residues of protein backbone, account for up 95% w/w AGP, but their functions still largely unclear. Cotton fibers extremely elongated single-cell trichomes on seed epidermis; however, little is known molecular basis governing regulation fiber cell development. Here, we characterized role a CAZy...

Basic helix-loop-helix/helix-loop-helix (bHLH/HLH) transcription factors play important roles in plant development. Many reports have suggested that bHLH/HLH proteins participate brassinosteroid (BR) hormone signaling pathways to promote cell elongation. Cotton fibers are single-cells and derived from seed surface. To explore the of cotton fiber development progress by modulating BR pathway, we performed a systematic analysis gene family upland (Gossypium hirsutum) genome.In this study,...

Basic helix-loop-helix/helix-loop-helix (bHLH/HLH) transcription factors play important roles in cell elongation plants. However, little is known about how bHLH/HLH antagonistically regulate fiber cotton (Gossypium hirsutum). In this study, we report that two factors, fiber-related protein 2 (GhFP2) and ACTIVATOR FOR CELL ELONGATION 1 (GhACE1), function development of cotton. GhFP2 an atypical bHLH without the basic region, its expression regulated by brassinosteroid (BR)-related...

Fasciclin‐like arabinogalactan proteins (FLAs), a subclass of (AGPs), are usually involved in cell development plants. To investigate the expression profiling as well role FLA genes fiber development, 19 GhFLA (cDNAs) were isolated from cotton ( Gossypium hirsutum ). Among them, 15 predicted to be glycosylphosphatidylinositol anchored plasma membranes. The FLAs could divided into four groups. Real‐time quantitative reverse transcriptase polymerase chain reaction results indicated that...

Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in mediating biotic and abiotic stress responses. Cotton (Gossypium hirsutum) is the most important textile crop world, often encounters during its growth seasons. In this study, a gene encoding mitogen-activated was isolated from cotton, designated as GhMPK17. The open reading frame (ORF) of GhMPK17 1494 bp length encodes with 497 amino acids. Quantitative RT-PCR analysis indicated that expression up-regulated cotton under...