A5087878572- Photoreceptor and optogenetics research
- Orbital Angular Momentum in Optics
- Microfluidic and Bio-sensing Technologies
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Digital Holography and Microscopy
- Advanced Fluorescence Microscopy Techniques
- Near-Field Optical Microscopy
- Molecular Communication and Nanonetworks
- Ocular and Laser Science Research
- Optical Coherence Tomography Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Laser-Ablation Synthesis of Nanoparticles
- Carbon Nanotubes in Composites
- 3D Printing in Biomedical Research
- Erythrocyte Function and Pathophysiology
- Photonic and Optical Devices
- Laser Material Processing Techniques
- Laser Applications in Dentistry and Medicine
- Optical Imaging and Spectroscopy Techniques
- Axon Guidance and Neuronal Signaling
- bioluminescence and chemiluminescence research
- Advanced X-ray Imaging Techniques
Scope Group (United States)
2015-2025
Deutsches Elektronen-Synchrotron DESY
2019
Transcend Vivoscope (China)
2019
The University of Texas at Arlington
2011-2016
Beckman Laser Institute and Medical Clinic
2007-2012
University of California, Irvine
2007-2012
University of Electronic Science and Technology of China
2012
University of California, San Diego
2009
Raja Ramanna Centre for Advanced Technology
2001-2008
Indian Institute of Science Bangalore
2005-2008
Proper recognition and repair of DNA damage is critical for the cell to protect its genomic integrity. Laser microirradiation ranging in wavelength from ultraviolet A (UVA) near-infrared (NIR) can be used induce a defined region nucleus, representing an innovative technology effectively analyze vivo double-strand break (DSB) process mammalian cells. However, damage-inducing characteristics different laser systems have not been fully investigated. Here we compare nanosecond nitrogen 337 nm...
Cumulative evidence from both humans and animals suggests that the anterior cingulate cortex (ACC) is important for pain-related perception, thus a likely target pain relief therapy. However, use of existing electrode based ACC stimulation has not significantly reduced pain, at least in part due to lack specificity co-activation excitatory inhibitory neurons. Herein, we report dramatic reduction behavior transgenic mice by optogenetic neural circuitry expressing channelrhodopsin-2....
We report measurement of optical transport parameters normal and malignant (ductal carcinoma) human breast tissue. A spatially resolved steady-state diffuse reflectance technique was used for the reduced scattering coefficient (μs′) absorption (μa) The anisotropy parameter (g) estimated by goniophotometric measurements phase function. values μs′ μa tissue were observed to be larger than those over wavelength region investigated (450–650 nm). Further, using both measurements, we Mie...
In this letter, we developed an integrated neural probe prototype for optogenetic stimulation by microscale light-emitting diode (μLED) and simultaneous recording of activities with microelectrodes on a single-polyimide platform. Optogenetics stimulates in vivo circuits high-cellular specificity achieved genetic targeting precise temporal resolution interaction light-gated ion channels optical beam. our newly optrode probe, during neurons, continuous sensing neuronal vicinity the activation...
Optogenetics is an innovative technique for optical control of cells. This field has exploded over the past decade or so and given rise to great advances in neuroscience. A variety applications both from basic applied research have emerged, turning early ideas into a powerful paradigm cell biology, neuroscience, medical research. review aims at highlighting concepts that are essential comprehensive understanding optogenetics some important biological/biomedical applications. Further,...
Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile a transparent object with sub-wavelength accuracy. Here, we report quantitative phase laser microsurgery system for evaluation cellular/ sub-cellular during micro-dissection. The proposed method takes advantage precise manipulation by microbeam and imaging digital high spatial temporal resolution. This will permit damage and/or repair cell or organelles real time.
Mohanty, S. K., Rapp, A., Monajembashi, S., Gupta, P. K. and Greulich, O. Comet Assay Measurements of DNA Damage in Cells by Laser Microbeams Trapping Beams with Wavelengths Spanning a Range 308 nm to 1064 nm. Radiat. Res. 157, 378–385 (2002).DNA damage induced NC37 lymphoblasts optical tweezers continuous-wave Ti:sapphire laser Nd:YAG (60–240 mW; 10–50 TJ/m2; 30–120 s irradiation) was studied the comet assay, single-cell technique used detect fragmentation genomes. Over wavelength range...
We report observation of optical binding between two dielectric particles with dimensions less than the wavelength interacting light. The observed dependence separation optically bound Rayleigh on polarization trapping beam is in agreement earlier theoretical predictions.
The short working distance of microscope objectives has severely restricted the application optical micromanipulation techniques at larger depths. We show first use fiber-optic tweezers toward controlled guidance neuronal growth cones and stretching neurons. Further, by mode locking, beam was converted to scissors, enabling dissection processes thus allowing study subsequent response neurons localized injury. At high average powers, lysis a three-dimensionally trapped cell accomplished.
It has recently been shown that a red blood cell (RBC) can be used as optically driven motor. The mechanism for rotation is however not fully understood. While the dependence on osmolarity of buffer led us to conclude dependent changes in shape are responsible observed rotation, role ion gradients and folding RBC rod invoked by Dharmadhikari et al explain their observations. In this paper we report results studies undertaken understand dynamics when it tweezed. obtained support our earlier...
We demonstrate the use of a single fiber-optic axicon device for organization microscopic objects using longitudinal optical binding. Further, by manipulating shape fiber tip, part emanating light was made to undergo total internal reflection in conical tip region, enabling near-field trapping. Near-field trapping resulted and self-organization long chains particles along azimuthal directions (in contrast axial direction, observed case large cone angle far-field trapping).
Methods of controllable, noncontact rotation optically trapped microscopic objects have garnered significant attention for tomographic imaging and microfluidic actuation. Here, we report development a fiber-optic spanner demonstrate controlled smooth muscle cells. The is realized by introducing transverse offset between two counterpropagating beams emanating from single-mode optical fibers. speed surrounding flow could be varying balanced laser beam powers. Further, simultaneous translation...
We demonstrate that a focused beam through microaxicon built on the tip of single mode optical fiber can trap low-index objects at much larger depths as compared to vortex tweezers generated using high numerical aperture microscope objectives. The measured transverse trapping force for in Mie regime was found depend particle size and distance trapped from tip. While axial movement achieved by variation power, transportation three dimensions maneuvering fiber.
The laser microbeam has enabled highly precise noncontact delivery of exogenous materials into targeted cells without compromising cell viability, which been a challenging task for traditional methods. Here, we report impermeable substances mammalian and goldfish retinal explants subsequent to ultrafast assisted injection. Introduction dye the through localized pore formation was confirmed by distinct fluorescence at site on membrane its spatiotemporal diffusion pattern nucleus. Indirect...