Lung cancer is the first leading cause of deaths worldwide. Non‐small cell lung (NSCLC) most common type cancer. Increasing evidence shows that long noncoding RNA (lncRNA) are capable modulating tumor initiation, proliferation and metastasis. In present study, we aimed to evaluate whether circulating lncRNA could be used as biomarkers for diagnosis prognosis NSCLC. Expression profiles 14 selected from other studies were validated in 20 pairs tissues by quantitative real‐time PCR,...

// Weili Duan 1, * , Lutao Du Xiumei Jiang 1 Rui Wang Suzhen Yan Yujiao Xie Keqiang 2 Qingliang 3 Lili Xin Zhang Hongwei Pan Yongmei Yang Chuanxin Department of Clinical Laboratory, Qilu Hospital Shandong University, Jinan, 250012, Province, China Urology, Medical Affairs Management, These authors contributed equally to this work Correspondence to: Wang, email: cxwang@sdu.edu.cn Keywords: lncRNA, bladder cancer, diagnosis, recurrence, serum Received: July 25,...

Abstract Exosomes are small membrane vesicles released by many cells. These can mediate cellular communications transmitting active molecules including long non‐coding RNA s (lnc RNAs ). In this study, our aim was to identify a panel of lnc in serum exosomes for the diagnosis and recurrence prediction bladder cancer ( BC The expressions 11 candidate exosome were investigated training set (n = 200) an independent validation 320) via quantitative real‐time PCR . A three‐lnc PCAT ‐1, UBC 1 SNHG...

Abstract Some types of long noncoding RNAs (lncRNAs) are aberrantly expressed in human diseases, including cancer. However, the overall biological roles and clinical significances most lncRNAs colorectal cancer (CRC) not fully understood. First, The Cancer Genome Atlas (TCGA) was analyzed to identify differentially between CRC tissues noncancerous tissues. We identified that LINC02418 highly cell lines. Next, we evaluated effect on tumorigenesis its regulatory functions absorbing microRNA...

Exosomes have emerged as potential biomarkers for pancreatic cancer (PaC). However, it is still challenging to get quantitative detection of exosomes with the specific surface receptors. In this study, a highly sensitive system first constructed direct quantitation in real samples using hierarchical surface-enhanced Raman scattering substrate (H-SERS substrate) and rapid enrichment strategy magnetic beads @ SERS probes (MEDP). It found that (MEDP H-SERS could provide 3.5 times higher...

// Lutao Du 1 , Xiumei Jiang Weili Duan Rui Wang lishui Guixi Zheng Keqiang Yan 2 Lili Juan Li Xin Zhang Hongwei Pan Yongmei Yang and Chuanxin 3 Department of Clinical Laboratory, Qilu Hospital Shandong University, Jinan, 250012, Province, China Urology, The Second Jinan 250033, Shandong, Correspondence to: Wang, email: cxwang@sdu.edu.cn Keywords: microRNA, bladder cancer, diagnosis, recurrence, urine Received: October 22, 2016     Accepted: March 14,...

Noninvasive biomarkers for predicting the risk of muscle-invasive bladder cancer (MIBC) may expedite appropriate therapy and reduce morbidity cost. Genome-wide miRNA analysis by Miseq sequencing followed two phases reverse transcription quantitative real-time PCR (RT-qPCR) assays were performed on serum from 207 MIBC patients, 285 nonmuscle-invasive (NMIBC) patients 193 controls. A four-miRNA panel (miR-422a-3p, miR-486-3p, miR-103a-3p miR-27a-3p) was developed prediction with an area under...

Abstract Cell‐free long non‐coding RNAs (lncRNAs) are stably present in urine and can serve as non‐invasive biomarkers for cancer. We aimed to identify signatures of lncRNAs diagnosis prognosis bladder cancer (BC). Screening by microarray analysis was performed using samples 10 BC patients controls. Expressions candidate were evaluated the training validation set including 230 controls quantitative reverse transcription polymerase chain reaction (qRT‐PCR). A two‐lncRNA panel (uc004cox.4...

Breast cancer is the major cause of death worldwide in women. Patients with metastasis have poor prognosis and mechanisms breast are not completely understood. Long non-coding RNAs (lncRNAs) been shown to crucial roles development progression. However, underlying by which lncRNA-driven unknown. The main objective this paper explore a functional lncRNA its cancer. Here we identified novel AC073352.1 that was significantly upregulated tissues associated advanced TNM stages patients. In...

Bladder cancer (BCa) is a heterogeneous disease with various tumorigenic mechanisms and clinical behaviors.The current tumor-node-metastasis (TNM) staging system inadequate to predict overall survival (OS) in BCa patients.We developed BCa-specific, long-non-coding-RNA (lncRNA)-based nomogram improve prediction BCa.We obtained the large-scale gene expression profiles of samples from 414 patients The Cancer Genome Atlas database.Using an lncRNA-mining computational framework, we identified...

MiR-210 is the master hypoxamir that generally exhibits oncogenic properties in most human solid tumors including bladder cancer (BC). However, it remains unknown about clinical significance of circulating miR-210 levels BC. In this study, we found serum was up-regulated patients with BC, and increased advancing stage grade. Moreover, expression to be significantly reduced paired post-operative samples elevated relapsed Taken together, our data suggest could a potential noninvasive biomarker...

Exosomal long non-coding RNAs (lncRNAs) have been recognised as promising stable biomarkers in cancers. The aim of this study was to identify an exosomal lncRNA panel for diagnosis and prognosis esophageal squamous cell carcinoma (ESCC).Exosomes were isolated from serum by ExoQuick Solution. To validate the exosomes, markers characterization nanoparticle performed. Quantitative real-time PCR used measure levels lncRNAs exosomes ESCC patients healthy subjects. In training set, profiles 404...

// Xiumei Jiang 1, * , Wenfei Wang 2, Yongmei Yang 3 Lutao Du Xiaoyun Lili Guixi Zheng Weili Duan 1 Rui Xin Zhang Lishui Xiaoyang Chen 2 and Chuanxin Department of Clinical Laboratory, The Second Hospital Shandong University, Jinan, 250033, Province, China Humanistic Medicine Research Center, 250012, Qilu Hospital, These authors contributed equally to this work Correspondence to: Wang, email: cxwang@sdu.edu.cn Keywords: microRNA, lymph node metastasis, gastric cancer, prediction, prognosis...

Abstract Background Local relapses and metastases are primary causes of death in lung cancer patients. In the present study, we aimed to develop a prognostic signature based on metastasis‐associated lncRNAs patients with adenocarcinoma (LUAD). Methods Firstly, potential were identified by analyzing high‐throughput data from The Cancer Genome Atlas (TCGA), which, an lncRNA was constructed for prediction relapse LUAD using Cox proportional hazards regression analysis. Moreover, performance...

Abstract A group of unnatural 1-(2-deoxy-β-D-ribofuranosyl)-2,4-difluorobenzenes having a variety C-5 substituents (H, Me, F, Cl, Br, I, CF3, CN, NO2, NH2), designed as thymidine mimics, were synthesized for evaluation anticancer and antiviral agents. The coupling reaction 3,5-bis-O-(p-chlorobenzoyl)-2-deoxy-α-D-ribofuranosyl chloride with an organocadmium reagent [(2,4-difluorophenyl)2Cd] afforded mixture the α- β-anomeric products (α:β = 3:1 to 10:1 ratio). Treatment α-anomer BF3·Et2O in...

Deregulations of long non-coding RNAs (lncRNAs) have been implicated in the progression breast cancer (BC). However, prognostic values those lncRNAs BC remain elusive. This study aimed at constructing a lncRNA-based model to improve clinical management BC. Systematic investigation lncRNA expression profiles and data from The Cancer Genome Atlas (TCGA) database were utilized establish 10-lncRNA signature. signature efficiently discriminated patients with significantly different prognosis...

A group of unnatural 1-(2-deoxy-β-D-ribofuranosyl)-2,4-difluoro-benzenes having a variety C-5 two-carbon substituents [−C≡C−X, X = I, Br; −C≡CH;(E)−CH=CH−X, −CH=CH2; −CH2CH3; −CH(N3) CH2Br], designed as nucleoside mimics, were synthesized for evaluation anticancer and antiviral agents. The 5-substituted (E)−CH=CH−I −CH2CH3 compounds exhibited negligible cytotoxicity in MTT assay (CC50 10−3 to 10−4 M range), relative thymidine 10−5 against cancer cell lines. In contrast, the substituted...

Abstract A group of 1-[(2-hydroxyethoxy)methyl]- (12) and 1-[(1,3-dihydroxy-2-propoxy)methyl]- (13) derivatives 2,4-difluorobenzene possessing a variety C-5 substituents (R = Me, H, I, NO2) were designed with the expectation that they may serve as acyclic 5-substituted-2′-deoxyuridine (thymidine) mimics. Compounds 12 13 I) inactive anticancer agents (C50 10−3 to 10−4 M range), whereas 5-nitro compounds (12d, 13d) exhibited weak-to-moderate cytotoxicity (CC50 10−5 10−6 range) against cancer...

Objective To investigate the effect of perioperative nursing intervention on 30 cases premature with esophageal atresia.Methods 60 children congenital atresia treated in our hospital neonatal unit (NICU) from June 2008 to 2010, were randomly divided into group and control each group.The received routine postoperative care, this basis, given intervention.Results Complication rate was statistically significant difference (23.3% vs 60.0%, x2 =8.30,P ＜0.01); Hospital zation difference[(37.0 ±...