A5046784330- Corneal Surgery and Treatments
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Corneal surgery and disorders
- Bacteriophages and microbial interactions
- Advanced Glycation End Products research
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Cardiac Valve Diseases and Treatments
- Bacterial Genetics and Biotechnology
- Retinal Diseases and Treatments
- Ocular Surface and Contact Lens
- Cardiac Arrhythmias and Treatments
- Air Quality and Health Impacts
- Herpesvirus Infections and Treatments
- Genomics and Phylogenetic Studies
- Helicobacter pylori-related gastroenterology studies
- Connexins and lens biology
- NF-κB Signaling Pathways
- Protist diversity and phylogeny
- Antioxidant Activity and Oxidative Stress
- Proteoglycans and glycosaminoglycans research
- Ferroptosis and cancer prognosis
- Genetic Associations and Epidemiology
Jinan University
2015-2025
First Affiliated Hospital of Jinan University
2012-2025
North China University of Water Resources and Electric Power
2023-2025
Qilu Hospital of Shandong University
2014-2024
Hunan Provincial People's Hospital
2018-2022
Hunan Normal University
2022
Nanjing University of Aeronautics and Astronautics
2022
Guangzhou Medical University
2018
Shandong University
2009-2018
Zhejiang Taizhou Hospital
2018
Recent advantages of serum microRNAs (miRNAs) open a new realm possibilities for noninvasive diagnosis and prognosis bladder cancer (BC). The aim our study was to identify miRNA expression signatures in patients with BC establish models the recurrence prediction. We performed genome-wide analysis by Miseq sequencing followed evaluations training validation sets reverse transcription quantitative real-time PCR assays from samples 250 240 controls. A six-miRNA panel (miR-152, miR-148b-3p,...
// Weili Duan 1, * , Lutao Du Xiumei Jiang 1 Rui Wang Suzhen Yan Yujiao Xie Keqiang 2 Qingliang 3 Lili Xin Zhang Hongwei Pan Yongmei Yang Chuanxin Department of Clinical Laboratory, Qilu Hospital Shandong University, Jinan, 250012, Province, China Urology, Medical Affairs Management, These authors contributed equally to this work Correspondence to: Wang, email: cxwang@sdu.edu.cn Keywords: lncRNA, bladder cancer, diagnosis, recurrence, serum Received: July 25,...
Diabetic retinopathy (DR) has become a leading cause of global blindness as microvascular complication diabetes. Regular screening diabetic is strongly recommended for people with diabetes so that timely treatment can be provided to reduce the incidence visual impairment. However, DR not well carried out due lack eye care facilities, especially in rural areas China. Artificial intelligence (AI) based emerged novel strategy and show promising diagnostic performance sensitivity specificity,...
Macrophages are distributed throughout the body and crucial for restoration of damaged tissues. However, their characteristics in cornea roles repair corneal injures unclear. Here we show that macrophages can be classified as CCR2- macrophages, which already exist at embryonic day 12.5 (E12.5) similar to yolk sac-derived microglia, phenotype gene expression, CCR2+ do not appear until E17.5. At a steady state, have local proliferation capacity rarely affected by monocytes; however, following...
To assess the genotoxic effect of airborne particulate matter on corneal epithelial cells and investigate role reactive oxygen species (ROS) formation in this process.Immortalized human (HCECs) primary bovine were exposed to collected from Guangzhou for 24 hours. The cell viability toxicity measured by CCK-8 test lactate dehydrogenase (LDH) release, respectively. DNA breaks repair examined alkaline comet assay immunofluorescence staining phosphorylated histone variant H2AX (γH2AX), Reactive...
// Lutao Du 1 , Xiumei Jiang Weili Duan Rui Wang lishui Guixi Zheng Keqiang Yan 2 Lili Juan Li Xin Zhang Hongwei Pan Yongmei Yang and Chuanxin 3 Department of Clinical Laboratory, Qilu Hospital Shandong University, Jinan, 250012, Province, China Urology, The Second Jinan 250033, Shandong, Correspondence to: Wang, email: cxwang@sdu.edu.cn Keywords: microRNA, bladder cancer, diagnosis, recurrence, urine Received: October 22, 2016     Accepted: March 14,...
Aberrance in the blood bacterial microbiome has been identified and validated several non-infectious diseases, including cancer. The occurrence progression of gastric cancer found to be associated with alterations composition. However, composition patients is not well characterized. To test this hypothesis, we conducted a case-control study investigate microbiota compositions serum was investigated cancer, atypical hyperplasia, chronic gastritis, healthy control using 16S rRNA gene...
Inflammation and reepithelialization after corneal abrasion are critical for the rapid restoration of vision prevention microbial infections. However, endogenous regulatory mechanisms not completely understood. Here we report that manipulation autonomic nervous system (ANS) regulates inflammation healing processes. The activation sympathetic nerves inhibited but increased influx neutrophils release inflammatory cytokines. Conversely, parasympathetic promoted Furthermore, observed CD64+CCR2+...
Background: Cornea is the outmost structure of eye and exposed directly to air pollution. However, little known about effect PM2.5 on corneal epithelium, which critical for maintenance cornea homeostasis visual function.Objective: We investigated influence exposure epithelial migration possible mechanisms involved in process.Methods: observed wound healing mouse model abrasion, evaluated mobility cultured cells with scratch assay Transwell assay, detected phosphorylation interaction...
In mammalian corneal epithelium, mitosis shows a distinct circadian pattern. However, how this pattern is maintained, and it or its disruption influence renewal regeneration remain unclear.C57BL/6 mice were maintained under 12-hour light/12-hour dark (LD), light (LL), dark/12-hour (DD), reversed LD (DL, light; jet-lag defined as shift of 12 hours) conditions. Mitotic cells in epithelium enumerated analyzed via immunofluorescence at different zeitgeber times (ZTs). Expression core clock genes...
ABSTRACT The abandonment of paddy fields has become a significant concern in Asia, particularly, China's traditional rice‐growing regions. Although it affects the transformation soil carbon (C), nitrogen (N), and phosphorus (P), its long‐term effects remain unclear. A time‐series comparative analysis was conducted to examine subtropical on organic (SOC), total (TN), (TP), dissolved (DOC), (DON), available P (Olsen‐P). field experiment (1991–2022) region China (Taoyuan, Hunan Province)....
Serum microRNAs (miRNAs) have been proposed as novel non-invasive biomarkers for the early detection of cancer. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is most commonly used method investigating miRNA expression levels, however, interpretation RT-qPCR results depends largely on normalization to an appropriate endogenous control. The present study involved 129 patients with non-muscle-invasive bladder cancer (NMIBC), 121 muscle-invasive (MIBC) and 158 healthy...
Abstract The mechanisms of corneal epithelial lesions and delayed wound repair, as well their association with diabetes mellitus, are critical issues for clinical ophthalmologists. To test whether the diabetic condition alters circadian rhythm in a mouse cornea insulin can synchronise clock, we studied effects streptozotocin-induced on mitosis cells, recruitment leukocytes to cornea, expression main core clock genes ( Clock , Bmal1 Per2 Cry1 Rev-erbα ) epithelium. We also assessed possible...
Purpose: To identify the differently expressed micro (mi) RNAs in pterygium compared with normal conjunctiva and investigate potential role of miRNAs pathogenesis pterygium. Methods: With microRNA microarray quantitative RT-PCR, we identified that microRNA-122 (miR-122) was significantly decreased tissue. We detected expression Bcl-w, a predicted target miR-122, both conjunctiva, as well its correlation miR-122. Pterygium epithelial cells were isolated cultured, transfected miR-122 mimic or...