Serum microRNAs (miRNAs) have become a highlighted research hotspot, especially for their great potential as novel promising non-invasive biomarker in cancer diagnosis. The most frequently used approach serum miRNAs detection is quantitative real time polymerase chain reaction (qPCR). In order to obtain reliable qPCR data of expression, the use reference genes endogenous control undoubtly necessary. However, no systematic evaluation and validation normalizing analysis has been reported...

MicroRNA-210 (miR-210), the master hypoxamir, plays pleiotropic roles in certain cancers; however, its role development of human colorectal cancer remains unclear. Herein, we report that miR-210 is frequently up-regulated tissues, with high expression significantly correlating large tumor size, lymph node metastasis, advanced clinical stage and poor prognosis. Functionally, overexpression promotes migration invasion cells. Furthermore, can be induced by hypoxia mediates hypoxia-induced...

Abstract We previously identified five miRNAs (miR‐1, miR‐20a, miR‐27a, miR‐34a, and miR‐423‐5p) that are up‐regulated in gastric cancer. The goal of this study was to investigate the value these as potential biomarkers for predicting chemosensitivity prognosis metastatic or recurrent cancer patients who received first‐line chemotherapy. A total 82 with GC receiving chemotherapy were included our study. expression levels evaluated using hydrolysis probe‐based stem‐loop quantitative reverse...

Chemoresistance hinders the curative cancer chemotherapy. MicroRNAs (miRNAs) are key players in diverse biological processes including chemoresistance of cancers.A RNA-seq-based miR-omic analysis osteosarcoma (OS) cells was performed to detect levels miR-34a-5p. Bioinformatics revealed that AGTR1 is one target genes The mRNA and protein were detected both miR-34a-5p-mimic transfected G-292 miR-34a-5p-antagomiR SJSA-1 cells. involvement with OS validated by experiments siRNA-mediated...

Myocardial apoptosis plays an important role in doxorubicin (Dox) cardiotoxicity. MicroRNA-29 (miR-29) is suggested to function as anti-fibrotic factor with potential therapeutic effects on cardiac fibrosis. However, it has not been shown whether there association between miR-29b and myocardial apoptosis.Male Wistar rats were transfected agomir by local delivery the myocardium prior Dox treatment. Rat cardiomyocytes pretreated mimics or inhibitor followed incubation vitro. Cardiac underlying...

Abstract MiR-34a-5p has been implicated in the tumorigenesis and progression of several types cancer. However, role miR-34a-5p osteosarcoma (OS) remains largely unknown. This study was performed two multi-chemosensitive (G-292 MG63.2) resistant (SJSA-1 MNNG/HOS) OS cell lines. promotes multi-chemoresistance via its repression Delta-like ligand 1 (DLL1) gene, Notch pathway, thus negatively correlates with chemoresistance. The siRNA-mediated DLL1 gene suppressed apoptosis de-sensitized G-292...

Chemoresistance hinders curative cancer chemotherapy in osteosarcoma (OS), resulting only an approximately 20 % survival rate patients with metastatic disease at diagnosis. Identifying the mechanisms responsible for regulating resistance is crucial improving OS treatment.This study was performed two human cell lines (the multi-chemosensitive line G-292 and multi-chemoresistant SJSA-1). The levels of miR-20a-5p KIF26B mRNA expression were determined by quantitative real-time PCR. protein...

An association has been reported between miR-34a-5p and several types of cancer. Specifically, in this study, using systematic observations multi-drug sensitive (G-292 MG63.2) resistant (SJSA-1 MNNG/HOS) osteosarcoma (OS) cell lines, we showed that promotes the resistance OS through receptor tyrosine kinase CD117, a direct target miR-34a-5p. Consistently, siRNA-mediated repression CD117 G-292 MG63.2 cells led to similar phenotype exhibited all mimic-triggered changes. In addition, activity...

Background: Thyroid cancer (TC) is considered as the fastest growing malignancy in human endocrine system, particularly papillary thyroid (PTC). MicroRNAs (miRs) serve a role promoting or suppressing tumors various types of malignant tumor including PTC. This study aims to explore whether microRNA-26a-5p (miR-26a-5p) could affect proliferation, invasion and metastasis ability PTC cells by regulating Wnt5a. Materials methods: The expression miR-26a-5p was examined qRT-PCR tissue samples (58...

Abstract Purpose Cisplatin is a widely used and effective chemotherapeutic agent for most solid malignant tumors. However, cisplatin-induced ototoxicity common adverse effect that limits the therapeutic efficacy of tumors in clinic. To date, specific mechanism has not been fully elucidated, management also an urgent challenge. Recently, some authors believed miR34a mitophagy played role age-related drug-induced hearing loss. Our study aimed to explore involvement miR-34a/DRP-1-mediated...

<i>Background:</i> Vascular endothelial growth factor (VEGF) is upregulated by hypoxia and a major stimulatory for choroidal neovascularization. The upregulation of VEGF expression in response to occurs through hypoxia-inducible 1 (HIF-1), which transcription that regulates genes involved the hypoxia. HIF-1α inducible subunit HIF-1. <i>Aims:</i> To further define HIF-1 function angiogenesis explore novel approaches modulate neovascularization age-related macular...

Serum microRNAs (miRNAs) have been proposed as novel non-invasive biomarkers for the early detection of cancer. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is most commonly used method investigating miRNA expression levels, however, interpretation RT-qPCR results depends largely on normalization to an appropriate endogenous control. The present study involved 129 patients with non-muscle-invasive bladder cancer (NMIBC), 121 muscle-invasive (MIBC) and 158 healthy...

As one of the hallmarks cancer, chemoresistance hinders curative cancer chemotherapy in osteosarcoma (OS). MicroRNAs (miRNAs) act as key regulators gene expression diverse biological processes including multi-chemoresistance cancers.Based on CCK8 experiments, we performed an RNA-seq-based miR-omic analysis (OS) cells (a multi-chemosensitive OS cell line G-292 and a multi-chemoresistant SJSA-1) to detect levels miR-20a-5p. We predicted Homo sapiens syndecan 2 (SDC2) target genes miR-20a-5p...

Myocardial blood flow (MBF) provides important diagnostic information for myocardial ischemia. However, dynamic computed tomography perfusion (CTP) needed MBF involves multiple exposures, leading to high radiation doses. This study investigated synthesizing from simulated static CTP explore dose reduction potential, bypassing the traditional input function. The included 253 subjects with intermediate-to-high pretest probabilities of obstructive coronary artery disease (CAD). was...

Abstract Background and objectives Micro RNA ‐9 is frequently dysregulated in many human carcinoma types, including gastric cancer (GC). Previous studies demonstrated that the expression of TNFAIP 8 GC correlated with tumour occurrence, development, invasion, metastasis prognosis. However, till now, relationship between has not been reported. Materials methods Levels miR‐9 tissues cell lines were studied using qualitative real‐time PCR ( qRT ‐ ) Western blotting. Cell viability was detected...

The aim of the present study was to examine effect fraxetin on proliferation and apoptosis in MCF-7 breast cancer cell line. Cell measused using an MTT assay 4',6-diamidino-2-phenylindole (DAPI) staining used determine shrinkage condensation. RT-PCR expression factor‑associated suicide (Fas) Fas ligand (FasL) mRNA, western blot analysis Bax Bcl-2 protein. showed that cells significantly inhibited by a dose-dependent manner. Fraxetin also induced significant morphological changes cells,...

Osteosarcoma (OS) is the most prevalent primary malignant bone tumor in children and young adults, its complex etiology involving a combination of environmental genetic factors. MicroRNA (miRNA) short, non‑coding regulatory RNA molecule that represses gene expression by imperfectly base‑pairing to 3' untranslated region target mRNAs. Evidence has shown alterations miRNA are involved initiation, progression, metastasis human cancers. It believed miRNAs function both as suppressors oncogenes...

Recently, microRNA-96-5p (miR-96-5p) has been reported to function as both a tumor suppressor and oncogene in several cancer types, including gastric cancer, hepatocellular lung cancer. However, the biological of miR-96-5p its precise mechanisms oral squamous cell carcinoma (OSCC) have not well clarified. The aim this study was roles miR-96-5p/FOXF2 axis OSCC. In study, level dramatically enhanced OSCC tissues lines, FOXF2 expression significantly reduced. addition, negatively related...

Identification of hub genes and key pathways gastric cancer was recognized to be essential elucidate the tumorigenesis GC.This study aimed identify differentially expressed (DEGs) in GC via bioinformatics methods their related involved pathological process GC.Gene expression profile datasets acquired by microarray chips or RNA-seq were downloaded from GEO dataset TCGA, 298 identified.The Gene Ontology (GO) Kyoto Genomic Encyclopedia (KEGG) DEGs then analyzed DAVID database potential...

Chemoresistance prevents curative potential of chemotherapy in most cases. MicroRNAs (miRNAs) are key players regulating chemoresistance osteosarcoma, which is the common primary bone cancer. Bisulfite sequencing and quantitative real time PCR analyses showed that miR-193a expression downregulated by DNA hypermethylation at its promoter region a chemoresistant cell line, SJSA-1, compared to chemosensitive line G-292. Introduction mimic SJSA-1 cells or an antagomir into G-292 confirmed role...

MicroRNAs (miRNAs) modulate complex physiological and pathological processes, including the regulation of angiogenesis. Our previous study reported that bone marrow-derived mesenchymal stem cells (MSCs) are recruited into choroidal neovascularization lesions. miRNA-195 is highly expressed in MSCs, but its function remains unknown. In present study, miR-195a-3p abundance was significantly decreased hypoxia-treated murine MSCs; on other hand, overexpression reduced MSC proliferation migration...

Objectives This study aims to compare the microbiota of gingival crevicular fluid (GCF) before and after mechanical debridement (MD) with antimicrobial photodynamic therapy (aPDT) determine core efficient in peri-implantitis treatment. Methods We recruited 9 patients (14 implants) treated MD+aPDT for at our center from February 1, 2018, 2019. GCF was collected using filter paper strip The bacterial 16S rRNA amplified sequenced an Illumina MiSeq platform characterize GCF. Bioinformatics...

Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence 4‑5 cases/100,000,000. The 5-year survival rate patients osteosarcoma has significantly improved over time; however, there remains a significant proportion that respond poorly to chemotherapy. An understanding pathology required provide more effective treatment strategies, identify biomarkers develop novel...